Sputum Microbiota Compositions Correlate With Metabolome and Clinical Outcomes of COPD‐Bronchiectasis Association: A Prospective Cohort Study
ABSTRACT Bronchiectasis frequently co‐exists with chronic obstructive pulmonary disease (COPD‐bronchiectasis association [CBA]). We compared the microbiota and metabolome of bronchiectasis with (BO) and without airflow obstruction (BNO), COPD, and CBA. We determined how microbiota compositions corre...
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2025-08-01
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| Series: | Exploration |
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| Online Access: | https://doi.org/10.1002/EXP.20240149 |
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| author | Zhen‐feng He Xiao‐xian Zhang Cui‐xia Pan Xin‐zhu Yi Yan Huang Chun‐lan Chen Shan‐shan Zha Lai‐jian Cen Han‐qin Cai Lei Yang Jia‐qi Gao Hui‐min Li Zhen‐hong Lin Sheng‐zhu Lin Zhang Wang Nan‐shan Zhong Wei‐jie Guan |
| author_facet | Zhen‐feng He Xiao‐xian Zhang Cui‐xia Pan Xin‐zhu Yi Yan Huang Chun‐lan Chen Shan‐shan Zha Lai‐jian Cen Han‐qin Cai Lei Yang Jia‐qi Gao Hui‐min Li Zhen‐hong Lin Sheng‐zhu Lin Zhang Wang Nan‐shan Zhong Wei‐jie Guan |
| author_sort | Zhen‐feng He |
| collection | DOAJ |
| description | ABSTRACT Bronchiectasis frequently co‐exists with chronic obstructive pulmonary disease (COPD‐bronchiectasis association [CBA]). We compared the microbiota and metabolome of bronchiectasis with (BO) and without airflow obstruction (BNO), COPD, and CBA. We determined how microbiota compositions correlated with clinical characteristics and exacerbations of CBA. We prospectively recruited outpatients with BNO (n = 104), BO (n = 51), COPD (n = 33), and CBA (n = 70). We sampled at stead‐state and exacerbation, and profiled sputum microbiota via 16S rRNA sequencing and metabolome via liquid chromatography/mass spectrometry. Sputum microbiota and metabolome profiles of CBA separated from COPD (P < 0.05) but not bronchiectasis, partly driven by Proteobacteria enrichment in CBA. An increasing microbial interaction but not microbiota compositions were identified at exacerbation. Pseudomonadaceae‐dominant CBA yielded lower Shannon–Wiener diversity index (P < 0.001), greater bronchiectasis severity (P < 0.05) and higher future exacerbation risk (HR 2.46, 95% CI: 1.34–4.52, P < 0.001) than other genera‐dominant CBA. We found a clear metabolite discrimination between CBA and COPD. Most of up‐regulated metabolites identified in CBA, were amino acids metabolites, which indicated that the accumulation of amino acids metabolites was related to the alteration of airway microbiota. To conclude, airway structural changes, but not airflow limitation, correlate more profoundly with microbiota and metabolome profiles (e.g. partly via Pseudomonadaceae‐amino acids metabolism links), shaping clinical outcomes of CBA. |
| format | Article |
| id | doaj-art-59776605814c4b558bf825c5e9db6e4e |
| institution | Kabale University |
| issn | 2766-8509 2766-2098 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Exploration |
| spelling | doaj-art-59776605814c4b558bf825c5e9db6e4e2025-08-26T10:32:59ZengWileyExploration2766-85092766-20982025-08-0154n/an/a10.1002/EXP.20240149Sputum Microbiota Compositions Correlate With Metabolome and Clinical Outcomes of COPD‐Bronchiectasis Association: A Prospective Cohort StudyZhen‐feng He0Xiao‐xian Zhang1Cui‐xia Pan2Xin‐zhu Yi3Yan Huang4Chun‐lan Chen5Shan‐shan Zha6Lai‐jian Cen7Han‐qin Cai8Lei Yang9Jia‐qi Gao10Hui‐min Li11Zhen‐hong Lin12Sheng‐zhu Lin13Zhang Wang14Nan‐shan Zhong15Wei‐jie Guan16Department of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaDepartment of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaDepartment of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaInstitute of Ecological Sciences School of Life Sciences South China Normal University Guangzhou ChinaDepartment of Geriatrics National Key Clinical Specialty Guangzhou First People's Hospital South China University of Technology Guangzhou ChinaDepartment of Respiratory and Critical Care Medicine Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences Guangzhou ChinaDepartment of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaDepartment