Multivariate Analyses of Soil Properties and CO<sub>2</sub> Emissions Under Long-Term Fertilization and Crop Rotation in Luvic Chernozem

The key objectives of contemporary agriculture are restoring biodiversity, preserving ecosystem health, reducing the effects of climate change, and producing safe and healthy foods. Maintaining high soil fertility while reducing greenhouse gas emissions requires a precise assessment of how fertiliza...

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Main Authors: Gergana Kuncheva, Galin Gynchev, Jonita Perfanova, Milena Kercheva, Lev Tribis, Hristo Valchovski
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Nitrogen
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Online Access:https://www.mdpi.com/2504-3129/6/2/39
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author Gergana Kuncheva
Galin Gynchev
Jonita Perfanova
Milena Kercheva
Lev Tribis
Hristo Valchovski
author_facet Gergana Kuncheva
Galin Gynchev
Jonita Perfanova
Milena Kercheva
Lev Tribis
Hristo Valchovski
author_sort Gergana Kuncheva
collection DOAJ
description The key objectives of contemporary agriculture are restoring biodiversity, preserving ecosystem health, reducing the effects of climate change, and producing safe and healthy foods. Maintaining high soil fertility while reducing greenhouse gas emissions requires a precise assessment of how fertilization and crop rotation affect carbon and nutrient cycles in agroecosystems. Fertilization affects soil conditions, which alters the environment for soil microbial development and influences the number and composition of soil microbial communities, leading to changes in nutrient and carbon cycling. There is a lack of long-term experimental data on the impact of fertilizer treatments on soil CO<sub>2</sub> emissions, soil microbial communities, and their interactions. The novelty of this study is that it identified the fertilization effects on soil carbon sequestration, soil properties, and microbial communities in the context of a long-term fertilizer experiment in Luvic Chernozem. The fertilization treatments that were continuously pplied for 64 years under a four-crop (wheat, barley, corn, and bean) rotation were nitrogen (N), phosphorus (P), potassium (K), NP, NK, PK, NPK, and control. The chemical and microbiological soil properties and soil CO<sub>2</sub> emissions were monitored. The highest organic carbon content was observed under the NPK (1.42%) and NP (1.43%) treatments. N fertilizer application most significantly affected soil properties, including pH, electrical conductivity, and soil organic carbon content, altering the environment for soil microbial development and influencing the number and composition of soil microbial communities. On average, the field-measured soil C-CO<sub>2</sub> emissions were the most intensive under NP (2.76 kg ha<sup>−1</sup> h<sup>−1</sup>), NPK (2.83 kg ha<sup>−1</sup> h<sup>−1</sup>), and PK (2.51 kg ha<sup>−1</sup> day<sup>−1</sup>) treatments.
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spelling doaj-art-59a6c1d8dd434b5287cd72ac353d8c0f2025-08-20T03:16:35ZengMDPI AGNitrogen2504-31292025-05-01623910.3390/nitrogen6020039Multivariate Analyses of Soil Properties and CO<sub>2</sub> Emissions Under Long-Term Fertilization and Crop Rotation in Luvic ChernozemGergana Kuncheva0Galin Gynchev1Jonita Perfanova2Milena Kercheva3Lev Tribis4Hristo Valchovski5Institute of Soil Science, Agrotechnologies and Plant Protection “Nikola Poushkarov”, Agricultural Academy, 1331 Sofia, BulgariaIASS “Obraztsov Chiflik”, Agricultural Academy, 7007 Ruse, BulgariaInstitute of Soil Science, Agrotechnologies and Plant Protection “Nikola Poushkarov”, Agricultural Academy, 1331 Sofia, BulgariaInstitute of Soil Science, Agrotechnologies and Plant Protection “Nikola Poushkarov”, Agricultural Academy, 1331 Sofia, BulgariaInstitute of Soil Science, Agrotechnologies and Plant Protection “Nikola Poushkarov”, Agricultural Academy, 1331 Sofia, BulgariaInstitute of Soil Science, Agrotechnologies and Plant