Sample preparation in analytical peptidomics: It's all about timing
Identifying reliable biomarkers for rapid and accurate disease detection, prognosis, and treatment is becoming increasingly important. Peptides have recently emerged as a promising group in this domain. However, detailed information about their short-term analytical stability remains limited, from s...
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| Language: | English |
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Elsevier
2025-08-01
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| Series: | Talanta Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666831925000529 |
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| author | Amélie Descamps Bart De Spiegeleer Evelien Wynendaele Petar Naumovski Anton De Spiegeleer |
| author_facet | Amélie Descamps Bart De Spiegeleer Evelien Wynendaele Petar Naumovski Anton De Spiegeleer |
| author_sort | Amélie Descamps |
| collection | DOAJ |
| description | Identifying reliable biomarkers for rapid and accurate disease detection, prognosis, and treatment is becoming increasingly important. Peptides have recently emerged as a promising group in this domain. However, detailed information about their short-term analytical stability remains limited, from sample collection to the obtention of stabilized aliquots for UPLC-MS/MS analysis. This study assessed the impact of time-dependent sample preparation from blood and saliva on the stability of 17 structurally diverse quorum sensing peptides, a class of biologically active peptides. A significant time effect was observed, defined as the percentage ratio of slow versus rapid sample preparation under clinically relevant conditions. The average (± SEM) stability of the investigated peptides was found to be 55 (±19) % in blood and 32 (±22) % in saliva. A more detailed analysis of the kinetic profiles of five selected peptides revealed that metabolization (notably in blood) and adsorption (notably in saliva) were the primary factors influencing the observed time effect. The implications of these findings on detection limits were also discussed. This study highlights the critical importance of precise timings in analytical peptidomics procedures. |
| format | Article |
| id | doaj-art-5926e74671fa4178bfaf93d9524fa02a |
| institution | DOAJ |
| issn | 2666-8319 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Talanta Open |
| spelling | doaj-art-5926e74671fa4178bfaf93d9524fa02a2025-08-20T03:09:01ZengElsevierTalanta Open2666-83192025-08-011110045010.1016/j.talo.2025.100450Sample preparation in analytical peptidomics: It's all about timingAmélie Descamps0Bart De Spiegeleer1Evelien Wynendaele2Petar Naumovski3Anton De Spiegeleer4Drug Quality and Registration (DruQuaR) group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000, Ghent, Belgium; Translational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) Group, Ghent University Hospital, Corneel Heymanslaan 10, Ghent, BelgiumDrug Quality and Registration (DruQuaR) group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000, Ghent, Belgium; Translational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) Group, Ghent University Hospital, Corneel Heymanslaan 10, Ghent, BelgiumDrug Quality and Registration (DruQuaR) group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000, Ghent, Belgium; Translational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) Group, Ghent University Hospital, Corneel Heymanslaan 10, Ghent, BelgiumDrug Quality and Registration (DruQuaR) group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000, Ghent, Belgium; Translational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) Group, Ghent University Hospital, Corneel Heymanslaan 10, Ghent, BelgiumTranslational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) Group, Ghent University Hospital, Corneel Heymanslaan 10, Ghent, Belgium; Department of Geriatrics, Faculty of Medicine and Health Sciences, Ghent University Hospital, Corneel Heymanslaan 10, Ghent, Belgium; Corresponding author.Identifying reliable biomarkers for rapid and accurate disease detection, prognosis, and treatment is becoming increasingly important. Peptides have recently emerged as a promising group in this domain. However, detailed information about their short-term analytical stability remains limited, from sample collection to the obtention of stabilized aliquots for UPLC-MS/MS analysis. This study assessed the impact of time-dependent sample preparation from blood and saliva on the stability of 17 structurally diverse quorum sensing peptides, a class of biologically active peptides. A significant time effect was observed, defined as the percentage ratio of slow versus rapid sample preparation under clinically relevant conditions. The average (± SEM) stability of the investigated peptides was found to be 55 (±19) % in blood and 32 (±22) % in saliva. A more detailed analysis of the kinetic profiles of five selected peptides revealed that metabolization (notably in blood) and adsorption (notably in saliva) were the primary factors influencing the observed time effect. The implications of these findings on detection limits were also discussed. This study highlights the critical importance of precise timings in analytical peptidomics procedures.http://www.sciencedirect.com/science/article/pii/S2666831925000529Sample preparationAnalytical peptidomicsQuorum sensing peptidesTime effectsMetabolizationAdsorption |
| spellingShingle | Amélie Descamps Bart De Spiegeleer Evelien Wynendaele Petar Naumovski Anton De Spiegeleer Sample preparation in analytical peptidomics: It's all about timing Talanta Open Sample preparation Analytical peptidomics Quorum sensing peptides Time effects Metabolization Adsorption |
| title | Sample preparation in analytical peptidomics: It's all about timing |
| title_full | Sample preparation in analytical peptidomics: It's all about timing |
| title_fullStr | Sample preparation in analytical peptidomics: It's all about timing |
| title_full_unstemmed | Sample preparation in analytical peptidomics: It's all about timing |
| title_short | Sample preparation in analytical peptidomics: It's all about timing |
| title_sort | sample preparation in analytical peptidomics it s all about timing |
| topic | Sample preparation Analytical peptidomics Quorum sensing peptides Time effects Metabolization Adsorption |
| url | http://www.sciencedirect.com/science/article/pii/S2666831925000529 |
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