Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of Tpot with the Bromodeoxyuridine–DNA Flow Cytometry Technique?
Energy transfer in flow cytometry can occur when two fluorochromes are bound in close proximity (generally within 100 Å) and the emission spectrum of one fluorochrome overlaps significantly with the excitation spectrum of the other. The latter criterium is fullfilled for the fluorochromes fluorescei...
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| Format: | Article |
| Language: | English |
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Wiley
1999-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1999/364846 |
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| author | Maria C. Johansson Bo Baldetorp Stina M. Oredsson |
| author_facet | Maria C. Johansson Bo Baldetorp Stina M. Oredsson |
| author_sort | Maria C. Johansson |
| collection | DOAJ |
| description | Energy transfer in flow cytometry can occur when two fluorochromes are bound in close proximity (generally within 100 Å) and the emission spectrum of one fluorochrome overlaps significantly with the excitation spectrum of the other. The latter criterium is fullfilled for the fluorochromes fluorescein isothiocyanate and propidium iodide and also the former when they, e.g., are used in bromodeoxyuridine – DNA flow cytometry methods. In the present growth kinetic study using this method, we show that energy transfer does take place between fluorescein isothiocyanate and propidium iodide which results in a detected increase in DNA content with 2–3%. Despite the erroneous increase in the obtained DNA content values, this does not seem to have any influence on the calculation of DNA synthesis time and potential doubling time where the DNA content, based on the relative movement principle of the labelled cells, is used. |
| format | Article |
| id | doaj-art-590288dd3b6f422796f3af3faae2c74e |
| institution | OA Journals |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-590288dd3b6f422796f3af3faae2c74e2025-08-20T02:05:20ZengWileyAnalytical Cellular Pathology0921-89121878-36511999-01-01192919810.1155/1999/364846Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of Tpot with the Bromodeoxyuridine–DNA Flow Cytometry Technique?Maria C. Johansson0Bo Baldetorp1Stina M. Oredsson2The Jubileum Institute, Department of Oncology, University Hospital, S‐221 85 Lund, SwedenThe Jubileum Institute, Department of Oncology, University Hospital, S‐221 85 Lund, SwedenDepartment of Animal Physiology, University of Lund, S‐223 62 Lund, SwedenEnergy transfer in flow cytometry can occur when two fluorochromes are bound in close proximity (generally within 100 Å) and the emission spectrum of one fluorochrome overlaps significantly with the excitation spectrum of the other. The latter criterium is fullfilled for the fluorochromes fluorescein isothiocyanate and propidium iodide and also the former when they, e.g., are used in bromodeoxyuridine – DNA flow cytometry methods. In the present growth kinetic study using this method, we show that energy transfer does take place between fluorescein isothiocyanate and propidium iodide which results in a detected increase in DNA content with 2–3%. Despite the erroneous increase in the obtained DNA content values, this does not seem to have any influence on the calculation of DNA synthesis time and potential doubling time where the DNA content, based on the relative movement principle of the labelled cells, is used.http://dx.doi.org/10.1155/1999/364846 |
| spellingShingle | Maria C. Johansson Bo Baldetorp Stina M. Oredsson Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of Tpot with the Bromodeoxyuridine–DNA Flow Cytometry Technique? Analytical Cellular Pathology |
| title | Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of Tpot with the Bromodeoxyuridine–DNA Flow Cytometry Technique? |
| title_full | Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of Tpot with the Bromodeoxyuridine–DNA Flow Cytometry Technique? |
| title_fullStr | Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of Tpot with the Bromodeoxyuridine–DNA Flow Cytometry Technique? |
| title_full_unstemmed | Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of Tpot with the Bromodeoxyuridine–DNA Flow Cytometry Technique? |
| title_short | Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of Tpot with the Bromodeoxyuridine–DNA Flow Cytometry Technique? |
| title_sort | energy transfer between fluorescein isothiocyanate and propidium iodide a problem in the estimation of tpot with the bromodeoxyuridine dna flow cytometry technique |
| url | http://dx.doi.org/10.1155/1999/364846 |
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