High-Mobility Group Box-1 and Endothelial Cell Angiogenic Markers in the Vitreous from Patients with Proliferative Diabetic Retinopathy

The aim of this study was to measure the levels of high-mobility group box-1 (HMGB1) in the vitreous fluid from patients with proliferative diabetic retinopathy (PDR) and to correlate its levels with clinical disease activity and the levels of vascular endothelial growth factor (VEGF), the angiogeni...

Full description

Saved in:
Bibliographic Details
Main Authors: Ahmed M. Abu El-Asrar, Mohd Imtiaz Nawaz, Dustan Kangave, Marwan Abouammoh, Ghulam Mohammad
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2012/697489
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832554355524370432
author Ahmed M. Abu El-Asrar
Mohd Imtiaz Nawaz
Dustan Kangave
Marwan Abouammoh
Ghulam Mohammad
author_facet Ahmed M. Abu El-Asrar
Mohd Imtiaz Nawaz
Dustan Kangave
Marwan Abouammoh
Ghulam Mohammad
author_sort Ahmed M. Abu El-Asrar
collection DOAJ
description The aim of this study was to measure the levels of high-mobility group box-1 (HMGB1) in the vitreous fluid from patients with proliferative diabetic retinopathy (PDR) and to correlate its levels with clinical disease activity and the levels of vascular endothelial growth factor (VEGF), the angiogenic cytokine granulocyte-colony-stimulating factor (G-CSF), the endothelial cell angiogenic markers soluble vascular endothelial-cadherin (sVE-cadherin), and soluble endoglin (sEng). Vitreous samples from 36 PDR and 21 nondiabetic patients were studied by enzyme-linked immunosorbent assay. HMGB1, VEGF, sVE-cadherin, and sEng levels were significantly higher in PDR patients than in nondiabetics (P=0.008; <0.001; <0.001; 0.003, resp.). G-CSF was detected in only 3 PDR samples. In the whole study group, there was significant positive correlation between the levels of HMGB1, and sVE-cadherin (r=0.378, P=0.007). In PDR patients, there was significant negative correlation between the levels of sVE-cadherin and sEng (r=−0.517, P=0.0005). Exploratory regression analysis identified significant associations between active PDR and high levels of VEGF (odds ratio = 76.4; 95% confidence interval = 6.32–923) and high levels of sEng (odds ratio = 6.01; 95% confidence interval = 1.25–29.0). Our findings suggest that HMGB1, VEGF, sVE-cadherin and sEng regulate the angiogenesis in PDR.
format Article
id doaj-art-58dcd25b9fe947d9a04ef1e7821c643c
institution Kabale University
issn 0962-9351
1466-1861
language English
publishDate 2012-01-01
publisher Wiley
record_format Article
series Mediators of Inflammation
spelling doaj-art-58dcd25b9fe947d9a04ef1e7821c643c2025-02-03T05:51:44ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/697489697489High-Mobility Group Box-1 and Endothelial Cell Angiogenic Markers in the Vitreous from Patients with Proliferative Diabetic RetinopathyAhmed M. Abu El-Asrar0Mohd Imtiaz Nawaz1Dustan Kangave2Marwan Abouammoh3Ghulam Mohammad4Department of Ophthalmology, College of Medicine, King Saud University, Riyadh 11411, Saudi ArabiaDepartment of Ophthalmology, College of Medicine, King Saud University, Riyadh 11411, Saudi ArabiaDepartment of Ophthalmology, College of Medicine, King Saud University, Riyadh 11411, Saudi ArabiaDepartment of Ophthalmology, College of Medicine, King Saud University, Riyadh 11411, Saudi ArabiaDepartment of Ophthalmology, College of Medicine, King Saud University, Riyadh 11411, Saudi ArabiaThe aim of this study was to measure the levels of high-mobility group box-1 (HMGB1) in the vitreous fluid from patients with proliferative diabetic retinopathy (PDR) and to correlate its levels with clinical disease activity and the levels of vascular endothelial growth factor (VEGF), the angiogenic cytokine granulocyte-colony-stimulating factor (G-CSF), the endothelial cell angiogenic markers soluble vascular endothelial-cadherin (sVE-cadherin), and soluble endoglin (sEng). Vitreous samples from 36 PDR and 21 nondiabetic patients were studied by enzyme-linked immunosorbent assay. HMGB1, VEGF, sVE-cadherin, and sEng levels were significantly higher in PDR patients than in nondiabetics (P=0.008; <0.001; <0.001; 0.003, resp.). G-CSF was detected in only 3 PDR samples. In the whole study group, there was significant positive correlation between the levels of HMGB1, and sVE-cadherin (r=0.378, P=0.007). In PDR patients, there was significant negative correlation between the levels of sVE-cadherin and sEng (r=−0.517, P=0.0005). Exploratory regression analysis identified significant associations between active PDR and high levels of VEGF (odds ratio = 76.4; 95% confidence interval = 6.32–923) and high levels of sEng (odds ratio = 6.01; 95% confidence interval = 1.25–29.0). Our findings suggest that HMGB1, VEGF, sVE-cadherin and sEng regulate the angiogenesis in PDR.http://dx.doi.org/10.1155/2012/697489
spellingShingle Ahmed M. Abu El-Asrar
Mohd Imtiaz Nawaz
Dustan Kangave
Marwan Abouammoh
Ghulam Mohammad
High-Mobility Group Box-1 and Endothelial Cell Angiogenic Markers in the Vitreous from Patients with Proliferative Diabetic Retinopathy
Mediators of Inflammation
title High-Mobility Group Box-1 and Endothelial Cell Angiogenic Markers in the Vitreous from Patients with Proliferative Diabetic Retinopathy
title_full High-Mobility Group Box-1 and Endothelial Cell Angiogenic Markers in the Vitreous from Patients with Proliferative Diabetic Retinopathy
title_fullStr High-Mobility Group Box-1 and Endothelial Cell Angiogenic Markers in the Vitreous from Patients with Proliferative Diabetic Retinopathy
title_full_unstemmed High-Mobility Group Box-1 and Endothelial Cell Angiogenic Markers in the Vitreous from Patients with Proliferative Diabetic Retinopathy
title_short High-Mobility Group Box-1 and Endothelial Cell Angiogenic Markers in the Vitreous from Patients with Proliferative Diabetic Retinopathy
title_sort high mobility group box 1 and endothelial cell angiogenic markers in the vitreous from patients with proliferative diabetic retinopathy
url http://dx.doi.org/10.1155/2012/697489
work_keys_str_mv AT ahmedmabuelasrar highmobilitygroupbox1andendothelialcellangiogenicmarkersinthevitreousfrompatientswithproliferativediabeticretinopathy
AT mohdimtiaznawaz highmobilitygroupbox1andendothelialcellangiogenicmarkersinthevitreousfrompatientswithproliferativediabeticretinopathy
AT dustankangave highmobilitygroupbox1andendothelialcellangiogenicmarkersinthevitreousfrompatientswithproliferativediabeticretinopathy
AT marwanabouammoh highmobilitygroupbox1andendothelialcellangiogenicmarkersinthevitreousfrompatientswithproliferativediabeticretinopathy
AT ghulammohammad highmobilitygroupbox1andendothelialcellangiogenicmarkersinthevitreousfrompatientswithproliferativediabeticretinopathy