451 Engineered multifunctional wound healing patch: an antimicrobial living bandage to improve wound healing
Objectives/Goals: The study focuses on developing a wound patch that employs a biocompatible matrix which incorporates mesenchymal stem cells (MSCs) with wound healing and antimicrobial properties, along with antimicrobial metallic nanoparticles covered with keratinocytes derived from induced plurip...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cambridge University Press
2025-04-01
|
| Series: | Journal of Clinical and Translational Science |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2059866124010483/type/journal_article |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849393749750185984 |
|---|---|
| author | Valerie Johnson Nureddin Ashammakhi Morteza Mahmoudi |
| author_facet | Valerie Johnson Nureddin Ashammakhi Morteza Mahmoudi |
| author_sort | Valerie Johnson |
| collection | DOAJ |
| description | Objectives/Goals: The study focuses on developing a wound patch that employs a biocompatible matrix which incorporates mesenchymal stem cells (MSCs) with wound healing and antimicrobial properties, along with antimicrobial metallic nanoparticles covered with keratinocytes derived from induced pluripotent stem cells to replicate the skin’s barrier function. Methods/Study Population: In vitro experiments will be conducted to combine bacteria with MSCs and metallic nanoparticles to assess whether bacterial killing is improved by this combination. The MSCs will then be evaluated in the presence of the nanoparticles to confirm that their functionality and phenotype are not altered. To verify the cells’ functional integrity, they will undergo trilineage differentiation, surface marker phenotypic testing, and evaluation of their capacity to inhibit lymphocyte proliferation in the presence of the nanoparticles. Subsequently, this living bandage will be created using a biomatrix embedded with induced pluripotent stem cell-derived keratinocytes and tested on a canine wound model to study the impact on healing. The model will assess the rate of healing and cellular response at weekly intervals until healed. Results/Anticipated Results: The combination of mesenchymal stem cells and antimicrobial nanoparticles works synergistically to enhance bacterial killing in vitro with S. aureus. The presence of the nanoparticles in combination with MSC did not affect the ability of the MSC to undergo trilineage differentiation. We anticipate that the surface phenotype will be similarly unaffected. In addition, we expect that the presence of the nanoparticles should not interfere with the ability of MSC to suppress lymphocyte proliferation. Utilization of the wound patch in the in vivo canine wound model is expected to enhance healing and prevent infection. We expect that we will observe a shift in the cellular composition of the wound with less inflammatory cells and more M2 or wound healing anti-inflammatory monocytes. Discussion/Significance of Impact: The incidence of resistant infections with no pharmacologic therapy available are on the rise. The development of an antimicrobial living bandage that increases the body’s ability to fight off infection, while providing a barrier to reinfection would provide a new way to treat infections regardless of their acquired antibacterial resistance. |
| format | Article |
| id | doaj-art-597ad583f77545a0b128e3d5bdb4fd8a |
| institution | Kabale University |
| issn | 2059-8661 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj-art-597ad583f77545a0b128e3d5bdb4fd8a2025-08-20T03:40:18ZengCambridge University PressJournal of Clinical and Translational Science2059-86612025-04-01913313310.1017/cts.2024.1048451 Engineered multifunctional wound healing patch: an antimicrobial living bandage to improve wound healingValerie Johnson0Nureddin Ashammakhi1Morteza Mahmoudi2Michigan State UniversityMichigan State UniversityMichigan State UniversityObjectives/Goals: The study focuses on developing a wound patch that employs a biocompatible matrix which incorporates mesenchymal stem cells (MSCs) with wound healing and antimicrobial properties, along with antimicrobial metallic nanoparticles covered with keratinocytes derived from induced pluripotent stem cells to replicate the skin’s barrier function. Methods/Study Population: In vitro experiments will be conducted to combine bacteria with MSCs and metallic nanoparticles to assess whether bacterial killing is improved by this combination. The MSCs will then be evaluated in the presence of the nanoparticles to confirm that their functionality and phenotype are not altered. To verify the cells’ functional integrity, they will undergo trilineage differentiation, surface marker phenotypic testing, and evaluation of their capacity to inhibit lymphocyte proliferation in the presence of the nanoparticles. Subsequently, this living bandage will be created using a biomatrix embedded with induced pluripotent stem cell-derived keratinocytes and tested on a canine wound model to study the impact on healing. The model will assess the rate of healing and cellular response at weekly intervals until healed. Results/Anticipated Results: The combination of mesenchymal stem cells and antimicrobial nanoparticles works synergistically to enhance bacterial killing in vitro with S. aureus. The presence of the nanoparticles in combination with MSC did not affect the ability of the MSC to undergo trilineage differentiation. We anticipate that the surface phenotype will be similarly unaffected. In addition, we expect that the presence of the nanoparticles should not interfere with the ability of MSC to suppress lymphocyte proliferation. Utilization of the wound patch in the in vivo canine wound model is expected to enhance healing and prevent infection. We expect that we will observe a shift in the cellular composition of the wound with less inflammatory cells and more M2 or wound healing anti-inflammatory monocytes. Discussion/Significance of Impact: The incidence of resistant infections with no pharmacologic therapy available are on the rise. The development of an antimicrobial living bandage that increases the body’s ability to fight off infection, while providing a barrier to reinfection would provide a new way to treat infections regardless of their acquired antibacterial resistance.https://www.cambridge.org/core/product/identifier/S2059866124010483/type/journal_article |
| spellingShingle | Valerie Johnson Nureddin Ashammakhi Morteza Mahmoudi 451 Engineered multifunctional wound healing patch: an antimicrobial living bandage to improve wound healing Journal of Clinical and Translational Science |
| title | 451 Engineered multifunctional wound healing patch: an antimicrobial living bandage to improve wound healing |
| title_full | 451 Engineered multifunctional wound healing patch: an antimicrobial living bandage to improve wound healing |
| title_fullStr | 451 Engineered multifunctional wound healing patch: an antimicrobial living bandage to improve wound healing |
| title_full_unstemmed | 451 Engineered multifunctional wound healing patch: an antimicrobial living bandage to improve wound healing |
| title_short | 451 Engineered multifunctional wound healing patch: an antimicrobial living bandage to improve wound healing |
| title_sort | 451 engineered multifunctional wound healing patch an antimicrobial living bandage to improve wound healing |
| url | https://www.cambridge.org/core/product/identifier/S2059866124010483/type/journal_article |
| work_keys_str_mv | AT valeriejohnson 451engineeredmultifunctionalwoundhealingpatchanantimicrobiallivingbandagetoimprovewoundhealing AT nureddinashammakhi 451engineeredmultifunctionalwoundhealingpatchanantimicrobiallivingbandagetoimprovewoundhealing AT mortezamahmoudi 451engineeredmultifunctionalwoundhealingpatchanantimicrobiallivingbandagetoimprovewoundhealing |