Long non-coding RNA CYTOR promotes the progression of melanoma via the miR-485-5p/GPI axis
Background Recent research has underscored the critical role of long non-coding RNAs (lncRNAs) in tumorigenesis and malignancy development. Nevertheless, the role of lncRNA cytoskeleton regulator RNA (CYTOR) in the progression of melanoma remains only partially elucidated. This research seeks to exp...
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PeerJ Inc.
2025-04-01
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| author | Haitao Lu Yunhua Zhao Yanli Zhang Shaomin Shi Huanrong Hu Xuefei Li Yandong Niu Haihua Qi Shang Ji Xinsuo Duan Yaling Liu |
| author_facet | Haitao Lu Yunhua Zhao Yanli Zhang Shaomin Shi Huanrong Hu Xuefei Li Yandong Niu Haihua Qi Shang Ji Xinsuo Duan Yaling Liu |
| author_sort | Haitao Lu |
| collection | DOAJ |
| description | Background Recent research has underscored the critical role of long non-coding RNAs (lncRNAs) in tumorigenesis and malignancy development. Nevertheless, the role of lncRNA cytoskeleton regulator RNA (CYTOR) in the progression of melanoma remains only partially elucidated. This research seeks to explore the impact of CYTOR on melanoma development and to elucidate the molecular mechanisms involved. Methods In vitro and in vivo models were used to assess CYTOR expression levels by QPCR and Western blotting. Melanoma cell proliferation, migration, and invasion were assessed by CCK-8 assay, scratch wound assay and transwell invasion experiments. The mechanism of CYTOR promoting melanoma progression was verified in a xenograft tumor mouse model. Results Our investigation identified a marked increase in CYTOR expression levels in both melanoma tissues and cells. Experiments conducted both in vitro and in vivo revealed that CYTOR markedly stimulated melanoma cell proliferation, migration, and invasion. Dual-luciferase reporter assays confirmed the direct binding of miR-485-5p to CYTOR, and glucose-6-phosphate isomerase (GPI) was identified as a direct target of miR-485-5p. |
| format | Article |
| id | doaj-art-59271d764d974dc69d06d4de7f066029 |
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| issn | 2167-8359 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj-art-59271d764d974dc69d06d4de7f0660292025-08-20T02:24:50ZengPeerJ Inc.PeerJ2167-83592025-04-0113e1928410.7717/peerj.19284Long non-coding RNA CYTOR promotes the progression of melanoma via the miR-485-5p/GPI axisHaitao Lu0Yunhua Zhao1Yanli Zhang2Shaomin Shi3Huanrong Hu4Xuefei Li5Yandong Niu6Haihua Qi7Shang Ji8Xinsuo Duan9Yaling Liu10Department of Dermatology, The Third Hospital of Hebei Medical University, Hebei, Shijiazhuang, ChinaDepartment of Otolaryngology, Chengde Central Hospital, Hebei, Chengde, ChinaDepartment of Dermatology, The Third Hospital of Hebei Medical University, Hebei, Shijiazhuang, ChinaDepartment of Dermatology, The Third Hospital of Hebei Medical University, Hebei, Shijiazhuang, ChinaDepartment of Dermatology, The Third Hospital of Hebei Medical University, Hebei, Shijiazhuang, ChinaDepartment of Dermatology, The Affiliated Hospital of Chengde Medical University, Hebei, Chengde, ChinaDepartment of Dermatology, The Affiliated Hospital of Chengde Medical University, Hebei, Chengde, ChinaDepartment of Dermatology, The Affiliated Hospital of Chengde Medical University, Hebei, Chengde, ChinaDepartment of Dermatology, The Third Hospital of Hebei Medical University, Hebei, Shijiazhuang, ChinaDepartment of Dermatology, The Affiliated Hospital of Chengde Medical University, Hebei, Chengde, ChinaDepartment of Dermatology, The Third Hospital of Hebei Medical University, Hebei, Shijiazhuang, ChinaBackground Recent research has underscored the critical role of long non-coding RNAs (lncRNAs) in tumorigenesis and malignancy development. Nevertheless, the role of lncRNA cytoskeleton regulator RNA (CYTOR) in the progression of melanoma remains only partially elucidated. This research seeks to explore the impact of CYTOR on melanoma development and to elucidate the molecular mechanisms involved. Methods In vitro and in vivo models were used to assess CYTOR expression levels by QPCR and Western blotting. Melanoma cell proliferation, migration, and invasion were assessed by CCK-8 assay, scratch wound assay and transwell invasion experiments. The mechanism of CYTOR promoting melanoma progression was verified in a xenograft tumor mouse model. Results Our investigation identified a marked increase in CYTOR expression levels in both melanoma tissues and cells. Experiments conducted both in vitro and in vivo revealed that CYTOR markedly stimulated melanoma cell proliferation, migration, and invasion. Dual-luciferase reporter assays confirmed the direct binding of miR-485-5p to CYTOR, and glucose-6-phosphate isomerase (GPI) was identified as a direct target of miR-485-5p.https://peerj.com/articles/19284.pdfMelanomaCYTORmiR-485-5pGPI |
| spellingShingle | Haitao Lu Yunhua Zhao Yanli Zhang Shaomin Shi Huanrong Hu Xuefei Li Yandong Niu Haihua Qi Shang Ji Xinsuo Duan Yaling Liu Long non-coding RNA CYTOR promotes the progression of melanoma via the miR-485-5p/GPI axis PeerJ Melanoma CYTOR miR-485-5p GPI |
| title | Long non-coding RNA CYTOR promotes the progression of melanoma via the miR-485-5p/GPI axis |
| title_full | Long non-coding RNA CYTOR promotes the progression of melanoma via the miR-485-5p/GPI axis |
| title_fullStr | Long non-coding RNA CYTOR promotes the progression of melanoma via the miR-485-5p/GPI axis |
| title_full_unstemmed | Long non-coding RNA CYTOR promotes the progression of melanoma via the miR-485-5p/GPI axis |
| title_short | Long non-coding RNA CYTOR promotes the progression of melanoma via the miR-485-5p/GPI axis |
| title_sort | long non coding rna cytor promotes the progression of melanoma via the mir 485 5p gpi axis |
| topic | Melanoma CYTOR miR-485-5p GPI |
| url | https://peerj.com/articles/19284.pdf |
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