Comparison of air-liquid interface transwell and airway organoid models for human respiratory virus infection studies

Comparison of air-liquid interface transwell and airway organoid models for human respiratory virus infection studies

IntroductionComplex in vitro respiratory models, including air-liquid interface (ALI) transwell cultures and airway organoids, have emerged as promising tools for studying human respiratory virus infections. These models address several limitations of conventional two-dimensional cell line and anim...

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Main Authors: Camilla T. Ekanger, Nilima Dinesh Kumar, Rosanne W. Koutstaal, Fan Zhou, Martin Beukema, Joanna Waldock, Simon P. Jochems, Noa Mulder, Cécile A. C. M. van Els, Othmar G. Engelhardt, Nathalie Mantel, Kevin P. Buno, Karl Albert Brokstad, Agnete S. T. Engelsen, Rebecca J. Cox, Barbro N. Melgert, Anke L. W. Huckriede, Puck B. van Kasteren
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-02-01
Series:Frontiers in Immunology
Subjects:
influenza virus
mucosal models
respiratory tract
complex in vitro models
harmonization
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2025.1532144/full
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