Molecular and serological survey of Trypanosoma vivax in Crioulo Lageano Cattle from southern Brazil

Abstract Bovine trypanosomiasis, caused by infection with the protozoan Trypanosoma vivax, is harmful to livestock worldwide. Knowing its epidemiology is relevant to evaluate the susceptibility, resistance, and tolerance of animals. The objective of this study was to determine the prevalence of T. v...

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Main Authors: Felipe Eduardo Fiorin, Mariana da Silva Casa, Leonardo Bergmann Griebeler, Mariana Fuchs Goedel, Gianlucca Simão Nadal Ribeiro, Luiz Flávio Nepomuceno do Nascimento, Gabriella Bassi das Neves, Graziela Vieira Fonteque, Luiz Cláudio Miletti, Mere Erika Saito, Joandes Henrique Fonteque
Format: Article
Language:English
Published: Colégio Brasileiro de Parasitologia Veterinaria 2025-04-01
Series:Revista Brasileira de Parasitologia Veterinária
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612025000100406&lng=en&tlng=en
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author Felipe Eduardo Fiorin
Mariana da Silva Casa
Leonardo Bergmann Griebeler
Mariana Fuchs Goedel
Gianlucca Simão Nadal Ribeiro
Luiz Flávio Nepomuceno do Nascimento
Gabriella Bassi das Neves
Graziela Vieira Fonteque
Luiz Cláudio Miletti
Mere Erika Saito
Joandes Henrique Fonteque
author_facet Felipe Eduardo Fiorin
Mariana da Silva Casa
Leonardo Bergmann Griebeler
Mariana Fuchs Goedel
Gianlucca Simão Nadal Ribeiro
Luiz Flávio Nepomuceno do Nascimento
Gabriella Bassi das Neves
Graziela Vieira Fonteque
Luiz Cláudio Miletti
Mere Erika Saito
Joandes Henrique Fonteque
author_sort Felipe Eduardo Fiorin
collection DOAJ
description Abstract Bovine trypanosomiasis, caused by infection with the protozoan Trypanosoma vivax, is harmful to livestock worldwide. Knowing its epidemiology is relevant to evaluate the susceptibility, resistance, and tolerance of animals. The objective of this study was to determine the prevalence of T. vivax in Crioula Lageana cattle and relate them to clinical, hematological, and biochemical findings to elucidate the breed’s health and disease tolerance characteristics. Venous blood samples from 310 bovines considered healthy during the clinical examination were used to perform polymerase chain reaction (PCR) analysis, Immunofluorescence Antibody Test (IFAT) assays, hemogram tests, and serum biochemistry. The collected data were subjected to statistical analyses to compare seropositive and negative groups. IFAT indicated that the seroprevalence for T. vivax was 8% (24/310); however, all tested animals were negative in the conventional PCR (0%, 0/310). Higher amounts of platelets and less cholesterol were detected in seropositive animals but were within the reference values. Ruminal hypomotility and mild tachycardia were observed in all sampled animals. Considering the non-specific clinical signs and the absence of hematological alterations in infected animals, the seropositivity found indicates previous exposure to the protozoan, and the absence of clinically affected animals may result from characteristics inherent to this breed.
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spelling doaj-art-59aa28d9028a41d19d9155046161bb2e2025-08-20T02:18:42ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612025-04-0134110.1590/s1984-29612025019Molecular and serological survey of Trypanosoma vivax in Crioulo Lageano Cattle from southern BrazilFelipe Eduardo FiorinMariana da Silva CasaLeonardo Bergmann GriebelerMariana Fuchs GoedelGianlucca Simão Nadal RibeiroLuiz Flávio Nepomuceno do NascimentoGabriella Bassi das NevesGraziela Vieira FontequeLuiz Cláudio MilettiMere Erika SaitoJoandes Henrique Fontequehttps://orcid.org/0000-0003-1194-6048Abstract Bovine trypanosomiasis, caused by infection with the protozoan Trypanosoma vivax, is harmful to livestock worldwide. Knowing its epidemiology is relevant to evaluate the susceptibility, resistance, and tolerance of animals. The objective of this study was to determine the prevalence of T. vivax in Crioula Lageana cattle and relate them to clinical, hematological, and biochemical findings to elucidate the breed’s health and disease tolerance characteristics. Venous blood samples from 310 bovines considered healthy during the clinical examination were used to perform polymerase chain reaction (PCR) analysis, Immunofluorescence Antibody Test (IFAT) assays, hemogram tests, and serum biochemistry. The collected data were subjected to statistical analyses to compare seropositive and negative groups. IFAT indicated that the seroprevalence for T. vivax was 8% (24/310); however, all tested animals were negative in the conventional PCR (0%, 0/310). Higher amounts of platelets and less cholesterol were detected in seropositive animals but were within the reference values. Ruminal hypomotility and mild tachycardia were observed in all sampled animals. Considering the non-specific clinical signs and the absence of hematological alterations in infected animals, the seropositivity found indicates previous exposure to the protozoan, and the absence of clinically affected animals may result from characteristics inherent to this breed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612025000100406&lng=en&tlng=enLocally adapted breedsanityTrypanosomiasishematologybiochemistry
spellingShingle Felipe Eduardo Fiorin
Mariana da Silva Casa
Leonardo Bergmann Griebeler
Mariana Fuchs Goedel
Gianlucca Simão Nadal Ribeiro
Luiz Flávio Nepomuceno do Nascimento
Gabriella Bassi das Neves
Graziela Vieira Fonteque
Luiz Cláudio Miletti
Mere Erika Saito
Joandes Henrique Fonteque
Molecular and serological survey of Trypanosoma vivax in Crioulo Lageano Cattle from southern Brazil
Revista Brasileira de Parasitologia Veterinária
Locally adapted breed
sanity
Trypanosomiasis
hematology
biochemistry
title Molecular and serological survey of Trypanosoma vivax in Crioulo Lageano Cattle from southern Brazil
title_full Molecular and serological survey of Trypanosoma vivax in Crioulo Lageano Cattle from southern Brazil
title_fullStr Molecular and serological survey of Trypanosoma vivax in Crioulo Lageano Cattle from southern Brazil
title_full_unstemmed Molecular and serological survey of Trypanosoma vivax in Crioulo Lageano Cattle from southern Brazil
title_short Molecular and serological survey of Trypanosoma vivax in Crioulo Lageano Cattle from southern Brazil
title_sort molecular and serological survey of trypanosoma vivax in crioulo lageano cattle from southern brazil
topic Locally adapted breed
sanity
Trypanosomiasis
hematology
biochemistry
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612025000100406&lng=en&tlng=en
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