Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women
Aim. To obtain a purified IgG preparation from the plasma of multiparous women and to evaluate its functional activity towards HLA-DR and HLA-G molecules.Materials and Methods. IgG preparation was prepared by means of affinity chromatography using the DEAE Affi-Gel Blue system. The purity of the iso...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Kemerovo State Medical University
2020-12-01
|
| Series: | Фундаментальная и клиническая медицина |
| Subjects: | |
| Online Access: | https://fcm.kemsmu.ru/jour/article/view/332 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849694851522625536 |
|---|---|
| author | A. V. Shabaldin N. S. Deeva A. S. Sukhikh G. V. Vavin |
| author_facet | A. V. Shabaldin N. S. Deeva A. S. Sukhikh G. V. Vavin |
| author_sort | A. V. Shabaldin |
| collection | DOAJ |
| description | Aim. To obtain a purified IgG preparation from the plasma of multiparous women and to evaluate its functional activity towards HLA-DR and HLA-G molecules.Materials and Methods. IgG preparation was prepared by means of affinity chromatography using the DEAE Affi-Gel Blue system. The purity of the isolated preparation was assessed by immunoelectrophoresis. Functional activity of the IgG preparation against HLA-DR and HLA-G molecules was assessed in peripheral blood lymphocytes isolated from 14 apparently healthy men of reproductive age by flow cytometry.Results. A 30 mL IgG fraction was obtained with a protein concentration of 4.3 g/l and a residual albumin less than 0.1 g/l. The protein concentration in the obtained fraction corresponded to the lower limit of IgG concentration in human serum. Immunoelectrophoresis showed that the IgG was the only antibody fraction in the chromatographic washout. Purified IgG fraction suppressed the expression of HLA-DR and HLA-G in donor lymphocytes.Conclusion. The purified IgG fraction from multiparous women can have a significant preventive and therapeutic effect against immune disorders in the mother-embryo system and therefore might halt the development of congenital heart defects by blocking HLA-DR and HLA-G in the lymphocytes. |
| format | Article |
| id | doaj-art-58fabe68735b4c90881c5205dd13f24a |
| institution | DOAJ |
| issn | 2500-0764 2542-0941 |
| language | Russian |
| publishDate | 2020-12-01 |
| publisher | Kemerovo State Medical University |
| record_format | Article |
| series | Фундаментальная и клиническая медицина |
| spelling | doaj-art-58fabe68735b4c90881c5205dd13f24a2025-08-20T03:19:57ZrusKemerovo State Medical UniversityФундаментальная и клиническая медицина2500-07642542-09412020-12-0154384510.23946/2500-0764-2020-5-4-38-45227Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous womenA. V. Shabaldin0N. S. Deeva1A. S. Sukhikh2G. V. Vavin3Research Institute for Complex Issues of Cardiovascular Diseases; Kemerovo State Medical UniversityResearch Institute for Complex Issues of Cardiovascular DiseasesKemerovo State Medical UniversityKemerovo State Medical UniversityAim. To obtain a purified IgG preparation from the plasma of multiparous women and to evaluate its functional activity towards HLA-DR and HLA-G molecules.Materials and Methods. IgG preparation was prepared by means of affinity chromatography using the DEAE Affi-Gel Blue system. The purity of the isolated preparation was assessed by immunoelectrophoresis. Functional activity of the IgG preparation against HLA-DR and HLA-G molecules was assessed in peripheral blood lymphocytes isolated from 14 apparently healthy men of reproductive age by flow cytometry.Results. A 30 mL IgG fraction was obtained with a protein concentration of 4.3 g/l and a residual albumin less than 0.1 g/l. The protein concentration in the obtained fraction corresponded to the lower limit of IgG concentration in human serum. Immunoelectrophoresis showed that the IgG was the only antibody fraction in the chromatographic washout. Purified IgG fraction suppressed the expression of HLA-DR and HLA-G in donor lymphocytes.Conclusion. The purified IgG fraction from multiparous women can have a significant preventive and therapeutic effect against immune disorders in the mother-embryo system and therefore might halt the development of congenital heart defects by blocking HLA-DR and HLA-G in the lymphocytes.https://fcm.kemsmu.ru/jour/article/view/332congenital heart diseaseigghla-drhla-gimmunoprevention |
| spellingShingle | A. V. Shabaldin N. S. Deeva A. S. Sukhikh G. V. Vavin Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women Фундаментальная и клиническая медицина congenital heart disease igg hla-dr hla-g immunoprevention |
| title | Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women |
| title_full | Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women |
| title_fullStr | Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women |
| title_full_unstemmed | Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women |
| title_short | Features of blocking HLA-DR and HLA-G BY the human IgG fraction from the plasma of multiparous women |
| title_sort | features of blocking hla dr and hla g by the human igg fraction from the plasma of multiparous women |
| topic | congenital heart disease igg hla-dr hla-g immunoprevention |
| url | https://fcm.kemsmu.ru/jour/article/view/332 |
| work_keys_str_mv | AT avshabaldin featuresofblockinghladrandhlagbythehumaniggfractionfromtheplasmaofmultiparouswomen AT nsdeeva featuresofblockinghladrandhlagbythehumaniggfractionfromtheplasmaofmultiparouswomen AT assukhikh featuresofblockinghladrandhlagbythehumaniggfractionfromtheplasmaofmultiparouswomen AT gvvavin featuresofblockinghladrandhlagbythehumaniggfractionfromtheplasmaofmultiparouswomen |