Exploration of the antitumor activity and related mechanisms of RAD5 on colon cancer HT29 Cells
[Objectives] To investigate the antitumor activity and potential mechanisms of rosmarinic acid derivative 5 (RAD5) on colon cancer HT29 cells. [Methods] Colon cancer HT29 cells were treated with different concentrations of RAD5 (0 μmol/L, 12.5 μmol/L, 25 μmol/L, 50 μmol/L, 100 μmol/L, 150 μmol/L) fo...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Colorectal & Anal Surgery
2024-06-01
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| Series: | 结直肠肛门外科 |
| Subjects: | |
| Online Access: | https://jcas.gxmuyfy.cn/cn/wqll/paper.html?id=276&cateName=2024%E5%B9%B4%20%E7%AC%AC30%E5%8D%B7%20%E7%AC%AC3%E6%9C%9F |
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| Summary: | [Objectives] To investigate the antitumor activity and potential mechanisms of rosmarinic acid derivative 5 (RAD5) on colon cancer HT29 cells. [Methods] Colon cancer HT29 cells were treated with different concentrations of RAD5 (0 μmol/L, 12.5 μmol/L, 25 μmol/L, 50 μmol/L, 100 μmol/L, 150 μmol/L) for 36 hours, and cell viability was detected using the MTT assay. Colon cancer HT29 cells were divided into 3 groups and treated with RAD5 for 36 hours with 0 μmol/L, 25 μmol/L and 50 μmol/L respectively, the cell proliferation ability was assessed using a colony formation assay, apoptosis rate was detected by Hoechst 33258 staining and flow cytometry, and the expression of apoptosis-related proteins Bax, Bcl-2, and EGFR/MAPK pathway-related proteins was examined by Western blot. [Results] The results of cell experiments showed that RAD5 could significantly inhibit the proliferation of colon cancer HT29 cells in a concentration-dependent manner. Compared with the control group (0 μmol/L), the clone formation rates of the 25 μmol/L and 50 μmol/L groups were significantly reduced (P<0.001), apoptosis rates increased (P<0.01), Bax expression increased, Bcl-2 expression showed a downward trend, and the expression of EGFR and ERK1/2 proteins in the EGFR/MAPK pathway decreased, while the ratios of p-ERK1/2/ERK1/2 and p-P38/P38 were elevated (P<0.001). [Conclusion] RAD5 exhibits antitumor activity against colon cancer HT29 cell, and its mechanism may be related to inhibiting EGFR expression and activating the phosphorylation of ERK1/2 and P38 in the downstream MAPK pathway, providing experimental theoretical support for its development as an anticancer candidate drug. |
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| ISSN: | 1674-0491 |