of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaInstitute of Ecological Sciences School of Life Sciences South China Normal University Guangzhou ChinaInstitute of Ecological Sciences School of Life Sciences South China Normal University Guangzhou ChinaInstitute of Ecological Sciences School of Life Sciences South China Normal University Guangzhou ChinaDepartment of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaDepartment of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaDepartment of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaInstitute of Ecological Sciences School of Life Sciences South China Normal University Guangzhou ChinaDepartment of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaDepartment of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University Guangzhou Guangdong P.R. ChinaABSTRACT Bronchiectasis frequently co‐exists with chronic obstructive pulmonary disease (COPD‐bronchiectasis association [CBA]). We compared the microbiota and metabolome of bronchiectasis with (BO) and without airflow obstruction (BNO), COPD, and CBA. We determined how microbiota compositions correlated with clinical characteristics and exacerbations of CBA. We prospectively recruited outpatients with BNO (n = 104), BO (n = 51), COPD (n = 33), and CBA (n = 70). We sampled at stead‐state and exacerbation, and profiled sputum microbiota via 16S rRNA sequencing and metabolome via liquid chromatography/mass spectrometry. Sputum microbiota and metabolome profiles of CBA separated from COPD (P < 0.05) but not bronchiectasis, partly driven by Proteobacteria enrichment in CBA. An increasing microbial interaction but not microbiota compositions were identified at exacerbation. Pseudomonadaceae‐dominant CBA yielded lower Shannon–Wiener diversity index (P < 0.001), greater bronchiectasis severity (P < 0.05) and higher future exacerbation risk (HR 2.46, 95% CI: 1.34–4.52, P < 0.001) than other genera‐dominant CBA. We found a clear metabolite discrimination between CBA and COPD. Most of up‐regulated metabolites identified in CBA, were amino acids metabolites, which indicated that the accumulation of amino acids metabolites was related to the alteration of airway microbiota. To conclude, airway structural changes, but not airflow limitation, correlate more profoundly with microbiota and metabolome profiles (e.g. partly via Pseudomonadaceae‐amino acids metabolism links), shaping clinical outcomes of CBA.https://doi.org/10.1002/EXP.20240149BronchiectasisCOPDCOPD‐Bronchiectasis associationexacerbationmetabolomemicrobiota |
| spellingShingle | Zhen‐feng He Xiao‐xian Zhang Cui‐xia Pan Xin‐zhu Yi Yan Huang Chun‐lan Chen Shan‐shan Zha Lai‐jian Cen Han‐qin Cai Lei Yang Jia‐qi Gao Hui‐min Li Zhen‐hong Lin Sheng‐zhu Lin Zhang Wang Nan‐shan Zhong Wei‐jie Guan Sputum Microbiota Compositions Correlate With Metabolome and Clinical Outcomes of COPD‐Bronchiectasis Association: A Prospective Cohort Study Exploration Bronchiectasis COPD COPD‐Bronchiectasis association exacerbation metabolome microbiota |
| title | Sputum Microbiota Compositions Correlate With Metabolome and Clinical Outcomes of COPD‐Bronchiectasis Association: A Prospective Cohort Study |
| title_full | Sputum Microbiota Compositions Correlate With Metabolome and Clinical Outcomes of COPD‐Bronchiectasis Association: A Prospective Cohort Study |
| title_fullStr | Sputum Microbiota Compositions Correlate With Metabolome and Clinical Outcomes of COPD‐Bronchiectasis Association: A Prospective Cohort Study |
| title_full_unstemmed | Sputum Microbiota Compositions Correlate With Metabolome and Clinical Outcomes of COPD‐Bronchiectasis Association: A Prospective Cohort Study |
| title_short | Sputum Microbiota Compositions Correlate With Metabolome and Clinical Outcomes of COPD‐Bronchiectasis Association: A Prospective Cohort Study |
| title_sort | sputum microbiota compositions correlate with metabolome and clinical outcomes of copd bronchiectasis association a prospective cohort study |
| topic | Bronchiectasis COPD COPD‐Bronchiectasis association exacerbation metabolome microbiota |
| url | https://doi.org/10.1002/EXP.20240149 |
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