Nanoporosity of Alumina Surfaces Induces Different Patterns of Activation in Adhering Monocytes/Macrophages
The present study shows that alumina nanotopography affects monocyte/macrophage behavior. Human mononuclear cells cultured on alumina membranes with pore diameters of 20 and 200 nm were evaluated in terms of cell adhesion, viability, morphology, and release of proinflammatory cytokines. After 24 hou...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2010/402715 |
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| author | Natalia Ferraz Jaan Hong Matteo Santin Marjam Karlsson Ott |
| author_facet | Natalia Ferraz Jaan Hong Matteo Santin Marjam Karlsson Ott |
| author_sort | Natalia Ferraz |
| collection | DOAJ |
| description | The present study shows that alumina nanotopography affects monocyte/macrophage behavior. Human mononuclear cells cultured on alumina membranes with pore diameters of 20 and 200 nm were evaluated in terms of cell adhesion, viability, morphology, and release of proinflammatory cytokines. After 24 hours, cell adhesion was assessed by means of light microscopy and cell viability by measuring LDH release. The inflammatory response was evaluated by quantifying interleukin-1β and tumour necrosis factor-α. Finally, scanning electron microscopy was used to study cell morphology. Results showed pronounced differences in cell number, morphology, and cytokine release depending on the nanoporosity. Few but highly activated cells were found on the 200 nm porous alumina, while relatively larger number of cells were found on the 20 nm porous surface. However, despite their larger number, the cells adhering on the 20 nm surface exhibited reduced pro-inflammatory activity. The data of this paper implies that nanotopography could be exploited for controlling the inflammatory response to implants. |
| format | Article |
| id | doaj-art-58c6b7789b334f30a3d5871aac231d3d |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-58c6b7789b334f30a3d5871aac231d3d2025-02-03T06:44:42ZengWileyInternational Journal of Biomaterials1687-87871687-87952010-01-01201010.1155/2010/402715402715Nanoporosity of Alumina Surfaces Induces Different Patterns of Activation in Adhering Monocytes/MacrophagesNatalia Ferraz0Jaan Hong1Matteo Santin2Marjam Karlsson Ott3Division of Surface Biotechnology, Department of Physical and Analytical Chemistry, BMC, Uppsala University, P.O. Box 577, 751 23 Uppsala, SwedenDivision of Clinical Immunology, Rudbeck Laboratory, Department of Oncology, Radiology and Clinical Immunology, University Hospital, Uppsala University, 751 85 Uppsala, SwedenSchool of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton BN2 4GJ, UKDivision of Surface Biotechnology, Department of Physical and Analytical Chemistry, BMC, Uppsala University, P.O. Box 577, 751 23 Uppsala, SwedenThe present study shows that alumina nanotopography affects monocyte/macrophage behavior. Human mononuclear cells cultured on alumina membranes with pore diameters of 20 and 200 nm were evaluated in terms of cell adhesion, viability, morphology, and release of proinflammatory cytokines. After 24 hours, cell adhesion was assessed by means of light microscopy and cell viability by measuring LDH release. The inflammatory response was evaluated by quantifying interleukin-1β and tumour necrosis factor-α. Finally, scanning electron microscopy was used to study cell morphology. Results showed pronounced differences in cell number, morphology, and cytokine release depending on the nanoporosity. Few but highly activated cells were found on the 200 nm porous alumina, while relatively larger number of cells were found on the 20 nm porous surface. However, despite their larger number, the cells adhering on the 20 nm surface exhibited reduced pro-inflammatory activity. The data of this paper implies that nanotopography could be exploited for controlling the inflammatory response to implants.http://dx.doi.org/10.1155/2010/402715 |
| spellingShingle | Natalia Ferraz Jaan Hong Matteo Santin Marjam Karlsson Ott Nanoporosity of Alumina Surfaces Induces Different Patterns of Activation in Adhering Monocytes/Macrophages International Journal of Biomaterials |
| title | Nanoporosity of Alumina Surfaces Induces Different Patterns of Activation in Adhering Monocytes/Macrophages |
| title_full | Nanoporosity of Alumina Surfaces Induces Different Patterns of Activation in Adhering Monocytes/Macrophages |
| title_fullStr | Nanoporosity of Alumina Surfaces Induces Different Patterns of Activation in Adhering Monocytes/Macrophages |
| title_full_unstemmed | Nanoporosity of Alumina Surfaces Induces Different Patterns of Activation in Adhering Monocytes/Macrophages |
| title_short | Nanoporosity of Alumina Surfaces Induces Different Patterns of Activation in Adhering Monocytes/Macrophages |
| title_sort | nanoporosity of alumina surfaces induces different patterns of activation in adhering monocytes macrophages |
| url | http://dx.doi.org/10.1155/2010/402715 |
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