Comparative transcriptome analysis of resistant, moderately resistant, and susceptible wheat-near-isogenic lines in response to Puccinia striiformis tritici
Stripe/yellow rust is caused by a biotrophic fungal pathogen known as Puccinia striiformis f. sp.tritici (Pst). RNA seq technique was used to understand the wheat and stripe rust interaction in three different reaction types. Leave samples of three near-isogenic lines (NILs) of wheat showing Resista...
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Plant Interactions |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17429145.2025.2481853 |
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| author | Zainy Zainy Muhammad Zeeshan Hyder Uzma Uzma Muhammad Fayyaz Umer Zeeshan Ijaz Sumaira Farrakh |
| author_facet | Zainy Zainy Muhammad Zeeshan Hyder Uzma Uzma Muhammad Fayyaz Umer Zeeshan Ijaz Sumaira Farrakh |
| author_sort | Zainy Zainy |
| collection | DOAJ |
| description | Stripe/yellow rust is caused by a biotrophic fungal pathogen known as Puccinia striiformis f. sp.tritici (Pst). RNA seq technique was used to understand the wheat and stripe rust interaction in three different reaction types. Leave samples of three near-isogenic lines (NILs) of wheat showing Resistant/immune (R), Moderately Resistant (MR), and Susceptible (S) (inoculated with Puccinia striiformis race 574232) responses were collected 48 and 72 h after inoculation (hai). Overall, 8,595 DEGs were upregulated and 8,741 were downregulated with high numbers (4,357) of DEGs identified S-R in comparison at 72hai. Subsequent Gene Ontology (GO) enrichment analysis suggests protein phosphorylation, biological process, and cellular process of internal components of membrane enriched in all three comparisons at both time points. DEGs identified as serine/threonine receptor-like kinases, peroxidases, Mitogen-phosphate Kinases (MAPK), Phenylalanine ammonia-lyase (PAL), and Pathogenesis-related protein (PR) were high in resistant NIL in all comparisons at both time points. The results show that although the responses of the near iso-genic lines are similar, it is the magnitude of the response that plays a role in the differences in their resistance to Puccinia striiformis. |
| format | Article |
| id | doaj-art-59d7cb3b409f481b9241e239ef00c180 |
| institution | OA Journals |
| issn | 1742-9145 1742-9153 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
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| series | Journal of Plant Interactions |
| spelling | doaj-art-59d7cb3b409f481b9241e239ef00c1802025-08-20T01:50:15ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532025-12-0120110.1080/17429145.2025.2481853Comparative transcriptome analysis of resistant, moderately resistant, and susceptible wheat-near-isogenic lines in response to Puccinia striiformis triticiZainy Zainy0Muhammad Zeeshan Hyder1Uzma Uzma2Muhammad Fayyaz3Umer Zeeshan Ijaz4Sumaira Farrakh5Department of Biosciences, COMSATS University Islamabad, Islamabad, PakistanDepartment of Biosciences, COMSATS University Islamabad, Islamabad, PakistanWater & Environment Research Group, Mazumdar-Shaw Advanced Research Centre, University of Glasgow, Glasgow, UKNational Agriculture Research Center, Islamabad, PakistanWater & Environment Research Group, Mazumdar-Shaw Advanced Research Centre, University of Glasgow, Glasgow, UKDepartment of Biosciences, COMSATS University Islamabad, Islamabad, PakistanStripe/yellow rust is caused by a biotrophic fungal pathogen known as Puccinia striiformis f. sp.tritici (Pst). RNA seq technique was used to understand the wheat and stripe rust interaction in three different reaction types. Leave samples of three near-isogenic lines (NILs) of wheat showing Resistant/immune (R), Moderately Resistant (MR), and Susceptible (S) (inoculated with Puccinia striiformis race 574232) responses were collected 48 and 72 h after inoculation (hai). Overall, 8,595 DEGs were upregulated and 8,741 were downregulated with high numbers (4,357) of DEGs identified S-R in comparison at 72hai. Subsequent Gene Ontology (GO) enrichment analysis suggests protein phosphorylation, biological process, and cellular process of internal components of membrane enriched in all three comparisons at both time points. DEGs identified as serine/threonine receptor-like kinases, peroxidases, Mitogen-phosphate Kinases (MAPK), Phenylalanine ammonia-lyase (PAL), and Pathogenesis-related protein (PR) were high in resistant NIL in all comparisons at both time points. The results show that although the responses of the near iso-genic lines are similar, it is the magnitude of the response that plays a role in the differences in their resistance to Puccinia striiformis.https://www.tandfonline.com/doi/10.1080/17429145.2025.2481853Wheat isogenic linesresistantmoderately resistantsusceptiblePuccinia striiformisRNA-seq analysis |
| spellingShingle | Zainy Zainy Muhammad Zeeshan Hyder Uzma Uzma Muhammad Fayyaz Umer Zeeshan Ijaz Sumaira Farrakh Comparative transcriptome analysis of resistant, moderately resistant, and susceptible wheat-near-isogenic lines in response to Puccinia striiformis tritici Journal of Plant Interactions Wheat isogenic lines resistant moderately resistant susceptible Puccinia striiformis RNA-seq analysis |
| title | Comparative transcriptome analysis of resistant, moderately resistant, and susceptible wheat-near-isogenic lines in response to Puccinia striiformis tritici |
| title_full | Comparative transcriptome analysis of resistant, moderately resistant, and susceptible wheat-near-isogenic lines in response to Puccinia striiformis tritici |
| title_fullStr | Comparative transcriptome analysis of resistant, moderately resistant, and susceptible wheat-near-isogenic lines in response to Puccinia striiformis tritici |
| title_full_unstemmed | Comparative transcriptome analysis of resistant, moderately resistant, and susceptible wheat-near-isogenic lines in response to Puccinia striiformis tritici |
| title_short | Comparative transcriptome analysis of resistant, moderately resistant, and susceptible wheat-near-isogenic lines in response to Puccinia striiformis tritici |
| title_sort | comparative transcriptome analysis of resistant moderately resistant and susceptible wheat near isogenic lines in response to puccinia striiformis tritici |
| topic | Wheat isogenic lines resistant moderately resistant susceptible Puccinia striiformis RNA-seq analysis |
| url | https://www.tandfonline.com/doi/10.1080/17429145.2025.2481853 |
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