The Pathogenic Role of Expanded CD8⁺CD28<sup>null</sup> Angiogenic T Cells in ANCA-Associated Vasculitis
<b>Objectives:</b> Angiogenic T cells (Tang) are crucial in promoting angiogenesis, with the loss of CD28 serving as a marker for highly differentiated and senescent T cells. This study aims to investigate the characteristics and potential roles of CD8<sup>+</sup>CD28<sup&...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/13/1/26 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589044516651008 |
|---|---|
| author | Haomiao Shen Jinlin Miao Haoyang Sun Kui Zhang Renli Liu Zichao Li Leyang Zhang Peiyan Zhang Jiawei Wang Bei Zhang Longyu Chen Zhaohui Zheng Ping Zhu |
| author_facet | Haomiao Shen Jinlin Miao Haoyang Sun Kui Zhang Renli Liu Zichao Li Leyang Zhang Peiyan Zhang Jiawei Wang Bei Zhang Longyu Chen Zhaohui Zheng Ping Zhu |
| author_sort | Haomiao Shen |
| collection | DOAJ |
| description | <b>Objectives:</b> Angiogenic T cells (Tang) are crucial in promoting angiogenesis, with the loss of CD28 serving as a marker for highly differentiated and senescent T cells. This study aims to investigate the characteristics and potential roles of CD8<sup>+</sup>CD28<sup>null</sup> Tang in patients with ANCA-associated vasculitis (AAV). <b>Methods:</b> A cohort of AAV patients and matched healthy controls (HCs) were analyzed. Flow cytometry was used to assess the profiles of circulating CD8<sup>+</sup>CD28<sup>null</sup> Tang. In vitro functional assays were performed to evaluate the pathogenic properties of CD8<sup>+</sup>CD28<sup>null</sup> Tang. <b>Results</b>: CD8<sup>+</sup>CD28<sup>null</sup> Tang levels were significantly higher in the peripheral blood of AAV patients compared to HCs, and their levels were further increased in AAV patients with MPO⁺, p-ANCA⁺, or interstitial lung disease compared to their respective control groups. Additionally, there was a positive correlation between both the percentage and absolute count of CD8<sup>+</sup>CD28<sup>null</sup> Tang and the Birmingham Vasculitis Activity Score (BVAS). In patients with a good treatment response, both the percentage and absolute count of CD8<sup>+</sup>CD28<sup>null</sup> Tang were significantly reduced, and this reduction was positively correlated with the decrease in BVAS scores. In vitro studies revealed that CD8<sup>+</sup>CD28<sup>null</sup> Tang displayed enhanced chemotactic properties, induced apoptosis in human umbilical vein endothelial cells (HUVECs), and inhibited their proliferation, migration, and tube formation. <b>Conclusions:</b> AAV patients exhibit increased levels of circulating CD8<sup>+</sup>CD28<sup>null</sup> Tang, which can be reduced following effective treatment. Furthermore, CD8<sup>+</sup>CD28<sup>null</sup> Tang may contribute to the pathogenesis of AAV by promoting apoptosis and inhibiting the proliferation, migration, and tube formation of HUVECs. |
| format | Article |
| id | doaj-art-5926445728c64390aafb0a63df085870 |
| institution | Kabale University |
| issn | 2227-9059 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj-art-5926445728c64390aafb0a63df0858702025-01-24T13:23:46ZengMDPI AGBiomedicines2227-90592024-12-011312610.3390/biomedicines13010026The Pathogenic Role of Expanded CD8⁺CD28<sup>null</sup> Angiogenic T Cells in ANCA-Associated VasculitisHaomiao Shen0Jinlin Miao1Haoyang Sun2Kui Zhang3Renli Liu4Zichao Li5Leyang Zhang6Peiyan Zhang7Jiawei Wang8Bei Zhang9Longyu Chen10Zhaohui Zheng11Ping Zhu12Department of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Clinical Immunology of Xijing Hospital and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, China<b>Objectives:</b> Angiogenic T cells (Tang) are crucial in promoting angiogenesis, with the loss of CD28 serving as a marker for highly differentiated and senescent T cells. This study aims to investigate the characteristics and potential roles of CD8<sup>+</sup>CD28<sup>null</sup> Tang in patients with ANCA-associated vasculitis (AAV). <b>Methods:</b> A cohort of AAV patients and matched healthy controls (HCs) were analyzed. Flow cytometry was used to assess the profiles of circulating CD8<sup>+</sup>CD28<sup>null</sup> Tang. In vitro functional assays were performed to evaluate the pathogenic properties of CD8<sup>+</sup>CD28<sup>null</sup> Tang. <b>Results</b>: CD8<sup>+</sup>CD28<sup>null</sup> Tang levels were significantly higher