Gene Silencing of Sarco/Endoplasmic Reticulum Ca<sup>2+</sup>-ATPase and NADPH–Cytochrome P450 Reductase as a Novel Approach to <i>Leptinotarsa decemlineata</i> Management
The Colorado Potato Beetle (CPB, <i>Leptinotarsa decemlineata</i> Say, Coleoptera: Chrysomelidae) remains a destructive agricultural pest worldwide that continually overcomes conventional control methods. In recent years, RNA interference (RNAi) has emerged as an alternative for its mana...
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2025-05-01
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| author | Maribel Mendoza-Alatorre Tania Samanta Siqueiros-Cendón Jorge Ariel Torres-Castillo Sugey Ramona Sinagawa-García Blanca Flor Iglesias-Figueroa María Jazmín Abraham-Juárez Carmen Daniela González-Barriga Quintín Rascón-Cruz Luis Ignacio Siañez-Estrada Edward Alexander Espinoza-Sánchez |
| author_facet | Maribel Mendoza-Alatorre Tania Samanta Siqueiros-Cendón Jorge Ariel Torres-Castillo Sugey Ramona Sinagawa-García Blanca Flor Iglesias-Figueroa María Jazmín Abraham-Juárez Carmen Daniela González-Barriga Quintín Rascón-Cruz Luis Ignacio Siañez-Estrada Edward Alexander Espinoza-Sánchez |
| author_sort | Maribel Mendoza-Alatorre |
| collection | DOAJ |
| description | The Colorado Potato Beetle (CPB, <i>Leptinotarsa decemlineata</i> Say, Coleoptera: Chrysomelidae) remains a destructive agricultural pest worldwide that continually overcomes conventional control methods. In recent years, RNA interference (RNAi) has emerged as an alternative for its management; however, although promising results have been reported, its effectiveness has been influenced by several factors, including the length of double-stranded RNA (dsRNA), the delivery method, stability, and especially the selection of the target gene. In this study, we designed and synthesized 290 bp dsRNAs targeting the <i>SERCA</i> and <i>CPR</i> genes from <i>L. decemlineata</i>, which encode the Sarco/Endoplasmic Reticulum Ca<sup>2</sup>⁺-ATPase and NADPH–Cytochrome P450 Reductase, respectively. Both dsRNAs successfully reduced transcript levels in larvae, with ds<i>SERCA</i> achieving ~60% knockdown by day 3 and ds<i>CPR</i> achieving ~50% knockdown by day 7. Furthermore, both treatments affected the larval growth and survival rate. However, while the ds<i>CPR</i>-treated larvae showed a 59% reduction in weight gain, the administration of ds<i>SERCA</i> had a strong phenotypic effect on the larvae, leading to decreased feeding, a 50.4% reduction in weight gain, and ultimately, 100% mortality. These results suggest that the <i>SERCA</i> and <i>CPR</i> genes could be promising targets for <i>L. decemlineata</i> control and emphasize the importance of appropriate target gene selection for RNAi silencing, as well as the need to explore and validate new genes for RNAi-mediated pest management. |
| format | Article |
| id | doaj-art-59d2c01be4514ee8bdfc12a4b768ef80 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-59d2c01be4514ee8bdfc12a4b768ef802025-08-20T03:47:48ZengMDPI AGAgronomy2073-43952025-05-01155115110.3390/agronomy15051151Gene Silencing of Sarco/Endoplasmic Reticulum Ca<sup>2+</sup>-ATPase and NADPH–Cytochrome P450 Reductase as a Novel Approach to <i>Leptinotarsa decemlineata</i> ManagementMaribel Mendoza-Alatorre0Tania Samanta Siqueiros-Cendón1Jorge Ariel Torres-Castillo2Sugey Ramona Sinagawa-García3Blanca Flor Iglesias-Figueroa4María Jazmín Abraham-Juárez5Carmen Daniela González-Barriga6Quintín Rascón-Cruz7Luis Ignacio Siañez-Estrada8Edward Alexander Espinoza-Sánchez9Laboratorio de Biotecnología I, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario S/N Nuevo Campus Universitario, Chihuahua C.P. 31125, CH, MexicoLaboratorio de Biotecnología I, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario S/N Nuevo Campus Universitario, Chihuahua C.P. 31125, CH, MexicoInstituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, Ave. División del Golfo 356, Col. Libertad, Ciudad Victoria C.P. 87019, TA, MexicoLaboratorio de Biotecnología, Facultad de Agronomía, Universidad Autónoma de Nuevo León, Francisco Villa S/N Col. Ex Hacienda El Canadá, General Escobedo C.P. 66050, NL, MexicoLaboratorio de Biotecnología I, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario S/N Nuevo Campus Universitario, Chihuahua C.P. 31125, CH, MexicoLaboratorio de Biología Molecular del Desarrollo, Centro de Investigación y de Estudios