Relevance of plant growth-promoting bacteria in reducing the severity of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici by altering metabolites and related genes
Among the biotic stresses, wilt disease severely affects tomato quality and productivity globally. The causal organism of this disease is Fusarium oxysporum f. sp. lycopersici (Fol), which is very well known and has a significant impact on the productivity of other crops as well. Efforts have been m...
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Frontiers Media S.A.
2025-01-01
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| author | Waquar Akhter Ansari Ram Krishna Sarvesh Pratap Kashyap Khalid Mashay Al-Anazi Mohammad Abul Farah Durgesh Kumar Jaiswal Akhilesh Yadav Mohammad Tarique Zeyad Jay Prakash Verma |
| author_facet | Waquar Akhter Ansari Ram Krishna Sarvesh Pratap Kashyap Khalid Mashay Al-Anazi Mohammad Abul Farah Durgesh Kumar Jaiswal Akhilesh Yadav Mohammad Tarique Zeyad Jay Prakash Verma |
| author_sort | Waquar Akhter Ansari |
| collection | DOAJ |
| description | Among the biotic stresses, wilt disease severely affects tomato quality and productivity globally. The causal organism of this disease is Fusarium oxysporum f. sp. lycopersici (Fol), which is very well known and has a significant impact on the productivity of other crops as well. Efforts have been made to investigate the effect of plant growth-promoting bacteria (PGPB) on alleviating tomato wilt disease. Four PGPB strains, such as Pseudomonas aeruginosa BHUPSB01 (T1), Pseudomonas putida BHUPSB04 (T2), Paenibacillus polymyxa BHUPSB16 (T3), and Bacillus cereus IESDJP-V4 (T4), were used as inocula to treat Fol-challenged plants. The results revealed that PGPB treatments T1, T2, T3, and T4 were able to decrease the severity of Fusarium wilt in the tomato plants at different levels. Among the treatments, T3 displayed the strongest protective effect, with the lowest disease frequency, which was 15.25%. There were no significant differences observed in parameters such as fruit yield and relative water content in the PGPB-inoculated plants, although T3 and T4 showed minimal electrolyte leakage. Significant changes in chlorophyll fluorescence were also recorded. A lower level of H2O2 and malondialdehyde (MDA) was observed in the T3 and T4 treatments. In addition, proline accumulation was highest in the T3-treated plants. Antioxidative enzyme activities, such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), significantly increased in the PGPB-treated plants. Furthermore, the highest phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) activity was reported in the T3 and T4 plants, respectively. The PGPB-treated plants showed elevated expression of the PAL, PPO, PR3, PR2, SOD, CAT, and PO genes. This study’s results reveal that PGPB strains can be utilized as biocontrol agents (BCAs) to enhance tomato resistance against Fusarium wilt. |
| format | Article |
| id | doaj-art-592ab7e0efbe43659cc6fe4557eb122a |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-592ab7e0efbe43659cc6fe4557eb122a2025-01-20T07:19:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.15347611534761Relevance of plant growth-promoting bacteria in reducing the severity of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici by altering metabolites and related genesWaquar Akhter Ansari0Ram Krishna1Sarvesh Pratap Kashyap2Khalid Mashay Al-Anazi3Mohammad Abul Farah4Durgesh Kumar Jaiswal5Akhilesh Yadav6Mohammad Tarique Zeyad7Jay Prakash Verma8Department of Agriculture, Faculty of Science, Marwadi University Research Center, Marwadi University, Rajkot, IndiaInstitute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, IndiaBio Zenith Scientific Research Pvt. Ltd., Mirzapur, IndiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Biotechnology, Graphic Era (Deemed to be University), Dehradun, IndiaDepartment of Plant Science, University of California, Davis, Davis, CA, United StatesDepartment of Agricultural Microbiology, Faculty of Agriculture Sciences, Aligarh Muslim University, Aligarh, IndiaInstitute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, IndiaAmong the biotic stresses, wilt disease severely affects tomato quality and productivity globally. The causal organism of this disease is Fusarium oxysporum f. sp. lycopersici (Fol), which is very well known and has a significant impact on the productivity of other crops as well. Efforts have been made to investigate the effect of plant growth-promoting bacteria (PGPB) on alleviating tomato wilt disease. Four PGPB strains, such as Pseudomonas aeruginosa BHUPSB01 (T1), Pseudomonas putida BHUPSB04 (T2), Paenibacillus polymyxa BHUPSB16 (T3), and Bacillus cereus IESDJP-V4 (T4), were used as inocula to treat Fol-challenged plants. The results revealed that PGPB treatments T1, T2, T3, and T4 were able to decrease the severity of Fusarium wilt in the tomato plants at different levels. Among the treatments, T3 displayed the strongest protective effect, with the lowest disease frequency, which was 15.25%. There were no significant differences observed in parameters such as fruit yield and relative water content in the PGPB-inoculated plants, although T3 and T4 showed minimal electrolyte leakage. Significant changes in chlorophyll fluorescence were also recorded. A lower level of H2O2 and malondialdehyde (MDA) was observed in the T3 and T4 treatments. In addition, proline accumulation was highest in the T3-treated plants. Antioxidative enzyme activities, such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), significantly increased in the PGPB-treated plants. Furthermore, the highest phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) activity was reported in the T3 and T4 plants, respectively. The PGPB-treated plants showed elevated expression of the PAL, PPO, PR3, PR2, SOD, CAT, and PO genes. This study’s results reveal that PGPB strains can be utilized as biocontrol agents (BCAs) to enhance tomato resistance against Fusarium wilt.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1534761/fullFusarium wilttomatoPGPBbio-primingantioxidative enzymegene expression analysis |
| spellingShingle | Waquar Akhter Ansari Ram Krishna Sarvesh Pratap Kashyap Khalid Mashay Al-Anazi Mohammad Abul Farah Durgesh Kumar Jaiswal Akhilesh Yadav Mohammad Tarique Zeyad Jay Prakash Verma Relevance of plant growth-promoting bacteria in reducing the severity of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici by altering metabolites and related genes Frontiers in Microbiology Fusarium wilt tomato PGPB bio-priming antioxidative enzyme gene expression analysis |
| title | Relevance of plant growth-promoting bacteria in reducing the severity of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici by altering metabolites and related genes |
| title_full | Relevance of plant growth-promoting bacteria in reducing the severity of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici by altering metabolites and related genes |
| title_fullStr | Relevance of plant growth-promoting bacteria in reducing the severity of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici by altering metabolites and related genes |
| title_full_unstemmed | Relevance of plant growth-promoting bacteria in reducing the severity of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici by altering metabolites and related genes |
| title_short | Relevance of plant growth-promoting bacteria in reducing the severity of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici by altering metabolites and related genes |
| title_sort | relevance of plant growth promoting bacteria in reducing the severity of tomato wilt caused by fusarium oxysporum f sp lycopersici by altering metabolites and related genes |
| topic | Fusarium wilt tomato PGPB bio-priming antioxidative enzyme gene expression analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1534761/full |
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