Method for Linking a Synthesized Protein to Its mRNA-DNA Complex
A nascent protein remains in a complex with its ribosome and mRNA if the stop codon is deleted from the mRNA. In the same manner, mRNA forms a stable complex with DNA if the transcription termination is blocked. In principle, if both mRNA translation and DNA transcription termination are prevented,...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2000-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00294st06 |
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| _version_ | 1850151975582171136 |
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| author | Shumo Liu Ganaganor V. Shivashankar Tohru Sano Albert Libchaber |
| author_facet | Shumo Liu Ganaganor V. Shivashankar Tohru Sano Albert Libchaber |
| author_sort | Shumo Liu |
| collection | DOAJ |
| description | A nascent protein remains in a complex with its ribosome and mRNA if the stop codon is deleted from the mRNA. In the same manner, mRNA forms a stable complex with DNA if the transcription termination is blocked. In principle, if both mRNA translation and DNA transcription termination are prevented, the protein should stay in a complex with its mRNA and DNA. A method is designed to test these possibilities. Using an immobilized luciferase gene sequence, a functional luciferase protein is produced that remains associated through its mRNA with its DNA, confirming the feasibility of the proposed scheme. It has potential application for in vitro synthesis of proteins and protein micro-arrays. |
| format | Article |
| id | doaj-art-597573ef6fbd47cb98def0e5401526e6 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2000-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-597573ef6fbd47cb98def0e5401526e62025-08-20T02:26:06ZengTaylor & Francis GroupBioTechniques0736-62051940-98182000-10-0129479279810.2144/00294st06Method for Linking a Synthesized Protein to Its mRNA-DNA ComplexShumo Liu0Ganaganor V. Shivashankar1Tohru Sano2Albert Libchaber31NEC Research Institute, Princeton, NJ, USA1NEC Research Institute, Princeton, NJ, USA1NEC Research Institute, Princeton, NJ, USA1NEC Research Institute, Princeton, NJ, USAA nascent protein remains in a complex with its ribosome and mRNA if the stop codon is deleted from the mRNA. In the same manner, mRNA forms a stable complex with DNA if the transcription termination is blocked. In principle, if both mRNA translation and DNA transcription termination are prevented, the protein should stay in a complex with its mRNA and DNA. A method is designed to test these possibilities. Using an immobilized luciferase gene sequence, a functional luciferase protein is produced that remains associated through its mRNA with its DNA, confirming the feasibility of the proposed scheme. It has potential application for in vitro synthesis of proteins and protein micro-arrays.https://www.future-science.com/doi/10.2144/00294st06 |
| spellingShingle | Shumo Liu Ganaganor V. Shivashankar Tohru Sano Albert Libchaber Method for Linking a Synthesized Protein to Its mRNA-DNA Complex BioTechniques |
| title | Method for Linking a Synthesized Protein to Its mRNA-DNA Complex |
| title_full | Method for Linking a Synthesized Protein to Its mRNA-DNA Complex |
| title_fullStr | Method for Linking a Synthesized Protein to Its mRNA-DNA Complex |
| title_full_unstemmed | Method for Linking a Synthesized Protein to Its mRNA-DNA Complex |
| title_short | Method for Linking a Synthesized Protein to Its mRNA-DNA Complex |
| title_sort | method for linking a synthesized protein to its mrna dna complex |
| url | https://www.future-science.com/doi/10.2144/00294st06 |
| work_keys_str_mv | AT shumoliu methodforlinkingasynthesizedproteintoitsmrnadnacomplex AT ganaganorvshivashankar methodforlinkingasynthesizedproteintoitsmrnadnacomplex AT tohrusano methodforlinkingasynthesizedproteintoitsmrnadnacomplex AT albertlibchaber methodforlinkingasynthesizedproteintoitsmrnadnacomplex |