Exploring the polygenic landscape of wool traits in Turkish Merinos through multi-locus GWAS approaches: middle Anatolian Merino

Abstract This study investigates the genetic underpinnings of wool traits, specifically fibre diameter (FD) and staple length (SL), in Middle Anatolian Merino sheep using multi-locus genome-wide association study (GWAS) approaches. Representing the first attempt to examine these polygenic traits wit...

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Main Authors: Yalçın Yaman, A. Taner Önaldi, Şükrü Doğan, Mesut Kirbaş, Sedat Behrem, Yavuz Kal
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-95099-3
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author Yalçın Yaman
A. Taner Önaldi
Şükrü Doğan
Mesut Kirbaş
Sedat Behrem
Yavuz Kal
author_facet Yalçın Yaman
A. Taner Önaldi
Şükrü Doğan
Mesut Kirbaş
Sedat Behrem
Yavuz Kal
author_sort Yalçın Yaman
collection DOAJ
description Abstract This study investigates the genetic underpinnings of wool traits, specifically fibre diameter (FD) and staple length (SL), in Middle Anatolian Merino sheep using multi-locus genome-wide association study (GWAS) approaches. Representing the first attempt to examine these polygenic traits with multi-locus methods, the analysis employed four techniques: mrMLM, FASTmrMLM, FASTmrEMMA, and ISIS EM-BLASSO. A total of 18 Quantitative Trait Nucleotides (QTNs) were identified for FD, with 7 co-detected by multiple methods, and 14 QTNs were identified for SL, with 5 co-detected by multiple methods. Post-hoc power analysis revealed high statistical power for both traits (FD: 0.95, SL: 0.91). Notably, three candidate genes—PTPN3, TCF4, and ZBTB8A—were found to be consistent with prior studies. Gene enrichment and pathway analyses reaffirmed the complex and multifactorial molecular mechanisms governing wool traits. These findings enhance our understanding of the polygenic nature of wool traits, shedding light on the intricate genetic regulation and pinpointing genomic regions potentially influencing wool physiology. By identifying specific QTNs associated with FD and SL, this research provides a foundation for elucidating the genetic mechanisms underlying these economically significant traits. Upon validation in diverse populations, these findings hold substantial promise for the application of marker-assisted selection (MAS) to improve wool traits.
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spelling doaj-art-5904eaaa60d249d687683cddb2ddfefb2025-08-20T02:10:10ZengNature PortfolioScientific Reports2045-23222025-03-0115111410.1038/s41598-025-95099-3Exploring the polygenic landscape of wool traits in Turkish Merinos through multi-locus GWAS approaches: middle Anatolian MerinoYalçın Yaman0A. Taner Önaldi1Şükrü Doğan2Mesut Kirbaş3Sedat Behrem4Yavuz Kal5Department of Genetics, Faculty of Veterinary Medicine, Siirt UniversityBahri Dagtas International Agricultural Research InstituteBahri Dagtas International Agricultural Research InstituteBahri Dagtas International Agricultural Research InstituteDepartment of Genetics, Faculty of Veterinary Medicine, Aksaray UniversityBahri Dagtas International Agricultural Research InstituteAbstract This study investigates the genetic underpinnings of wool traits, specifically fibre diameter (FD) and staple length (SL), in Middle Anatolian Merino sheep using multi-locus genome-wide association study (GWAS) approaches. Representing the first attempt to examine these polygenic traits with multi-locus methods, the analysis employed four techniques: mrMLM, FASTmrMLM, FASTmrEMMA, and ISIS EM-BLASSO. A total of 18 Quantitative Trait Nucleotides (QTNs) were identified for FD, with 7 co-detected by multiple methods, and 14 QTNs were identified for SL, with 5 co-detected by multiple methods. Post-hoc power analysis revealed high statistical power for both traits (FD: 0.95, SL: 0.91). Notably, three candidate genes—PTPN3, TCF4, and ZBTB8A—were found to be consistent with prior studies. Gene enrichment and pathway analyses reaffirmed the complex and multifactorial molecular mechanisms governing wool traits. These findings enhance our understanding of the polygenic nature of wool traits, shedding light on the intricate genetic regulation and pinpointing genomic regions potentially influencing wool physiology. By identifying specific QTNs associated with FD and SL, this research provides a foundation for elucidating the genetic mechanisms underlying these economically significant traits. Upon validation in diverse populations, these findings hold substantial promise for the application of marker-assisted selection (MAS) to improve wool traits.https://doi.org/10.1038/s41598-025-95099-3Middle Anatolian MerinoWool traitsDual-purpose breedingMulti-locus GWASGrowth performance
spellingShingle Yalçın Yaman
A. Taner Önaldi
Şükrü Doğan
Mesut Kirbaş
Sedat Behrem
Yavuz Kal
Exploring the polygenic landscape of wool traits in Turkish Merinos through multi-locus GWAS approaches: middle Anatolian Merino
Scientific Reports
Middle Anatolian Merino
Wool traits
Dual-purpose breeding
Multi-locus GWAS
Growth performance
title Exploring the polygenic landscape of wool traits in Turkish Merinos through multi-locus GWAS approaches: middle Anatolian Merino
title_full Exploring the polygenic landscape of wool traits in Turkish Merinos through multi-locus GWAS approaches: middle Anatolian Merino
title_fullStr Exploring the polygenic landscape of wool traits in Turkish Merinos through multi-locus GWAS approaches: middle Anatolian Merino
title_full_unstemmed Exploring the polygenic landscape of wool traits in Turkish Merinos through multi-locus GWAS approaches: middle Anatolian Merino
title_short Exploring the polygenic landscape of wool traits in Turkish Merinos through multi-locus GWAS approaches: middle Anatolian Merino
title_sort exploring the polygenic landscape of wool traits in turkish merinos through multi locus gwas approaches middle anatolian merino
topic Middle Anatolian Merino
Wool traits
Dual-purpose breeding
Multi-locus GWAS
Growth performance
url https://doi.org/10.1038/s41598-025-95099-3
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