Exploration of the antitumor activity and related mechanisms of RAD5 on colon cancer HT29 Cells
[Objectives] To investigate the antitumor activity and potential mechanisms of rosmarinic acid derivative 5 (RAD5) on colon cancer HT29 cells. [Methods] Colon cancer HT29 cells were treated with different concentrations of RAD5 (0 μmol/L, 12.5 μmol/L, 25 μmol/L, 50 μmol/L, 100 μmol/L, 150 μmol/L) fo...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Colorectal & Anal Surgery
2024-06-01
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| Series: | 结直肠肛门外科 |
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| Online Access: | https://jcas.gxmuyfy.cn/cn/wqll/paper.html?id=276&cateName=2024%E5%B9%B4%20%E7%AC%AC30%E5%8D%B7%20%E7%AC%AC3%E6%9C%9F |
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| author | Wei Liqun Xu Chengfei Pan Xiaohang Ruan Guotian Yan Ling Tang Shuangyi Gan Jialiang |
| author_facet | Wei Liqun Xu Chengfei Pan Xiaohang Ruan Guotian Yan Ling Tang Shuangyi Gan Jialiang |
| author_sort | Wei Liqun |
| collection | DOAJ |
| description | [Objectives] To investigate the antitumor activity and potential mechanisms of rosmarinic acid derivative 5 (RAD5) on colon cancer HT29 cells. [Methods] Colon cancer HT29 cells were treated with different concentrations of RAD5 (0 μmol/L, 12.5 μmol/L, 25 μmol/L, 50 μmol/L, 100 μmol/L, 150 μmol/L) for 36 hours, and cell viability was detected using the MTT assay. Colon cancer HT29 cells were divided into 3 groups and treated with RAD5 for 36 hours with 0 μmol/L, 25 μmol/L and 50 μmol/L respectively, the cell proliferation ability was assessed using a colony formation assay, apoptosis rate was detected by Hoechst 33258 staining and flow cytometry, and the expression of apoptosis-related proteins Bax, Bcl-2, and EGFR/MAPK pathway-related proteins was examined by Western blot. [Results] The results of cell experiments showed that RAD5 could significantly inhibit the proliferation of colon cancer HT29 cells in a concentration-dependent manner. Compared with the control group (0 μmol/L), the clone formation rates of the 25 μmol/L and 50 μmol/L groups were significantly reduced (P<0.001), apoptosis rates increased (P<0.01), Bax expression increased, Bcl-2 expression showed a downward trend, and the expression of EGFR and ERK1/2 proteins in the EGFR/MAPK pathway decreased, while the ratios of p-ERK1/2/ERK1/2 and p-P38/P38 were elevated (P<0.001). [Conclusion] RAD5 exhibits antitumor activity against colon cancer HT29 cell, and its mechanism may be related to inhibiting EGFR expression and activating the phosphorylation of ERK1/2 and P38 in the downstream MAPK pathway, providing experimental theoretical support for its development as an anticancer candidate drug. |
| format | Article |
| id | doaj-art-5979e62138ca4103b38d790730e1bfff |
| institution | Kabale University |
| issn | 1674-0491 |
| language | zho |
| publishDate | 2024-06-01 |
| publisher | Editorial Office of Journal of Colorectal & Anal Surgery |
| record_format | Article |
| series | 结直肠肛门外科 |
| spelling | doaj-art-5979e62138ca4103b38d790730e1bfff2025-08-20T03:49:17ZzhoEditorial Office of Journal of Colorectal & Anal Surgery结直肠肛门外科1674-04912024-06-0130332232810.19668/j.cnki.issn1674-0491.2024.03.014Exploration of the antitumor activity and related mechanisms of RAD5 on colon cancer HT29 CellsWei Liqun0Xu Chengfei1Pan Xiaohang2Ruan Guotian3Yan Ling4Tang Shuangyi5Gan Jialiang6Department of Pharmacy, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaColorectal ＆ Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaColorectal ＆ Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaColorectal ＆ Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaColorectal ＆ Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaDepartment of Pharmacy, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaColorectal ＆ Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China[Objectives] To investigate the antitumor activity and potential mechanisms of rosmarinic acid derivative 5 (RAD5) on colon cancer HT29 cells. [Methods] Colon cancer HT29 cells were treated with different concentrations of RAD5 (0 μmol/L, 12.5 μmol/L, 25 μmol/L, 50 μmol/L, 100 μmol/L, 150 μmol/L) for 36 hours, and cell viability was detected using the MTT assay. Colon cancer HT29 cells were divided into 3 groups and treated with RAD5 for 36 hours with 0 μmol/L, 25 μmol/L and 50 μmol/L respectively, the cell proliferation ability was assessed using a colony formation assay, apoptosis rate was detected by Hoechst 33258 staining and flow cytometry, and the expression of apoptosis-related proteins Bax, Bcl-2, and EGFR/MAPK pathway-related proteins was examined by Western blot. [Results] The results of cell experiments showed that RAD5 could significantly inhibit the proliferation of colon cancer HT29 cells in a concentration-dependent manner. Compared with the control group (0 μmol/L), the clone formation rates of the 25 μmol/L and 50 μmol/L groups were significantly reduced (P<0.001), apoptosis rates increased (P<0.01), Bax expression increased, Bcl-2 expression showed a downward trend, and the expression of EGFR and ERK1/2 proteins in the EGFR/MAPK pathway decreased, while the ratios of p-ERK1/2/ERK1/2 and p-P38/P38 were elevated (P<0.001). [Conclusion] RAD5 exhibits antitumor activity against colon cancer HT29 cell, and its mechanism may be related to inhibiting EGFR expression and activating the phosphorylation of ERK1/2 and P38 in the downstream MAPK pathway, providing experimental theoretical support for its development as an anticancer candidate drug.https://jcas.gxmuyfy.cn/cn/wqll/paper.html?id=276&cateName=2024%E5%B9%B4%20%E7%AC%AC30%E5%8D%B7%20%E7%AC%AC3%E6%9C%9Fcolon cancerrosmarinic acid derivativeproliferationapoptosismechanism |
| spellingShingle | Wei Liqun Xu Chengfei Pan Xiaohang Ruan Guotian Yan Ling Tang Shuangyi Gan Jialiang Exploration of the antitumor activity and related mechanisms of RAD5 on colon cancer HT29 Cells 结直肠肛门外科 colon cancer rosmarinic acid derivative proliferation apoptosis mechanism |
| title | Exploration of the antitumor activity and related mechanisms of RAD5 on colon cancer HT29 Cells |
| title_full | Exploration of the antitumor activity and related mechanisms of RAD5 on colon cancer HT29 Cells |
| title_fullStr | Exploration of the antitumor activity and related mechanisms of RAD5 on colon cancer HT29 Cells |
| title_full_unstemmed | Exploration of the antitumor activity and related mechanisms of RAD5 on colon cancer HT29 Cells |
| title_short | Exploration of the antitumor activity and related mechanisms of RAD5 on colon cancer HT29 Cells |
| title_sort | exploration of the antitumor activity and related mechanisms of rad5 on colon cancer ht29 cells |
| topic | colon cancer rosmarinic acid derivative proliferation apoptosis mechanism |
| url | https://jcas.gxmuyfy.cn/cn/wqll/paper.html?id=276&cateName=2024%E5%B9%B4%20%E7%AC%AC30%E5%8D%B7%20%E7%AC%AC3%E6%9C%9F |
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