The Role of Gut Microbiota in Male Erectile Dysfunction of Rats
Purpose: Erectile dysfunction (ED) is a common male sexual dysfunction. Gut microbiota plays an important role in various diseases. To investigate the effects and mechanisms of intestinal flora dysregulation induced by high-fat diet (HFD) on erectile function. Materials and Methods: Male Sprague–...
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| Format: | Article |
| Language: | English |
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Korean Society for Sexual Medicine and Andrology
2025-01-01
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| Series: | The World Journal of Men's Health |
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| author | Zhunan Xu Shangren Wang Chunxiang Liu Jiaqi Kang Yang Pan Zhexin Zhang Hang Zhou Mingming Xu Xia Li Haoyu Wang Shuai Niu Li Liu Daqing Sun Xiaoqiang Liu |
| author_facet | Zhunan Xu Shangren Wang Chunxiang Liu Jiaqi Kang Yang Pan Zhexin Zhang Hang Zhou Mingming Xu Xia Li Haoyu Wang Shuai Niu Li Liu Daqing Sun Xiaoqiang Liu |
| author_sort | Zhunan Xu |
| collection | DOAJ |
| description | Purpose: Erectile dysfunction (ED) is a common male sexual dysfunction. Gut microbiota plays an important role in various
diseases. To investigate the effects and mechanisms of intestinal flora dysregulation induced by high-fat diet (HFD) on erectile
function.
Materials and Methods: Male Sprague–Dawley rats aged 8 weeks were randomly divided into the normal diet (ND) and HFD
groups. After 24 weeks, a measurement of erectile function was performed. We performed 16S rRNA sequencing of stool
samples. Then, we established fecal microbiota transplantation (FMT) rat models by transplanting fecal microbiota from rats
of ND group and HFD group to two new groups of rats respectively. After 24 weeks, erectile function of the rats was evaluated
and 16S rRNA sequencing was performed, and serum samples were collected for the untargeted metabolomics detection.
Results: The erectile function of rats and the species diversity of intestinal microbiota in the HFD group was significantly lower,
and the characteristics of the intestinal microbiota community structure were also significantly different between the two
groups. The erectile function of rats in the HFD-FMT group was significantly lower than that of rats in the ND-FMT group.
The characteristics of the intestinal microbiota community structure were significantly different. In the HFD-FMT group, 27
metabolites were significantly different and they were mainly involved in the several inflammation-related pathways.
Conclusions: Intestinal microbiota disorders induced by HFD can damage the intestinal barrier of rats, change the serum
metabolic profile, induce low-grade inflammation and apoptosis in the corpus cavernosum of the penis, and lead to ED. |
| format | Article |
| id | doaj-art-59c41b9794b0422c960789457900427c |
| institution | Kabale University |
| issn | 2287-4208 2287-4690 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Korean Society for Sexual Medicine and Andrology |
| record_format | Article |
| series | The World Journal of Men's Health |
| spelling | doaj-art-59c41b9794b0422c960789457900427c2025-01-03T05:21:36ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902025-01-0143121322710.5534/wjmh.230337The Role of Gut Microbiota in Male Erectile Dysfunction of RatsZhunan Xu0https://orcid.org/0000-0002-6457-4762Shangren Wang1https://orcid.org/0000-0003-3412-7758Chunxiang Liu2https://orcid.org/0000-0001-7520-1702Jiaqi Kang3https://orcid.org/0000-0003-4191-854XYang Pan4https://orcid.org/0000-0003-3962-4278Zhexin Zhang5https://orcid.org/0000-0001-6553-7894Hang Zhou6https://orcid.org/0009-0008-2195-1549Mingming Xu7https://orcid.org/0009-0007-4167-3729Xia Li8https://orcid.org/0000-0002-1254-4318Haoyu Wang9https://orcid.org/0000-0002-7055-2033Shuai Niu10https://orcid.org/0000-0002-9358-4572Li Liu11https://orcid.org/0000-0002-4970-2834Daqing Sun12https://orcid.org/0000-0001-9578-8790Xiaoqiang Liu13https://orcid.org/0000-0003-3524-6783Department of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Pediatric Surgery, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Pediatric Surgery, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Urology, Tianjin Medical University General Hospital, Tianjin, ChinaPurpose: Erectile dysfunction (ED) is a common male sexual dysfunction. Gut microbiota plays an important role in various diseases. To investigate the effects and mechanisms of intestinal flora dysregulation induced by high-fat diet (HFD) on erectile function. Materials and Methods: Male Sprague–Dawley rats aged 8 weeks were randomly divided into the normal diet (ND) and HFD groups. After 24 weeks, a measurement of erectile function was performed. We performed 16S rRNA sequencing of stool samples. Then, we established fecal microbiota transplantation (FMT) rat models by transplanting fecal microbiota from rats of ND group and HFD group to two new groups of rats respectively. After 24 weeks, erectile function of the rats was evaluated and 16S rRNA sequencing was performed, and serum samples were collected for the untargeted metabolomics detection. Results: The erectile function of rats and the species diversity of intestinal microbiota in the HFD group was significantly lower, and the characteristics of the intestinal microbiota community structure were also significantly different between the two groups. The erectile function of rats in the HFD-FMT group was significantly lower than that of rats in the ND-FMT group. The characteristics of the intestinal microbiota community structure were significantly different. In the HFD-FMT group, 27 metabolites were significantly different and they were mainly involved in the several inflammation-related pathways. Conclusions: Intestinal microbiota disorders induced by HFD can damage the intestinal barrier of rats, change the serum metabolic profile, induce low-grade inflammation and apoptosis in the corpus cavernosum of the penis, and lead to ED.diet; high-faterectile dysfunctiongastrointestinal microbiomeinflammationmetabolomics |
| spellingShingle | Zhunan Xu Shangren Wang Chunxiang Liu Jiaqi Kang Yang Pan Zhexin Zhang Hang Zhou Mingming Xu Xia Li Haoyu Wang Shuai Niu Li Liu Daqing Sun Xiaoqiang Liu The Role of Gut Microbiota in Male Erectile Dysfunction of Rats The World Journal of Men's Health diet; high-fat erectile dysfunction gastrointestinal microbiome inflammation metabolomics |
| title | The Role of Gut Microbiota in Male Erectile Dysfunction of Rats |
| title_full | The Role of Gut Microbiota in Male Erectile Dysfunction of Rats |
| title_fullStr | The Role of Gut Microbiota in Male Erectile Dysfunction of Rats |
| title_full_unstemmed | The Role of Gut Microbiota in Male Erectile Dysfunction of Rats |
| title_short | The Role of Gut Microbiota in Male Erectile Dysfunction of Rats |
| title_sort | role of gut microbiota in male erectile dysfunction of rats |
| topic | diet; high-fat erectile dysfunction gastrointestinal microbiome inflammation metabolomics |
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