Application of green silver nano-particles as anti-bacterial and photo-catalytic degradation of azo dye in wastewater
Abstract Ensuring everyone enjoys healthy lifestyles and well-being at all ages, Progress has been made in increasing access to clean water and sanitation facilities and reducing the spread of epidemics and diseases. The synthesis of nano-particles (NPs) by using microalgae is a new nanobiotechnolog...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-76090-w |
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| author | Zainab Haider Ali Lubna Abdulazeem Wurood Alwan Kadhim¹ Mazin H. Kzar³ Osamah J. Al-sareji |
| author_facet | Zainab Haider Ali Lubna Abdulazeem Wurood Alwan Kadhim¹ Mazin H. Kzar³ Osamah J. Al-sareji |
| author_sort | Zainab Haider Ali |
| collection | DOAJ |
| description | Abstract Ensuring everyone enjoys healthy lifestyles and well-being at all ages, Progress has been made in increasing access to clean water and sanitation facilities and reducing the spread of epidemics and diseases. The synthesis of nano-particles (NPs) by using microalgae is a new nanobiotechnology due to the use of the biomolecular (corona) of microalgae as a capping and reducing agent for NP creation. This investigation explores the capacity of a distinct indigenous microalgal strain to synthesize silver nano-particles (AgNPs), as well as its effectiveness against multi-drug resistant (MDR) bacteria and its ability to degrade Azo dye (Methyl Red) in wastewater. An extract of Spirulina platensis was obtained from a local source to synthesize silver nano-particles (AgNPs). The synthesized AgNPs were subsequently subjected to characterization utilizing several analytical methods, namely UV-visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction, and Fourier transform infrared spectroscopy (FTIR analysis). Subsequently, the disc diffusion method assessed their anti-bacterial efficacy against multi-drug resistant (MDR) bacteria and their ability to degrade Azo dye (Methyl Red) in wastewater. The nano-particles produced through biological synthesis exhibited a prominent peak in the UV-visible spectrum at a wavelength of 430 nm. Furthermore, these nano-particles were determined to possess a crystalline nature, with an average size of 28.72 nm and a distinctive star-like shape. The synthesized silver nano-particles (AgNPs) exhibited a dose-dependent anti-bacterial effect against some clinical bacterial isolates as multi-drug resistant (MDR), including Gram− ve bacteria such as Pseudomonas aeruginosa and Escherichia coli, as well as Gram+ ve bacteria like Staphylococcus aureus and Streptococcus pneumoniae. The action can be ascribed to the unique biological and physicochemical features of AgNPs, which facilitate the disruption of bacterial cell membranes. The UV-visible analysis solution after the introduction of AgNPs indicated that the decrease in the absorbance peak of methyl red was attributed to the existence of silver nano-particles. Metal nano-particles can be synthesized using environmentally friendly processes and hold great potential for combating multi-drug resistant bacteria and degrading Azo dyes. Silver nano-particles (AgNPs) are synthesized with an extract derived from the algae Spirulina platensis, which is a sustainable and eco-friendly alternative. |
| format | Article |
| id | doaj-art-58bec645e1c8412faab35a7162f272a3 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-58bec645e1c8412faab35a7162f272a32025-01-05T12:24:25ZengNature PortfolioScientific Reports2045-23222024-12-0114111210.1038/s41598-024-76090-wApplication of green silver nano-particles as anti-bacterial and photo-catalytic degradation of azo dye in wastewaterZainab Haider Ali0Lubna Abdulazeem1Wurood Alwan Kadhim¹2Mazin H. Kzar³3Osamah J. Al-sareji4Department of Biology, College of Science for Women, University of BabylonDNA Research Center, University of BabylonDepartment of Biology, College of Science for Women, University of BabylonCollege of Physical Education and Sport Sciences, Al-Mustaqbal UniversitySustainability Solutions Research Lab, Faculty of Engineering, University of PannoniaAbstract Ensuring everyone enjoys healthy lifestyles and well-being at all ages, Progress has been made in increasing access to clean water and sanitation facilities and reducing the spread of epidemics and diseases. The synthesis of nano-particles (NPs) by using microalgae is a new nanobiotechnology due to the use of the biomolecular (corona) of microalgae as a capping and reducing agent for NP creation. This investigation explores the capacity of a distinct indigenous microalgal strain to synthesize silver nano-particles (AgNPs), as well as its effectiveness against multi-drug resistant (MDR) bacteria and its ability to degrade Azo dye (Methyl Red) in wastewater. An extract of Spirulina platensis was obtained from a local source to synthesize silver nano-particles (AgNPs). The synthesized AgNPs were subsequently subjected to characterization utilizing several analytical methods, namely UV-visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction, and Fourier transform infrared spectroscopy (FTIR analysis). Subsequently, the disc diffusion method assessed their anti-bacterial efficacy against multi-drug resistant (MDR) bacteria and their ability to degrade Azo dye (Methyl Red) in wastewater. The nano-particles produced through biological synthesis exhibited a prominent peak in the UV-visible spectrum at a wavelength of 430 nm. Furthermore, these nano-particles were determined to possess a crystalline nature, with an average size of 28.72 nm and a distinctive star-like shape. The synthesized silver nano-particles (AgNPs) exhibited a dose-dependent anti-bacterial effect against some clinical bacterial isolates as multi-drug resistant (MDR), including Gram− ve bacteria such as Pseudomonas aeruginosa and Escherichia coli, as well as Gram+ ve bacteria like Staphylococcus aureus and Streptococcus pneumoniae. The action can be ascribed to the unique biological and physicochemical features of AgNPs, which facilitate the disruption of bacterial cell membranes. The UV-visible analysis solution after the introduction of AgNPs indicated that the decrease in the absorbance peak of methyl red was attributed to the existence of silver nano-particles. Metal nano-particles can be synthesized using environmentally friendly processes and hold great potential for combating multi-drug resistant bacteria and degrading Azo dyes. Silver nano-particles (AgNPs) are synthesized with an extract derived from the algae Spirulina platensis, which is a sustainable and eco-friendly alternative.https://doi.org/10.1038/s41598-024-76090-wSpirulina platensisAgNPsBiosynthetic NPsAnti-bacterialAzo dye degradation |
| spellingShingle | Zainab Haider Ali Lubna Abdulazeem Wurood Alwan Kadhim¹ Mazin H. Kzar³ Osamah J. Al-sareji Application of green silver nano-particles as anti-bacterial and photo-catalytic degradation of azo dye in wastewater Scientific Reports Spirulina platensis AgNPs Biosynthetic NPs Anti-bacterial Azo dye degradation |
| title | Application of green silver nano-particles as anti-bacterial and photo-catalytic degradation of azo dye in wastewater |
| title_full | Application of green silver nano-particles as anti-bacterial and photo-catalytic degradation of azo dye in wastewater |
| title_fullStr | Application of green silver nano-particles as anti-bacterial and photo-catalytic degradation of azo dye in wastewater |
| title_full_unstemmed | Application of green silver nano-particles as anti-bacterial and photo-catalytic degradation of azo dye in wastewater |
| title_short | Application of green silver nano-particles as anti-bacterial and photo-catalytic degradation of azo dye in wastewater |
| title_sort | application of green silver nano particles as anti bacterial and photo catalytic degradation of azo dye in wastewater |
| topic | Spirulina platensis AgNPs Biosynthetic NPs Anti-bacterial Azo dye degradation |
| url | https://doi.org/10.1038/s41598-024-76090-w |
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