Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue
Macrophages are important components of adipose tissue inflammation, which results in metabolic diseases such as insulin resistance. Notably, obesity induces a proinflammatory phenotypic switch in adipose tissue macrophages, and oxidative stress facilitates this switch. Thus, we examined the role of...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/8675905 |
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| author | Ye Seul Park Md Jamal Uddin Lingjuan Piao Inah Hwang Jung Hwa Lee Hunjoo Ha |
| author_facet | Ye Seul Park Md Jamal Uddin Lingjuan Piao Inah Hwang Jung Hwa Lee Hunjoo Ha |
| author_sort | Ye Seul Park |
| collection | DOAJ |
| description | Macrophages are important components of adipose tissue inflammation, which results in metabolic diseases such as insulin resistance. Notably, obesity induces a proinflammatory phenotypic switch in adipose tissue macrophages, and oxidative stress facilitates this switch. Thus, we examined the role of endogenous catalase, a key regulator of oxidative stress, in the activity of adipose tissue macrophages in obese mice. Catalase knockout (CKO) exacerbated insulin resistance, amplified oxidative stress, and accelerated macrophage infiltration into epididymal white adipose tissue in mice on normal or high-fat diet. Interestingly, catalase deficiency also enhanced classical macrophage activation (M1) and inflammation but suppressed alternative activation (M2) regardless of diet. Similarly, pharmacological inhibition of catalase activity using 3-aminotriazole induced the same phenotypic switch and inflammatory response in RAW264.7 macrophages. Finally, the same phenotypic switch and inflammatory responses were observed in primary bone marrow-derived macrophages from CKO mice. Taken together, the data indicate that endogenous catalase regulates the polarization of adipose tissue macrophages and thereby inhibits inflammation and insulin resistance. |
| format | Article |
| id | doaj-art-59c79c8776b240ebabe0720dfba21fe3 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-59c79c8776b240ebabe0720dfba21fe32025-02-03T01:09:37ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/86759058675905Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose TissueYe Seul Park0Md Jamal Uddin1Lingjuan Piao2Inah Hwang3Jung Hwa Lee4Hunjoo Ha5Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of KoreaGraduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of KoreaGraduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of KoreaGraduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of KoreaGraduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of KoreaGraduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of KoreaMacrophages are important components of adipose tissue inflammation, which results in metabolic diseases such as insulin resistance. Notably, obesity induces a proinflammatory phenotypic switch in adipose tissue macrophages, and oxidative stress facilitates this switch. Thus, we examined the role of endogenous catalase, a key regulator of oxidative stress, in the activity of adipose tissue macrophages in obese mice. Catalase knockout (CKO) exacerbated insulin resistance, amplified oxidative stress, and accelerated macrophage infiltration into epididymal white adipose tissue in mice on normal or high-fat diet. Interestingly, catalase deficiency also enhanced classical macrophage activation (M1) and inflammation but suppressed alternative activation (M2) regardless of diet. Similarly, pharmacological inhibition of catalase activity using 3-aminotriazole induced the same phenotypic switch and inflammatory response in RAW264.7 macrophages. Finally, the same phenotypic switch and inflammatory responses were observed in primary bone marrow-derived macrophages from CKO mice. Taken together, the data indicate that endogenous catalase regulates the polarization of adipose tissue macrophages and thereby inhibits inflammation and insulin resistance.http://dx.doi.org/10.1155/2016/8675905 |
| spellingShingle | Ye Seul Park Md Jamal Uddin Lingjuan Piao Inah Hwang Jung Hwa Lee Hunjoo Ha Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue Mediators of Inflammation |
| title | Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue |
| title_full | Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue |
| title_fullStr | Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue |
| title_full_unstemmed | Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue |
| title_short | Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue |
| title_sort | novel role of endogenous catalase in macrophage polarization in adipose tissue |
| url | http://dx.doi.org/10.1155/2016/8675905 |
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