Avanzados del IPN, Libramiento Norte León Km 9.6, Irapuato C.P. 36821, GT, MexicoLaboratorio de Cultivo de Tejidos, División de Ingeniería y Ciencias, Tecnológico de Monterrey, Av. Heroico Colegio Militar 4700, Nombre de Dios, Chihuahua C.P. 31100, CH, MexicoLaboratorio de Biotecnología I, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario S/N Nuevo Campus Universitario, Chihuahua C.P. 31125, CH, MexicoLaboratorio de Biotecnología I, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario S/N Nuevo Campus Universitario, Chihuahua C.P. 31125, CH, MexicoLaboratorio de Biotecnología I, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario S/N Nuevo Campus Universitario, Chihuahua C.P. 31125, CH, MexicoThe Colorado Potato Beetle (CPB, <i>Leptinotarsa decemlineata</i> Say, Coleoptera: Chrysomelidae) remains a destructive agricultural pest worldwide that continually overcomes conventional control methods. In recent years, RNA interference (RNAi) has emerged as an alternative for its management; however, although promising results have been reported, its effectiveness has been influenced by several factors, including the length of double-stranded RNA (dsRNA), the delivery method, stability, and especially the selection of the target gene. In this study, we designed and synthesized 290 bp dsRNAs targeting the <i>SERCA</i> and <i>CPR</i> genes from <i>L. decemlineata</i>, which encode the Sarco/Endoplasmic Reticulum Ca<sup>2</sup>⁺-ATPase and NADPH–Cytochrome P450 Reductase, respectively. Both dsRNAs successfully reduced transcript levels in larvae, with ds<i>SERCA</i> achieving ~60% knockdown by day 3 and ds<i>CPR</i> achieving ~50% knockdown by day 7. Furthermore, both treatments affected the larval growth and survival rate. However, while the ds<i>CPR</i>-treated larvae showed a 59% reduction in weight gain, the administration of ds<i>SERCA</i> had a strong phenotypic effect on the larvae, leading to decreased feeding, a 50.4% reduction in weight gain, and ultimately, 100% mortality. These results suggest that the <i>SERCA</i> and <i>CPR</i> genes could be promising targets for <i>L. decemlineata</i> control and emphasize the importance of appropriate target gene selection for RNAi silencing, as well as the need to explore and validate new genes for RNAi-mediated pest management.https://www.mdpi.com/2073-4395/15/5/1151<i>Leptinotarsa decemlineata</i><i>SERCA</i> gene<i>CPR</i> geneRNA interferencetranscript levelsmortality |
| spellingShingle | Maribel Mendoza-Alatorre Tania Samanta Siqueiros-Cendón Jorge Ariel Torres-Castillo Sugey Ramona Sinagawa-García Blanca Flor Iglesias-Figueroa María Jazmín Abraham-Juárez Carmen Daniela González-Barriga Quintín Rascón-Cruz Luis Ignacio Siañez-Estrada Edward Alexander Espinoza-Sánchez Gene Silencing of Sarco/Endoplasmic Reticulum Ca<sup>2+</sup>-ATPase and NADPH–Cytochrome P450 Reductase as a Novel Approach to <i>Leptinotarsa decemlineata</i> Management Agronomy <i>Leptinotarsa decemlineata</i> <i>SERCA</i> gene <i>CPR</i> gene RNA interference transcript levels mortality |
| title | Gene Silencing of Sarco/Endoplasmic Reticulum Ca<sup>2+</sup>-ATPase and NADPH–Cytochrome P450 Reductase as a Novel Approach to <i>Leptinotarsa decemlineata</i> Management |
| title_full | Gene Silencing of Sarco/Endoplasmic Reticulum Ca<sup>2+</sup>-ATPase and NADPH–Cytochrome P450 Reductase as a Novel Approach to <i>Leptinotarsa decemlineata</i> Management |
| title_fullStr | Gene Silencing of Sarco/Endoplasmic Reticulum Ca<sup>2+</sup>-ATPase and NADPH–Cytochrome P450 Reductase as a Novel Approach to <i>Leptinotarsa decemlineata</i> Management |
| title_full_unstemmed | Gene Silencing of Sarco/Endoplasmic Reticulum Ca<sup>2+</sup>-ATPase and NADPH–Cytochrome P450 Reductase as a Novel Approach to <i>Leptinotarsa decemlineata</i> Management |
| title_short | Gene Silencing of Sarco/Endoplasmic Reticulum Ca<sup>2+</sup>-ATPase and NADPH–Cytochrome P450 Reductase as a Novel Approach to <i>Leptinotarsa decemlineata</i> Management |
| title_sort | gene silencing of sarco endoplasmic reticulum ca sup 2 sup atpase and nadph cytochrome p450 reductase as a novel approach to i leptinotarsa decemlineata i management |
| topic | <i>Leptinotarsa decemlineata</i> <i>SERCA</i> gene <i>CPR</i> gene RNA interference transcript levels mortality |
| url | https://www.mdpi.com/2073-4395/15/5/1151 |
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