Isolation and characterization of acid- and Al-tolerant microorganisms
Acid- and aluminum (Al)-tolerant microorganisms were isolated from acidic tea fields, from which three fungal strains were selected and identified as Aspergillus zonatus, Penicillium sp. and Rhizopus sp. by molecular phylogenetic trees based on 18S rDNA gene sequences and morphological characteristi...
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Zhejiang University Press
2007-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.06.0626 |
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| author | GE De-yong YAO Huai-ying HUANG Chang-yong |
| author_facet | GE De-yong YAO Huai-ying HUANG Chang-yong |
| author_sort | GE De-yong |
| collection | DOAJ |
| description | Acid- and aluminum (Al)-tolerant microorganisms were isolated from acidic tea fields, from which three fungal strains were selected and identified as Aspergillus zonatus, Penicillium sp. and Rhizopus sp. by molecular phylogenetic trees based on 18S rDNA gene sequences and morphological characteristic analysis. They were tolerant to Al<sup>3+</sup> up to 100-200 mmol·L<sup>-1</sup> and could grow at low pH. F<sub>2</sub> strain even could grow at pH2.2, 200 mmol·L<sup>-1</sup> Al<sup>3+</sup>. Generally, the growth rates showed decreasing trends with the increasing concentration of Al<sup>3+</sup> when the microorganisms were cultured on agar plate with pH3.5. However, the low concentration of Al<sup>3+</sup> may accelerate the growth of F<sub>2</sub> strain. In a soil extract medium (pH3.5), pH of the spent medium of all strains increased to around 7.0 and Al<sup>3+</sup> content in the spent medium decreased due to the increase of pH. When put 1.0% glucose into the soil extract medium, pH of the spent medium decreased to 2.7, Al<sup>3+</sup> content in the spent medium almost remained constant. These results suggested that the fungi isolated possibly have different acid- and Al-tolerant mechanisms. |
| format | Article |
| id | doaj-art-58cc978b408c4276b6216d627b177780 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2007-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-58cc978b408c4276b6216d627b1777802025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552007-11-013362663210.3785/1008-9209.2007.06.062610089209Isolation and characterization of acid- and Al-tolerant microorganismsGE De-yongYAO Huai-yingHUANG Chang-yongAcid- and aluminum (Al)-tolerant microorganisms were isolated from acidic tea fields, from which three fungal strains were selected and identified as Aspergillus zonatus, Penicillium sp. and Rhizopus sp. by molecular phylogenetic trees based on 18S rDNA gene sequences and morphological characteristic analysis. They were tolerant to Al<sup>3+</sup> up to 100-200 mmol·L<sup>-1</sup> and could grow at low pH. F<sub>2</sub> strain even could grow at pH2.2, 200 mmol·L<sup>-1</sup> Al<sup>3+</sup>. Generally, the growth rates showed decreasing trends with the increasing concentration of Al<sup>3+</sup> when the microorganisms were cultured on agar plate with pH3.5. However, the low concentration of Al<sup>3+</sup> may accelerate the growth of F<sub>2</sub> strain. In a soil extract medium (pH3.5), pH of the spent medium of all strains increased to around 7.0 and Al<sup>3+</sup> content in the spent medium decreased due to the increase of pH. When put 1.0% glucose into the soil extract medium, pH of the spent medium decreased to 2.7, Al<sup>3+</sup> content in the spent medium almost remained constant. These results suggested that the fungi isolated possibly have different acid- and Al-tolerant mechanisms.https://www.academax.com/doi/10.3785/1008-9209.2007.06.0626aluminum (Al)fungiisolation and identificationacid- and Al-tolerant microorganisms |
| spellingShingle | GE De-yong YAO Huai-ying HUANG Chang-yong Isolation and characterization of acid- and Al-tolerant microorganisms 浙江大学学报. 农业与生命科学版 aluminum (Al) fungi isolation and identification acid- and Al-tolerant microorganisms |
| title | Isolation and characterization of acid- and Al-tolerant microorganisms |
| title_full | Isolation and characterization of acid- and Al-tolerant microorganisms |
| title_fullStr | Isolation and characterization of acid- and Al-tolerant microorganisms |
| title_full_unstemmed | Isolation and characterization of acid- and Al-tolerant microorganisms |
| title_short | Isolation and characterization of acid- and Al-tolerant microorganisms |
| title_sort | isolation and characterization of acid and al tolerant microorganisms |
| topic | aluminum (Al) fungi isolation and identification acid- and Al-tolerant microorganisms |
| url | https://www.academax.com/doi/10.3785/1008-9209.2007.06.0626 |
| work_keys_str_mv | AT gedeyong isolationandcharacterizationofacidandaltolerantmicroorganisms AT yaohuaiying isolationandcharacterizationofacidandaltolerantmicroorganisms AT huangchangyong isolationandcharacterizationofacidandaltolerantmicroorganisms |