Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene
ABSTRACT This study aimed to analyze the genetic diversity of bacteria associated in bioflocs using 16S-rRNA polymerase chain reaction (PCR) with ARDRA technique. A total of 38 dominant bacterial isolates was obtained from bioflocs samples and of these isolates, 16S-rRNA gene was then isolated and...
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Asosiasi Sains Akuakultur Indonesia
2015-05-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/9367 |
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| author | , Widanarni Dewi Nurhayati Dinamella Wahjuningrum |
| author_facet | , Widanarni Dewi Nurhayati Dinamella Wahjuningrum |
| author_sort | , Widanarni |
| collection | DOAJ |
| description | ABSTRACT
This study aimed to analyze the genetic diversity of bacteria associated in bioflocs using 16S-rRNA polymerase chain reaction (PCR) with ARDRA technique. A total of 38 dominant bacterial isolates was obtained from bioflocs samples and of these isolates, 16S-rRNA gene was then isolated and amplified using PCR. The 16S-rRNA gene of the isolates was then cut using HaeIII (5’-GG↓CC) and HhaI (5’-GCG↓C) restriction enzymes resulting an ARDRA pattern which was further used as the binary data for the construction of phylogenetics tree that was used to estimate the group of bacteria. The result with HaeIII cut restriction enzyme from biofloc-associated bacteria gave 11 ARDRA patterns, while with the restriction enzyme HhaI gave eight ARDRA patterns. Phylogenetics of bacterial populations from biofloc-based cultivation system water consisted of at least 13 different bacterial species. Result of sequencing from two gene sample 16S-rRNA were identified as Microbacterium foliorumand and Pseudomonas putida.
Keywords: bacterial diversity, ARDRA, biofloc, phylogeny
ABSTRAK
Penelitian ini bertujuan untuk menganalisis keragaman genetika bakteri bioflok menggunakan metode polymerase chain reaction (PCR) 16S-rRNA dengan teknik ARDRA. Sebanyak 38 isolat bakteri dominan yang diperoleh diamplifikasi gen 16S-rRNAnya dengan PCR, kemudian dipotong dengan enzim restriksi HaeIII (5’-GG↓CC) dan HhaI (5’-GCG↓C). Pola ARDRA ini dijadikan data biner sebagai input untuk konstruksi pohon filogenetika yang dapat digunakan untuk memerkirakan jenis bakteri yang ada. Gen 16S-rRNA hasil PCR setelah dipotong dengan enzim restriksi HaeIII didapatkan 11 pola ARDRA, sedangkan dengan enzim restriksi HhaI menghasilkan delapan pola ARDRA. Berdasarkan pohon filogenetika, diketahui populasi bakteri pada air sistem budidaya bioflok sedikitnya terdiri atas 13 jenis bakteri. Berdasarkan sekuensing dari dua sampel gen 16S-rRNA teridentifikasi jenis bakteri Microbacterium foliorum dan Pseudomonas putida.
Kata kunci: keragaman bakteri, ARDRA, bioflok, filogenetika
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| format | Article |
| id | doaj-art-58e154ff75cf4e6dbc560bc554a62272 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2015-05-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-58e154ff75cf4e6dbc560bc554a622722025-01-24T17:59:20ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-05-0112210.19027/jai.12.128-1357831Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene, Widanarni0Dewi NurhayatiDinamella Wahjuningrum1Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680ABSTRACT This study aimed to analyze the genetic diversity of bacteria associated in bioflocs using 16S-rRNA polymerase chain reaction (PCR) with ARDRA technique. A total of 38 dominant bacterial isolates was obtained from bioflocs samples and of these isolates, 16S-rRNA gene was then isolated and amplified using PCR. The 16S-rRNA gene of the isolates was then cut using HaeIII (5’-GG↓CC) and HhaI (5’-GCG↓C) restriction enzymes resulting an ARDRA pattern which was further used as the binary data for the construction of phylogenetics tree that was used to estimate the group of bacteria. The result with HaeIII cut restriction enzyme from biofloc-associated bacteria gave 11 ARDRA patterns, while with the restriction enzyme HhaI gave eight ARDRA patterns. Phylogenetics of bacterial populations from biofloc-based cultivation system water consisted of at least 13 different bacterial species. Result of sequencing from two gene sample 16S-rRNA were identified as Microbacterium foliorumand and Pseudomonas putida. Keywords: bacterial diversity, ARDRA, biofloc, phylogeny ABSTRAK Penelitian ini bertujuan untuk menganalisis keragaman genetika bakteri bioflok menggunakan metode polymerase chain reaction (PCR) 16S-rRNA dengan teknik ARDRA. Sebanyak 38 isolat bakteri dominan yang diperoleh diamplifikasi gen 16S-rRNAnya dengan PCR, kemudian dipotong dengan enzim restriksi HaeIII (5’-GG↓CC) dan HhaI (5’-GCG↓C). Pola ARDRA ini dijadikan data biner sebagai input untuk konstruksi pohon filogenetika yang dapat digunakan untuk memerkirakan jenis bakteri yang ada. Gen 16S-rRNA hasil PCR setelah dipotong dengan enzim restriksi HaeIII didapatkan 11 pola ARDRA, sedangkan dengan enzim restriksi HhaI menghasilkan delapan pola ARDRA. Berdasarkan pohon filogenetika, diketahui populasi bakteri pada air sistem budidaya bioflok sedikitnya terdiri atas 13 jenis bakteri. Berdasarkan sekuensing dari dua sampel gen 16S-rRNA teridentifikasi jenis bakteri Microbacterium foliorum dan Pseudomonas putida. Kata kunci: keragaman bakteri, ARDRA, bioflok, filogenetika https://journal.ipb.ac.id/index.php/jai/article/view/9367 |
| spellingShingle | , Widanarni Dewi Nurhayati Dinamella Wahjuningrum Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene Jurnal Akuakultur Indonesia |
| title | Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene |
| title_full | Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene |
| title_fullStr | Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene |
| title_full_unstemmed | Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene |
| title_short | Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene |
| title_sort | analysis of bacterial genetic diversity in biofloc by using ardra 16s rrna gene |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/9367 |
| work_keys_str_mv | AT widanarni analysisofbacterialgeneticdiversityinbioflocbyusingardra16srrnagene AT dewinurhayati analysisofbacterialgeneticdiversityinbioflocbyusingardra16srrnagene AT dinamellawahjuningrum analysisofbacterialgeneticdiversityinbioflocbyusingardra16srrnagene |