Isolation and Characterization of Paracoccus sp. GSM2 Capable of Degrading Textile Azo Dye Reactive Violet 5

A potential bacterial strain GSM2, capable of degrading an azo dye Reactive Violet 5 as a sole source of carbon, was isolated from textile mill effluent from Solapur, India. The 16S rDNA sequence and phenotypic characteristics indicated an isolated organism as Paracoccus sp. GSM2. This strain exhibi...

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Main Authors: Mallikarjun C. Bheemaraddi, Santosh Patil, Channappa T. Shivannavar, Subhashchandra M. Gaddad
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2014/410704
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author Mallikarjun C. Bheemaraddi
Santosh Patil
Channappa T. Shivannavar
Subhashchandra M. Gaddad
author_facet Mallikarjun C. Bheemaraddi
Santosh Patil
Channappa T. Shivannavar
Subhashchandra M. Gaddad
author_sort Mallikarjun C. Bheemaraddi
collection DOAJ
description A potential bacterial strain GSM2, capable of degrading an azo dye Reactive Violet 5 as a sole source of carbon, was isolated from textile mill effluent from Solapur, India. The 16S rDNA sequence and phenotypic characteristics indicated an isolated organism as Paracoccus sp. GSM2. This strain exhibited complete decolorization of Reactive Violet 5 (100 mg/L) within 16 h, while maximally it could decolorize 800 mg/L of dye within 38 h with 73% decolorization under static condition. For color removal, the most suitable pH and temperature were pH 6.0–9.0 and 25–40°C, respectively. The isolate was able to decolorize more than 70% of five structurally different azo dyes within 38 h. The isolate is salt tolerant as it can bring out more than 90% decolorization up to a salt concentration of 2% (w/v). UV-Visible absorption spectra before and after decolorization suggested that decolorization was due to biodegradation and was further confirmed by FT-IR spectroscopy. Overall results indicate the effectiveness of the strain GSM2 explored for the treatment of textile industry effluents containing various azo dyes. To our knowledge, this could be the first report on biodegradation of Reactive Violet 5 by Paracoccus sp. GSM2.
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institution Kabale University
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publishDate 2014-01-01
publisher Wiley
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series The Scientific World Journal
spelling doaj-art-5961a6549f884ce4b3ea016d574843642025-02-03T01:33:22ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/410704410704Isolation and Characterization of Paracoccus sp. GSM2 Capable of Degrading Textile Azo Dye Reactive Violet 5Mallikarjun C. Bheemaraddi0Santosh Patil1Channappa T. Shivannavar2Subhashchandra M. Gaddad3Department of Post Graduate Studies and Research in Microbiology, Gulbarga University, Gulbarga, Karnataka 585106, IndiaDepartment of Post Graduate Studies and Research in Microbiology, Gulbarga University, Gulbarga, Karnataka 585106, IndiaDepartment of Post Graduate Studies and Research in Microbiology, Gulbarga University, Gulbarga, Karnataka 585106, IndiaDepartment of Post Graduate Studies and Research in Microbiology, Gulbarga University, Gulbarga, Karnataka 585106, IndiaA potential bacterial strain GSM2, capable of degrading an azo dye Reactive Violet 5 as a sole source of carbon, was isolated from textile mill effluent from Solapur, India. The 16S rDNA sequence and phenotypic characteristics indicated an isolated organism as Paracoccus sp. GSM2. This strain exhibited complete decolorization of Reactive Violet 5 (100 mg/L) within 16 h, while maximally it could decolorize 800 mg/L of dye within 38 h with 73% decolorization under static condition. For color removal, the most suitable pH and temperature were pH 6.0–9.0 and 25–40°C, respectively. The isolate was able to decolorize more than 70% of five structurally different azo dyes within 38 h. The isolate is salt tolerant as it can bring out more than 90% decolorization up to a salt concentration of 2% (w/v). UV-Visible absorption spectra before and after decolorization suggested that decolorization was due to biodegradation and was further confirmed by FT-IR spectroscopy. Overall results indicate the effectiveness of the strain GSM2 explored for the treatment of textile industry effluents containing various azo dyes. To our knowledge, this could be the first report on biodegradation of Reactive Violet 5 by Paracoccus sp. GSM2.http://dx.doi.org/10.1155/2014/410704
spellingShingle Mallikarjun C. Bheemaraddi
Santosh Patil
Channappa T. Shivannavar
Subhashchandra M. Gaddad
Isolation and Characterization of Paracoccus sp. GSM2 Capable of Degrading Textile Azo Dye Reactive Violet 5
The Scientific World Journal
title Isolation and Characterization of Paracoccus sp. GSM2 Capable of Degrading Textile Azo Dye Reactive Violet 5
title_full Isolation and Characterization of Paracoccus sp. GSM2 Capable of Degrading Textile Azo Dye Reactive Violet 5
title_fullStr Isolation and Characterization of Paracoccus sp. GSM2 Capable of Degrading Textile Azo Dye Reactive Violet 5
title_full_unstemmed Isolation and Characterization of Paracoccus sp. GSM2 Capable of Degrading Textile Azo Dye Reactive Violet 5
title_short Isolation and Characterization of Paracoccus sp. GSM2 Capable of Degrading Textile Azo Dye Reactive Violet 5
title_sort isolation and characterization of paracoccus sp gsm2 capable of degrading textile azo dye reactive violet 5
url http://dx.doi.org/10.1155/2014/410704
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