DNA-based cell typing in menstrual effluent identifies cell type variation by sample collection method: toward noninvasive biomarker development for women’s health
Menstrual effluent cell profiles have potential as noninvasive biomarkers of female reproductive and gynecological health and disease. We used DNA methylation-based cell type deconvolution (methylation cytometry) to identify cell type profiles in self-collected menstrual effluent. During the second...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Epigenetics |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15592294.2025.2453275 |
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| author | Irma M. Vlasac Hannah G. Stolrow Zaneta M. Thayer Brock C. Christensen Luisa Rivera |
| author_facet | Irma M. Vlasac Hannah G. Stolrow Zaneta M. Thayer Brock C. Christensen Luisa Rivera |
| author_sort | Irma M. Vlasac |
| collection | DOAJ |
| description | Menstrual effluent cell profiles have potential as noninvasive biomarkers of female reproductive and gynecological health and disease. We used DNA methylation-based cell type deconvolution (methylation cytometry) to identify cell type profiles in self-collected menstrual effluent. During the second day of their menstrual cycle, healthy participants collected menstrual effluent using a vaginal swab, menstrual cup, and pad. Immune cell proportions were highest in menstrual cup samples, and epithelial cells were highest in swab samples. Our work demonstrates the feasibility and utility of menstrual effluent cell profiling in population-level research using remotely collected samples and DNA methylation. |
| format | Article |
| id | doaj-art-591ebec91864423286ea94e96b8f88db |
| institution | Kabale University |
| issn | 1559-2294 1559-2308 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj-art-591ebec91864423286ea94e96b8f88db2025-01-18T15:28:59ZengTaylor & Francis GroupEpigenetics1559-22941559-23082025-12-0120110.1080/15592294.2025.2453275DNA-based cell typing in menstrual effluent identifies cell type variation by sample collection method: toward noninvasive biomarker development for women’s healthIrma M. Vlasac0Hannah G. Stolrow1Zaneta M. Thayer2Brock C. Christensen3Luisa Rivera4Department of Epidemiology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USADepartment of Epidemiology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USADepartment of Anthropology, Dartmouth College, Hanover, NH, USADepartment of Epidemiology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USADepartment of Anthropology, Dartmouth College, Hanover, NH, USAMenstrual effluent cell profiles have potential as noninvasive biomarkers of female reproductive and gynecological health and disease. We used DNA methylation-based cell type deconvolution (methylation cytometry) to identify cell type profiles in self-collected menstrual effluent. During the second day of their menstrual cycle, healthy participants collected menstrual effluent using a vaginal swab, menstrual cup, and pad. Immune cell proportions were highest in menstrual cup samples, and epithelial cells were highest in swab samples. Our work demonstrates the feasibility and utility of menstrual effluent cell profiling in population-level research using remotely collected samples and DNA methylation.https://www.tandfonline.com/doi/10.1080/15592294.2025.2453275Epigeneticswomens healthnoninvasivemenstrual effluentbiomarkersDNA methylation |
| spellingShingle | Irma M. Vlasac Hannah G. Stolrow Zaneta M. Thayer Brock C. Christensen Luisa Rivera DNA-based cell typing in menstrual effluent identifies cell type variation by sample collection method: toward noninvasive biomarker development for women’s health Epigenetics Epigenetics womens health noninvasive menstrual effluent biomarkers DNA methylation |
| title | DNA-based cell typing in menstrual effluent identifies cell type variation by sample collection method: toward noninvasive biomarker development for women’s health |
| title_full | DNA-based cell typing in menstrual effluent identifies cell type variation by sample collection method: toward noninvasive biomarker development for women’s health |
| title_fullStr | DNA-based cell typing in menstrual effluent identifies cell type variation by sample collection method: toward noninvasive biomarker development for women’s health |
| title_full_unstemmed | DNA-based cell typing in menstrual effluent identifies cell type variation by sample collection method: toward noninvasive biomarker development for women’s health |
| title_short | DNA-based cell typing in menstrual effluent identifies cell type variation by sample collection method: toward noninvasive biomarker development for women’s health |
| title_sort | dna based cell typing in menstrual effluent identifies cell type variation by sample collection method toward noninvasive biomarker development for women s health |
| topic | Epigenetics womens health noninvasive menstrual effluent biomarkers DNA methylation |
| url | https://www.tandfonline.com/doi/10.1080/15592294.2025.2453275 |
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