Kiperin Double-Hydrolyzed Collagen as a Potential Anti-Tumor Agent: Effects on HCT116 Colon Carcinoma Cells and Oxidative Stress Modulation
Double-hydrolyzed collagen, a key structural protein, has gained increasing attention for its role in cancer progression and its potential therapeutic applications. This study aims to investigate the effects of double-hydrolyzed collagen (Type I and III peptides) on HCT116 colon carcinoma cells and...
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2025-05-01
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| author | Lutfiye Karcioglu Batur Cuneyd Yavas Nezih Hekim |
| author_facet | Lutfiye Karcioglu Batur Cuneyd Yavas Nezih Hekim |
| author_sort | Lutfiye Karcioglu Batur |
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| description | Double-hydrolyzed collagen, a key structural protein, has gained increasing attention for its role in cancer progression and its potential therapeutic applications. This study aims to investigate the effects of double-hydrolyzed collagen (Type I and III peptides) on HCT116 colon carcinoma cells and CCD-18Co fibroblasts as a normal control. Cells were treated with 0.5 µg/mL, 1 µg/mL, and 1.5 µg/mL of collagen peptide solution. HCT116 and CCD-18Co cells were cultured under standard conditions and treated with 1 µg/mL collagen. Cell viability (MTT assay), migration (scratch assay), oxidative stress (TAS/TOS kits), <i>TNF-α</i> expression (qRT-PCR), and tumor marker levels (CA19-9, CEA, CA72-4, and CYFRA 21-1; CLIA) were evaluated. Cell viability, proliferation, migration, oxidative stress, and tumor marker levels were assessed. Statistical analyses were performed to determine significance. Double-hydrolyzed collagen treatment significantly increased CCD-18Co fibroblast proliferation (<i>p</i> = 0.0143), while HCT116 cancer cell numbers significantly decreased (<i>p</i> = 0.0045). Migration of HCT116 cells was markedly reduced (<i>p</i> < 0.0001), whereas no significant effect was observed in CCD-18Co fibroblasts (<i>p</i> = 0.559). Oxidative stress analysis showed decreased total oxidative status (TOS) and increased total antioxidant status (TAS) in HCT116 cells (<i>p</i> = 0.0075 and <i>p</i> = 0.0095, respectively), with no significant changes in normal fibroblasts. Among tumor markers, CA19-9 levels were significantly reduced in HCT116 cells (<i>p</i> = 0.013), while CEA, CA72-4, and CYFRA 21-1 remained unchanged. <i>TNF-α</i> gene expression analysis confirmed the absence of inflammatory or adverse effects in normal fibroblasts. These findings suggest that double-hydrolyzed collagen selectively inhibits colon cancer cell proliferation and migration, modulates oxidative stress, and reduces CA19-9 levels while promoting fibroblast growth. The differential effects between cancerous and normal cells highlight collagen’s potential as a complementary therapeutic approach for colorectal cancer. Further research is needed to elucidate the underlying mechanisms and assess its clinical applicability. Double-hydrolyzed collagen appears to be a safe and beneficial dietary component with promising biological effects and therapeutic potential. |
| format | Article |
| id | doaj-art-597b8ebc7e394342a786e1adccc2cb18 |
| institution | DOAJ |
| issn | 1467-3037 1467-3045 |
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| spelling | doaj-art-597b8ebc7e394342a786e1adccc2cb182025-08-20T03:14:42ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-05-0147536410.3390/cimb47050364Kiperin Double-Hydrolyzed Collagen as a Potential Anti-Tumor Agent: Effects on HCT116 Colon Carcinoma Cells and Oxidative Stress ModulationLutfiye Karcioglu Batur0Cuneyd Yavas1Nezih Hekim2Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Biruni University, Merkezefendi, 75 Sk No:1-13 M.G., 34015 Istanbul, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Biruni University, Merkezefendi, 75 Sk No:1-13 M.G., 34015 Istanbul, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Biruni University, Merkezefendi, 75 Sk No:1-13 M.G., 34015 Istanbul, TurkeyDouble-hydrolyzed