Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle
Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four cop...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/3497401 |
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| author | I. Soria V. Quattrocchi C. Langellotti M. Pérez-Filgueira J. Pega V. Gnazzo S. Romera J. Schammas D. Bucafusco S. Di Giacomo Beatriz G. de la Torre D. Andreu F. Sobrino E. Blanco P. Zamorano |
| author_facet | I. Soria V. Quattrocchi C. Langellotti M. Pérez-Filgueira J. Pega V. Gnazzo S. Romera J. Schammas D. Bucafusco S. Di Giacomo Beatriz G. de la Torre D. Andreu F. Sobrino E. Blanco P. Zamorano |
| author_sort | I. Soria |
| collection | DOAJ |
| description | Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four copies of a peptide corresponding to the B-cell epitope VP1 [136–154] of type O FMDV (O/UKG/11/2001) linked through thioether bonds to a single copy of the T-cell epitope 3A [21–35] (termed B2T and B4T, resp.) afforded protection in vaccinated pigs. In this work, we show that dendrimeric peptides B2T and B4T can elicit specific humoral responses in cattle and confer partial protection against the challenge with a heterologous type O virus (O1/Campos/Bra/58). This protective response correlated with the induction of specific T-cells as well as with an anamnestic antibody response upon virus challenge, as shown by the detection of virus-specific antibody-secreting cells (ASC) in lymphoid tissues distal from the inoculation point. |
| format | Article |
| id | doaj-art-595e10e88c5e4be9b98f10dc80d56b31 |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-595e10e88c5e4be9b98f10dc80d56b312025-08-20T02:05:33ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/34974013497401Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in CattleI. Soria0V. Quattrocchi1C. Langellotti2M. Pérez-Filgueira3J. Pega4V. Gnazzo5S. Romera6J. Schammas7D. Bucafusco8S. Di Giacomo9Beatriz G. de la Torre10D. Andreu11F. Sobrino12E. Blanco13P. Zamorano14Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaInstituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaInstituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaInstituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaInstituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaInstituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaInstituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaInstituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaInstituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaDepartament de Ciencies Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, SpainDepartament de Ciencies Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, SpainCentro de Biología Molecular Severo Ochoa (CSIC-UAM), 28049 Madrid, SpainCentro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, SpainInstituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Buenos Aires, ArgentinaSynthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four copies of a peptide corresponding to the B-cell epitope VP1 [136–154] of type O FMDV (O/UKG/11/2001) linked through thioether bonds to a single copy of the T-cell epitope 3A [21–35] (termed B2T and B4T, resp.) afforded protection in vaccinated pigs. In this work, we show that dendrimeric peptides B2T and B4T can elicit specific humoral responses in cattle and confer partial protection against the challenge with a heterologous type O virus (O1/Campos/Bra/58). This protective response correlated with the induction of specific T-cells as well as with an anamnestic antibody response upon virus challenge, as shown by the detection of virus-specific antibody-secreting cells (ASC) in lymphoid tissues distal from the inoculation point.http://dx.doi.org/10.1155/2018/3497401 |
| spellingShingle | I. Soria V. Quattrocchi C. Langellotti M. Pérez-Filgueira J. Pega V. Gnazzo S. Romera J. Schammas D. Bucafusco S. Di Giacomo Beatriz G. de la Torre D. Andreu F. Sobrino E. Blanco P. Zamorano Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle Journal of Immunology Research |
| title | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_full | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_fullStr | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_full_unstemmed | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_short | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_sort | immune response and partial protection against heterologous foot and mouth disease virus induced by dendrimer peptides in cattle |
| url | http://dx.doi.org/10.1155/2018/3497401 |
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