Simultaneous Encapsulation of Probiotic Bacteria (<i>Lactococcus lactis</i>, and <i>Lactiplantibacillus plantarum</i>) in Calcium Alginate Hydrogels
Encapsulation in alginate hydrogel microspheres is an effective method for protecting and improving the survival of lactic acid bacteria in different environments. This research aims to expand the knowledge about the structure/property relationship of calcium alginate microspheres loaded with a mixt...
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2025-01-01
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| author | Marko Vinceković Luna Maslov Bandić Fabijan Oštarić Marta Kiš Nevijo Zdolec Ivan Marić Suzana Šegota Hana Zelić Nataša Mikulec |
| author_facet | Marko Vinceković Luna Maslov Bandić Fabijan Oštarić Marta Kiš Nevijo Zdolec Ivan Marić Suzana Šegota Hana Zelić Nataša Mikulec |
| author_sort | Marko Vinceković |
| collection | DOAJ |
| description | Encapsulation in alginate hydrogel microspheres is an effective method for protecting and improving the survival of lactic acid bacteria in different environments. This research aims to expand the knowledge about the structure/property relationship of calcium alginate microspheres loaded with a mixture of autochthonous probiotic bacteria (<i>Lactococcus lactis</i> and <i>Lactiplantibacillus plantarum</i>). A novel hydrogel formulation (FORMLAB) was prepared by ionic gelation and the molecular interactions between the FORMLAB constituents, surface morphology, structure, swelling degree, and release profile were characterized. The simultaneous encapsulation of two bacterial cultures in the same compartment does not diminish their viability. The binding of calcium ions to bacterial cells creates favorable conditions for the propagation of the encapsulated bacteria. The molecular interactions between the FORMLAB constituents are complex, involving mainly hydrogen bonds and electrostatic interactions. With a very high degree of swelling followed by low crosslinking, the surface of the microspheres covered with bacterial cells and diffusion through the hydrogel matrix allow for the delivery of probiotics at the right time. The findings suggest that bacterial cells are efficiently delivered from calcium alginate microspheres, offering promising applications in the development of functional foods, especially in cheese production. |
| format | Article |
| id | doaj-art-58d671c1897140ebad219bccdc25e918 |
| institution | Kabale University |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Gels |
| spelling | doaj-art-58d671c1897140ebad219bccdc25e9182025-01-24T13:33:51ZengMDPI AGGels2310-28612025-01-011113410.3390/gels11010034Simultaneous Encapsulation of Probiotic Bacteria (<i>Lactococcus lactis</i>, and <i>Lactiplantibacillus plantarum</i>) in Calcium Alginate HydrogelsMarko Vinceković0Luna Maslov Bandić1Fabijan Oštarić2Marta Kiš3Nevijo Zdolec4Ivan Marić5Suzana Šegota6Hana Zelić7Nataša Mikulec8Department of Chemistry, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, CroatiaDepartment of Chemistry, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, CroatiaDepartment of Dairy Science, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, CroatiaLaboratory for Information Systems, Division of Electronics, Ruđer Bošković Institute, Bijenička c. 54, 10000 Zagreb, CroatiaLaboratory for Biocolloids and Surface Chemistry, Ruđer Bošković Institute, Bijenička c. 54, 10000 Zagreb, CroatiaDepartment of Chemistry, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, CroatiaDepartment of Dairy Science, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, CroatiaEncapsulation in alginate hydrogel microspheres is an effective method for protecting and improving the survival of lactic acid bacteria in different environments. This research aims to expand the knowledge about the structure/property relationship of calcium alginate microspheres loaded with a mixture of autochthonous probiotic bacteria (<i>Lactococcus lactis</i> and <i>Lactiplantibacillus plantarum</i>). A novel hydrogel formulation (FORMLAB) was prepared by ionic gelation and the molecular interactions between the FORMLAB constituents, surface morphology, structure, swelling degree, and release profile were characterized. The simultaneous encapsulation of two bacterial cultures in the same compartment does not diminish their viability. The binding of calcium ions to bacterial cells creates favorable conditions for the propagation of the encapsulated bacteria. The molecular interactions between the FORMLAB constituents are complex, involving mainly hydrogen bonds and electrostatic interactions. With a very high degree of swelling followed by low crosslinking, the surface of the microspheres covered with bacterial cells and diffusion through the hydrogel matrix allow for the delivery of probiotics at the right time. The findings suggest that bacterial cells are efficiently delivered from calcium alginate microspheres, offering promising applications in the development of functional foods, especially in cheese production.https://www.mdpi.com/2310-2861/11/1/34simultaneous encapsulation<i>Lactococcus lactis</i><i>Lactiplantibacillus plantarum</i>calcium alginate hydrogel |
| spellingShingle | Marko Vinceković Luna Maslov Bandić Fabijan Oštarić Marta Kiš Nevijo Zdolec Ivan Marić Suzana Šegota Hana Zelić Nataša Mikulec Simultaneous Encapsulation of Probiotic Bacteria (<i>Lactococcus lactis</i>, and <i>Lactiplantibacillus plantarum</i>) in Calcium Alginate Hydrogels Gels simultaneous encapsulation <i>Lactococcus lactis</i> <i>Lactiplantibacillus plantarum</i> calcium alginate hydrogel |
| title | Simultaneous Encapsulation of Probiotic Bacteria (<i>Lactococcus lactis</i>, and <i>Lactiplantibacillus plantarum</i>) in Calcium Alginate Hydrogels |
| title_full | Simultaneous Encapsulation of Probiotic Bacteria (<i>Lactococcus lactis</i>, and <i>Lactiplantibacillus plantarum</i>) in Calcium Alginate Hydrogels |
| title_fullStr | Simultaneous Encapsulation of Probiotic Bacteria (<i>Lactococcus lactis</i>, and <i>Lactiplantibacillus plantarum</i>) in Calcium Alginate Hydrogels |
| title_full_unstemmed | Simultaneous Encapsulation of Probiotic Bacteria (<i>Lactococcus lactis</i>, and <i>Lactiplantibacillus plantarum</i>) in Calcium Alginate Hydrogels |
| title_short | Simultaneous Encapsulation of Probiotic Bacteria (<i>Lactococcus lactis</i>, and <i>Lactiplantibacillus plantarum</i>) in Calcium Alginate Hydrogels |
| title_sort | simultaneous encapsulation of probiotic bacteria i lactococcus lactis i and i lactiplantibacillus plantarum i in calcium alginate hydrogels |
| topic | simultaneous encapsulation <i>Lactococcus lactis</i> <i>Lactiplantibacillus plantarum</i> calcium alginate hydrogel |
| url | https://www.mdpi.com/2310-2861/11/1/34 |
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