Tamarixetin: A Promising Bioflavonoid Against Acetaminophen-Induced Liver Injury
Oxidative stress, mitochondrial dysfunction, and inflammatory responses cause acute liver failure in most cases of acetaminophen (APAP) overdose. Tamarixetin (Trx), an antioxidant and anti-inflammatory flavonoid, has not yet been studied in models of APAP-induced hepatotoxicity. Trx was tested for i...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/7/524 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849734036178599936 |
|---|---|
| author | Mehmet Ali Telafarlı Ejder Saylav Bora Firdes Topal Oytun Erbaş |
| author_facet | Mehmet Ali Telafarlı Ejder Saylav Bora Firdes Topal Oytun Erbaş |
| author_sort | Mehmet Ali Telafarlı |
| collection | DOAJ |
| description | Oxidative stress, mitochondrial dysfunction, and inflammatory responses cause acute liver failure in most cases of acetaminophen (APAP) overdose. Tamarixetin (Trx), an antioxidant and anti-inflammatory flavonoid, has not yet been studied in models of APAP-induced hepatotoxicity. Trx was tested for its protective effects on APAP-induced liver injury in rats using biochemical, histopathological, and oxidative stress parameters. Three groups of 30 male Wistar rats were randomly assigned to the following groups: control, APAP + Saline, and APAP + Trx (3 mg/kg/day, intraperitoneally for 3 days). A single 300 mg/kg intraperitoneal APAP dose caused hepatotoxicity. ALT, MDA, GSH, HSP-70, and thioredoxin were measured in blood and liver tissues. Liver sections were histopathologically examined. APAP depleted hepatic GSH and Trx and increased serum ALT and MDA. Trx treatment significantly reduced ALT (201.2 → 105.1 U/L), MDA (5.5 → 3.4 nmol/mg), and the percentage of histologically damaged hepatocytes (58.5% → 9.5%), while restoring GSH and thioredoxin levels. Notably, HSP-70 expression exceeded that of APAP and control levels, suggesting the modulation of the stress response. The Trx group showed significant hepatoprotection histologically. Trx reduces APAP-induced hepatic damage, likely through antioxidant and anti-inflammatory mechanisms. These findings suggest that Trx may be a natural hepatoprotectant, warranting clinical trials. |
| format | Article |
| id | doaj-art-58e002b8dceb48ea835f9fa3c9a6c922 |
| institution | DOAJ |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-58e002b8dceb48ea835f9fa3c9a6c9222025-08-20T03:07:54ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-07-0147752410.3390/cimb47070524Tamarixetin: A Promising Bioflavonoid Against Acetaminophen-Induced Liver InjuryMehmet Ali Telafarlı0Ejder Saylav Bora1Firdes Topal2Oytun Erbaş3Department of Emergency Medicine, Erzurum City Hospital, 25240 Erzurum, TurkeyDepartment of Emergency Medicine, Faculty of Medicine, Izmir Katip Çelebi University, 35360 Izmir, TurkeyDepartment of Gastroenterology, Faculty of Medicine, Izmir Katip Çelebi University, 35360 Izmir, TurkeyBiruni Research Center (BAMER), Faculty of Medicine, Biruni University, 34015 Istanbul, TurkeyOxidative stress, mitochondrial dysfunction, and inflammatory responses cause acute liver failure in most cases of acetaminophen (APAP) overdose. Tamarixetin (Trx), an antioxidant and anti-inflammatory flavonoid, has not yet been studied in models of APAP-induced hepatotoxicity. Trx was tested for its protective effects on APAP-induced liver injury in rats using biochemical, histopathological, and oxidative stress parameters. Three groups of 30 male Wistar rats were randomly assigned to the following groups: control, APAP + Saline, and APAP + Trx (3 mg/kg/day, intraperitoneally for 3 days). A single 300 mg/kg intraperitoneal APAP dose caused hepatotoxicity. ALT, MDA, GSH, HSP-70, and thioredoxin were measured in blood and liver tissues. Liver sections were histopathologically examined. APAP depleted hepatic GSH and Trx and increased serum ALT and MDA. Trx treatment significantly reduced ALT (201.2 → 105.1 U/L), MDA (5.5 → 3.4 nmol/mg), and the percentage of histologically damaged hepatocytes (58.5% → 9.5%), while restoring GSH and thioredoxin levels. Notably, HSP-70 expression exceeded that of APAP and control levels, suggesting the modulation of the stress response. The Trx group showed significant hepatoprotection histologically. Trx reduces APAP-induced hepatic damage, likely through antioxidant and anti-inflammatory mechanisms. These findings suggest that Trx may be a natural hepatoprotectant, warranting clinical trials.https://www.mdpi.com/1467-3045/47/7/524tamarixetinacetaminophenhepatotoxicityoxidative stressliver protection |
| spellingShingle | Mehmet Ali Telafarlı Ejder Saylav Bora Firdes Topal Oytun Erbaş Tamarixetin: A Promising Bioflavonoid Against Acetaminophen-Induced Liver Injury Current Issues in Molecular Biology tamarixetin acetaminophen hepatotoxicity oxidative stress liver protection |
| title | Tamarixetin: A Promising Bioflavonoid Against Acetaminophen-Induced Liver Injury |
| title_full | Tamarixetin: A Promising Bioflavonoid Against Acetaminophen-Induced Liver Injury |
| title_fullStr | Tamarixetin: A Promising Bioflavonoid Against Acetaminophen-Induced Liver Injury |
| title_full_unstemmed | Tamarixetin: A Promising Bioflavonoid Against Acetaminophen-Induced Liver Injury |
| title_short | Tamarixetin: A Promising Bioflavonoid Against Acetaminophen-Induced Liver Injury |
| title_sort | tamarixetin a promising bioflavonoid against acetaminophen induced liver injury |
| topic | tamarixetin acetaminophen hepatotoxicity oxidative stress liver protection |
| url | https://www.mdpi.com/1467-3045/47/7/524 |
| work_keys_str_mv | AT mehmetalitelafarlı tamarixetinapromisingbioflavonoidagainstacetaminopheninducedliverinjury AT ejdersaylavbora tamarixetinapromisingbioflavonoidagainstacetaminopheninducedliverinjury AT firdestopal tamarixetinapromisingbioflavonoidagainstacetaminopheninducedliverinjury AT oytunerbas tamarixetinapromisingbioflavonoidagainstacetaminopheninducedliverinjury |