Efficacy of Rapid Salivary C-Reactive Protein Test to Assess Early Changes in Malignancy in the Oral Cavity and Its Utility in Screening for Oral Cancer

<b>Background/Objectives:</b> The present study aimed to test the efficacy of the chair-side rapid salivary C-reactive protein assay kit in differentiating oral potentially malignant disorders (OPMDs) and oral cancer from normal mucosa using whole salivary samples. <b>Methods:</...

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Main Authors: Vathsala Patil, Ravindranath Vineetha, Komal Smriti, Kalyana Chakravarthy Pentapati, Srikanth Gadicherla, Carnelio Sunitha
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Dentistry Journal
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Online Access:https://www.mdpi.com/2304-6767/13/1/34
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author Vathsala Patil
Ravindranath Vineetha
Komal Smriti
Kalyana Chakravarthy Pentapati
Srikanth Gadicherla
Carnelio Sunitha
author_facet Vathsala Patil
Ravindranath Vineetha
Komal Smriti
Kalyana Chakravarthy Pentapati
Srikanth Gadicherla
Carnelio Sunitha
author_sort Vathsala Patil
collection DOAJ
description <b>Background/Objectives:</b> The present study aimed to test the efficacy of the chair-side rapid salivary C-reactive protein assay kit in differentiating oral potentially malignant disorders (OPMDs) and oral cancer from normal mucosa using whole salivary samples. <b>Methods:</b> In this study, unstimulated saliva samples of cases (OPMDs and oral cancer) and controls (systemically healthy subjects) were used to detect CRP levels using a novel colorimetric, quantitative rapid assay kit. Kruskal–Wallis ANOVA with a post hoc Dunn’s test were applied to determine the difference in the mean and SD values between the case and control groups. ROC analysis was performed to identify the positive and negative likelihood ratios. <b>Results:</b> The mean calculated salivary CRP level in the oral cancer group was 4.21 ng/mL, in the OPMD group it was 2.51 ng/mL and in the healthy controls it was 0.7 ng/mL. Post hoc tests showed that the salivary CRP levels were significantly higher in the oral cancer and OPMD groups than in the controls. <b>Conclusions:</b> The present study showed that the novel rapid salivary test kit could significantly differentiate between the salivary CRP values of cases and controls but there was no significant difference between the OPMD and malignancy groups. It also showed higher sensitivity values, confirming the efficacy of this kit as a screening tool.
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spelling doaj-art-59a31ec9959143c286531090b7fd75e02025-01-24T13:28:42ZengMDPI AGDentistry Journal2304-67672025-01-011313410.3390/dj13010034Efficacy of Rapid Salivary C-Reactive Protein Test to Assess Early Changes in Malignancy in the Oral Cavity and Its Utility in Screening for Oral CancerVathsala Patil0Ravindranath Vineetha1Komal Smriti2Kalyana Chakravarthy Pentapati3Srikanth Gadicherla4Carnelio Sunitha5Department of Oral Medicine & Radiology, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Udupi 576104, Karnataka, IndiaDepartment of Oral Medicine & Radiology, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Udupi 576104, Karnataka, IndiaDepartment of Oral Medicine & Radiology, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Udupi 576104, Karnataka, IndiaDepartment of Public Health Dentistry, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Udupi 576104, Karnataka, IndiaDepartment of Oral and Maxillofacial Surgery, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Udupi 576104, Karnataka, IndiaDepartment of Oral Pathology and Microbiology, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Udupi 576104, Karnataka, India<b>Background/Objectives:</b> The present study aimed to test the efficacy of the chair-side rapid salivary C-reactive protein assay kit in differentiating oral potentially malignant disorders (OPMDs) and oral cancer from normal mucosa using whole salivary samples. <b>Methods:</b> In this study, unstimulated saliva samples of cases (OPMDs and oral cancer) and controls (systemically healthy subjects) were used to detect CRP levels using a novel colorimetric, quantitative rapid assay kit. Kruskal–Wallis ANOVA with a post hoc Dunn’s test were applied to determine the difference in the mean and SD values between the case and control groups. ROC analysis was performed to identify the positive and negative likelihood ratios. <b>Results:</b> The mean calculated salivary CRP level in the oral cancer group was 4.21 ng/mL, in the OPMD group it was 2.51 ng/mL and in the healthy controls it was 0.7 ng/mL. Post hoc tests showed that the salivary CRP levels were significantly higher in the oral cancer and OPMD groups than in the controls. <b>Conclusions:</b> The present study showed that the novel rapid salivary test kit could significantly differentiate between the salivary CRP values of cases and controls but there was no significant difference between the OPMD and malignancy groups. It also showed higher sensitivity values, confirming the efficacy of this kit as a screening tool.https://www.mdpi.com/2304-6767/13/1/34oral cancerC-reactive proteinoral potentially malignant disorderbiomarkersaliva
spellingShingle Vathsala Patil
Ravindranath Vineetha
Komal Smriti
Kalyana Chakravarthy Pentapati
Srikanth Gadicherla
Carnelio Sunitha
Efficacy of Rapid Salivary C-Reactive Protein Test to Assess Early Changes in Malignancy in the Oral Cavity and Its Utility in Screening for Oral Cancer
Dentistry Journal
oral cancer
C-reactive protein
oral potentially malignant disorder
biomarker
saliva
title Efficacy of Rapid Salivary C-Reactive Protein Test to Assess Early Changes in Malignancy in the Oral Cavity and Its Utility in Screening for Oral Cancer
title_full Efficacy of Rapid Salivary C-Reactive Protein Test to Assess Early Changes in Malignancy in the Oral Cavity and Its Utility in Screening for Oral Cancer
title_fullStr Efficacy of Rapid Salivary C-Reactive Protein Test to Assess Early Changes in Malignancy in the Oral Cavity and Its Utility in Screening for Oral Cancer
title_full_unstemmed Efficacy of Rapid Salivary C-Reactive Protein Test to Assess Early Changes in Malignancy in the Oral Cavity and Its Utility in Screening for Oral Cancer
title_short Efficacy of Rapid Salivary C-Reactive Protein Test to Assess Early Changes in Malignancy in the Oral Cavity and Its Utility in Screening for Oral Cancer
title_sort efficacy of rapid salivary c reactive protein test to assess early changes in malignancy in the oral cavity and its utility in screening for oral cancer
topic oral cancer
C-reactive protein
oral potentially malignant disorder
biomarker
saliva
url https://www.mdpi.com/2304-6767/13/1/34
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