in the peripheral blood of AAV patients compared to HCs, and their levels were further increased in AAV patients with MPO⁺, p-ANCA⁺, or interstitial lung disease compared to their respective control groups. Additionally, there was a positive correlation between both the percentage and absolute count of CD8<sup>+</sup>CD28<sup>null</sup> Tang and the Birmingham Vasculitis Activity Score (BVAS). In patients with a good treatment response, both the percentage and absolute count of CD8<sup>+</sup>CD28<sup>null</sup> Tang were significantly reduced, and this reduction was positively correlated with the decrease in BVAS scores. In vitro studies revealed that CD8<sup>+</sup>CD28<sup>null</sup> Tang displayed enhanced chemotactic properties, induced apoptosis in human umbilical vein endothelial cells (HUVECs), and inhibited their proliferation, migration, and tube formation. <b>Conclusions:</b> AAV patients exhibit increased levels of circulating CD8<sup>+</sup>CD28<sup>null</sup> Tang, which can be reduced following effective treatment. Furthermore, CD8<sup>+</sup>CD28<sup>null</sup> Tang may contribute to the pathogenesis of AAV by promoting apoptosis and inhibiting the proliferation, migration, and tube formation of HUVECs.https://www.mdpi.com/2227-9059/13/1/26angiogenic T cellsCD28<sup>null</sup>pathogenesisendothelial dysfunction |
| spellingShingle | Haomiao Shen Jinlin Miao Haoyang Sun Kui Zhang Renli Liu Zichao Li Leyang Zhang Peiyan Zhang Jiawei Wang Bei Zhang Longyu Chen Zhaohui Zheng Ping Zhu The Pathogenic Role of Expanded CD8⁺CD28<sup>null</sup> Angiogenic T Cells in ANCA-Associated Vasculitis Biomedicines angiogenic T cells CD28<sup>null</sup> pathogenesis endothelial dysfunction |
| title | The Pathogenic Role of Expanded CD8⁺CD28<sup>null</sup> Angiogenic T Cells in ANCA-Associated Vasculitis |
| title_full | The Pathogenic Role of Expanded CD8⁺CD28<sup>null</sup> Angiogenic T Cells in ANCA-Associated Vasculitis |
| title_fullStr | The Pathogenic Role of Expanded CD8⁺CD28<sup>null</sup> Angiogenic T Cells in ANCA-Associated Vasculitis |
| title_full_unstemmed | The Pathogenic Role of Expanded CD8⁺CD28<sup>null</sup> Angiogenic T Cells in ANCA-Associated Vasculitis |
| title_short | The Pathogenic Role of Expanded CD8⁺CD28<sup>null</sup> Angiogenic T Cells in ANCA-Associated Vasculitis |
| title_sort | pathogenic role of expanded cd8⁺cd28 sup null sup angiogenic t cells in anca associated vasculitis |
| topic | angiogenic T cells CD28<sup>null</sup> pathogenesis endothelial dysfunction |
| url | https://www.mdpi.com/2227-9059/13/1/26 |
| work_keys_str_mv | AT haomiaoshen thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT jinlinmiao thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT haoyangsun thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT kuizhang thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT renliliu thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT zichaoli thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT leyangzhang thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT peiyanzhang thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT jiaweiwang thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT beizhang thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT longyuchen thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT zhaohuizheng thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT pingzhu thepathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT haomiaoshen pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT jinlinmiao pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT haoyangsun pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT kuizhang pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT renliliu pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT zichaoli pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT leyangzhang pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT peiyanzhang pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT jiaweiwang pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT beizhang pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT longyuchen pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT zhaohuizheng pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis AT pingzhu pathogenicroleofexpandedcd8cd28supnullsupangiogenictcellsinancaassociatedvasculitis |