Protection “Nikola Poushkarov”, Agricultural Academy, 1331 Sofia, BulgariaThe key objectives of contemporary agriculture are restoring biodiversity, preserving ecosystem health, reducing the effects of climate change, and producing safe and healthy foods. Maintaining high soil fertility while reducing greenhouse gas emissions requires a precise assessment of how fertilization and crop rotation affect carbon and nutrient cycles in agroecosystems. Fertilization affects soil conditions, which alters the environment for soil microbial development and influences the number and composition of soil microbial communities, leading to changes in nutrient and carbon cycling. There is a lack of long-term experimental data on the impact of fertilizer treatments on soil CO<sub>2</sub> emissions, soil microbial communities, and their interactions. The novelty of this study is that it identified the fertilization effects on soil carbon sequestration, soil properties, and microbial communities in the context of a long-term fertilizer experiment in Luvic Chernozem. The fertilization treatments that were continuously pplied for 64 years under a four-crop (wheat, barley, corn, and bean) rotation were nitrogen (N), phosphorus (P), potassium (K), NP, NK, PK, NPK, and control. The chemical and microbiological soil properties and soil CO<sub>2</sub> emissions were monitored. The highest organic carbon content was observed under the NPK (1.42%) and NP (1.43%) treatments. N fertilizer application most significantly affected soil properties, including pH, electrical conductivity, and soil organic carbon content, altering the environment for soil microbial development and influencing the number and composition of soil microbial communities. On average, the field-measured soil C-CO<sub>2</sub> emissions were the most intensive under NP (2.76 kg ha<sup>−1</sup> h<sup>−1</sup>), NPK (2.83 kg ha<sup>−1</sup> h<sup>−1</sup>), and PK (2.51 kg ha<sup>−1</sup> day<sup>−1</sup>) treatments.https://www.mdpi.com/2504-3129/6/2/39long-term fertilizer experimentfertilizationsoil organic carbonsoil microbiotasoil CO<sub>2</sub> emissions
spellingShingle Gergana Kuncheva
Galin Gynchev
Jonita Perfanova
Milena Kercheva
Lev Tribis
Hristo Valchovski
Multivariate Analyses of Soil Properties and CO<sub>2</sub> Emissions Under Long-Term Fertilization and Crop Rotation in Luvic Chernozem
Nitrogen
long-term fertilizer experiment
fertilization
soil organic carbon
soil microbiota
soil CO<sub>2</sub> emissions
title Multivariate Analyses of Soil Properties and CO<sub>2</sub> Emissions Under Long-Term Fertilization and Crop Rotation in Luvic Chernozem
title_full Multivariate Analyses of Soil Properties and CO<sub>2</sub> Emissions Under Long-Term Fertilization and Crop Rotation in Luvic Chernozem
title_fullStr Multivariate Analyses of Soil Properties and CO<sub>2</sub> Emissions Under Long-Term Fertilization and Crop Rotation in Luvic Chernozem
title_full_unstemmed Multivariate Analyses of Soil Properties and CO<sub>2</sub> Emissions Under Long-Term Fertilization and Crop Rotation in Luvic Chernozem
title_short Multivariate Analyses of Soil Properties and CO<sub>2</sub> Emissions Under Long-Term Fertilization and Crop Rotation in Luvic Chernozem
title_sort multivariate analyses of soil properties and co sub 2 sub emissions under long term fertilization and crop rotation in luvic chernozem
topic long-term fertilizer experiment
fertilization
soil organic carbon
soil microbiota
soil CO<sub>2</sub> emissions
url https://www.mdpi.com/2504-3129/6/2/39
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AT jonitaperfanova multivariateanalysesofsoilpropertiesandcosub2subemissionsunderlongtermfertilizationandcroprotationinluvicchernozem
AT milenakercheva multivariateanalysesofsoilpropertiesandcosub2subemissionsunderlongtermfertilizationandcroprotationinluvicchernozem
AT levtribis multivariateanalysesofsoilpropertiesandcosub2subemissionsunderlongtermfertilizationandcroprotationinluvicchernozem
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