Weathered antlers as a source of DNA
Abstract We tested antlers of Coues white‐tailed (Odocoileus virginianus couesi) and mule deer (O. hemionus) in various stages of natural decomposition to determine the degree of weathering that cast antlers could endure and still yield usable DNA. Based on physical characteristics, we partitioned a...
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| Format: | Article |
| Language: | English |
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Wiley
2012-06-01
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| Series: | Wildlife Society Bulletin |
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| Online Access: | https://doi.org/10.1002/wsb.138 |
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| author | Roy G. Lopez Paul Beier |
| author_facet | Roy G. Lopez Paul Beier |
| author_sort | Roy G. Lopez |
| collection | DOAJ |
| description | Abstract We tested antlers of Coues white‐tailed (Odocoileus virginianus couesi) and mule deer (O. hemionus) in various stages of natural decomposition to determine the degree of weathering that cast antlers could endure and still yield usable DNA. Based on physical characteristics, we partitioned antlers into 7 weathering categories ranging from freshly cast (class 1) to having been exposed to weathering for ≥8 years (class 7). Most antlers that had weathered 1–7 years yielded amplifiable DNA, whereas antlers weathered for ≥8 years failed to yield replicable DNA. This study confirms that antlers exposed to weathering for 1–7 years can be a source of replicable DNA and, thus, contribute an additional, and previously overlooked, non‐invasive sample for analysis in molecular genetics studies. Published 2012. This article is a U.S. Government work and is in the public domain in the USA. |
| format | Article |
| id | doaj-art-598fa5e9da3a4bd7b38099396c9a34cd |
| institution | Kabale University |
| issn | 2328-5540 |
| language | English |
| publishDate | 2012-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Wildlife Society Bulletin |
| spelling | doaj-art-598fa5e9da3a4bd7b38099396c9a34cd2024-12-16T11:25:55ZengWileyWildlife Society Bulletin2328-55402012-06-0136238038210.1002/wsb.138Weathered antlers as a source of DNARoy G. Lopez0Paul Beier1Rocky Mountain Research Station, 2500 S Pine Knoll Drive, Flagstaff, AZ 86001, USANorthern Arizona University, School of Forestry, 200 E Pine Knoll Drive, Flagstaff, AZ 86001, USAAbstract We tested antlers of Coues white‐tailed (Odocoileus virginianus couesi) and mule deer (O. hemionus) in various stages of natural decomposition to determine the degree of weathering that cast antlers could endure and still yield usable DNA. Based on physical characteristics, we partitioned antlers into 7 weathering categories ranging from freshly cast (class 1) to having been exposed to weathering for ≥8 years (class 7). Most antlers that had weathered 1–7 years yielded amplifiable DNA, whereas antlers weathered for ≥8 years failed to yield replicable DNA. This study confirms that antlers exposed to weathering for 1–7 years can be a source of replicable DNA and, thus, contribute an additional, and previously overlooked, non‐invasive sample for analysis in molecular genetics studies. Published 2012. This article is a U.S. Government work and is in the public domain in the USA.https://doi.org/10.1002/wsb.138antlersCoues white‐tailed deerDNAmule deerOdocoileus hemionusOdocoileus virginianus couesi |
| spellingShingle | Roy G. Lopez Paul Beier Weathered antlers as a source of DNA Wildlife Society Bulletin antlers Coues white‐tailed deer DNA mule deer Odocoileus hemionus Odocoileus virginianus couesi |
| title | Weathered antlers as a source of DNA |
| title_full | Weathered antlers as a source of DNA |
| title_fullStr | Weathered antlers as a source of DNA |
| title_full_unstemmed | Weathered antlers as a source of DNA |
| title_short | Weathered antlers as a source of DNA |
| title_sort | weathered antlers as a source of dna |
| topic | antlers Coues white‐tailed deer DNA mule deer Odocoileus hemionus Odocoileus virginianus couesi |
| url | https://doi.org/10.1002/wsb.138 |
| work_keys_str_mv | AT royglopez weatheredantlersasasourceofdna AT paulbeier weatheredantlersasasourceofdna |