collagen, a key structural protein, has gained increasing attention for its role in cancer progression and its potential therapeutic applications. This study aims to investigate the effects of double-hydrolyzed collagen (Type I and III peptides) on HCT116 colon carcinoma cells and CCD-18Co fibroblasts as a normal control. Cells were treated with 0.5 µg/mL, 1 µg/mL, and 1.5 µg/mL of collagen peptide solution. HCT116 and CCD-18Co cells were cultured under standard conditions and treated with 1 µg/mL collagen. Cell viability (MTT assay), migration (scratch assay), oxidative stress (TAS/TOS kits), <i>TNF-α</i> expression (qRT-PCR), and tumor marker levels (CA19-9, CEA, CA72-4, and CYFRA 21-1; CLIA) were evaluated. Cell viability, proliferation, migration, oxidative stress, and tumor marker levels were assessed. Statistical analyses were performed to determine significance. Double-hydrolyzed collagen treatment significantly increased CCD-18Co fibroblast proliferation (<i>p</i> = 0.0143), while HCT116 cancer cell numbers significantly decreased (<i>p</i> = 0.0045). Migration of HCT116 cells was markedly reduced (<i>p</i> < 0.0001), whereas no significant effect was observed in CCD-18Co fibroblasts (<i>p</i> = 0.559). Oxidative stress analysis showed decreased total oxidative status (TOS) and increased total antioxidant status (TAS) in HCT116 cells (<i>p</i> = 0.0075 and <i>p</i> = 0.0095, respectively), with no significant changes in normal fibroblasts. Among tumor markers, CA19-9 levels were significantly reduced in HCT116 cells (<i>p</i> = 0.013), while CEA, CA72-4, and CYFRA 21-1 remained unchanged. <i>TNF-α</i> gene expression analysis confirmed the absence of inflammatory or adverse effects in normal fibroblasts. These findings suggest that double-hydrolyzed collagen selectively inhibits colon cancer cell proliferation and migration, modulates oxidative stress, and reduces CA19-9 levels while promoting fibroblast growth. The differential effects between cancerous and normal cells highlight collagen’s potential as a complementary therapeutic approach for colorectal cancer. Further research is needed to elucidate the underlying mechanisms and assess its clinical applicability. Double-hydrolyzed collagen appears to be a safe and beneficial dietary component with promising biological effects and therapeutic potential.https://www.mdpi.com/1467-3045/47/5/364double-hydrolyzed collagenHCT116 colon carcinomacell viabilitycell migrationoxidative stresstotal antioxidant status |
| spellingShingle | Lutfiye Karcioglu Batur Cuneyd Yavas Nezih Hekim Kiperin Double-Hydrolyzed Collagen as a Potential Anti-Tumor Agent: Effects on HCT116 Colon Carcinoma Cells and Oxidative Stress Modulation Current Issues in Molecular Biology double-hydrolyzed collagen HCT116 colon carcinoma cell viability cell migration oxidative stress total antioxidant status |
| title | Kiperin Double-Hydrolyzed Collagen as a Potential Anti-Tumor Agent: Effects on HCT116 Colon Carcinoma Cells and Oxidative Stress Modulation |
| title_full | Kiperin Double-Hydrolyzed Collagen as a Potential Anti-Tumor Agent: Effects on HCT116 Colon Carcinoma Cells and Oxidative Stress Modulation |
| title_fullStr | Kiperin Double-Hydrolyzed Collagen as a Potential Anti-Tumor Agent: Effects on HCT116 Colon Carcinoma Cells and Oxidative Stress Modulation |
| title_full_unstemmed | Kiperin Double-Hydrolyzed Collagen as a Potential Anti-Tumor Agent: Effects on HCT116 Colon Carcinoma Cells and Oxidative Stress Modulation |
| title_short | Kiperin Double-Hydrolyzed Collagen as a Potential Anti-Tumor Agent: Effects on HCT116 Colon Carcinoma Cells and Oxidative Stress Modulation |
| title_sort | kiperin double hydrolyzed collagen as a potential anti tumor agent effects on hct116 colon carcinoma cells and oxidative stress modulation |
| topic | double-hydrolyzed collagen HCT116 colon carcinoma cell viability cell migration oxidative stress total antioxidant status |
| url | https://www.mdpi.com/1467-3045/47/5/364 |
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