Protein hydrolysates of niosome-encapsulated skipjack (Katsuwonus pelamis) viscera: Antioxidant, anticancer, and release properties
Skipjack (Katsuwonus pelamis) is one of the main tuna fish varieties in the canning industry. Approximately 50% of processed fish, particularly the viscera, which contains valuable compounds, is generated as waste with limited use. Recovering these bioactive compounds is a sustainable approach that...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Applied Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772502225000435 |
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| author | Shima Kaveh Alireza Sadeghi Mahoonak Vahid Erfani-Moghadam Mohammad Ghorbani Aliakbar Gholamhosseinpour Mojtaba Raeisi |
| author_facet | Shima Kaveh Alireza Sadeghi Mahoonak Vahid Erfani-Moghadam Mohammad Ghorbani Aliakbar Gholamhosseinpour Mojtaba Raeisi |
| author_sort | Shima Kaveh |
| collection | DOAJ |
| description | Skipjack (Katsuwonus pelamis) is one of the main tuna fish varieties in the canning industry. Approximately 50% of processed fish, particularly the viscera, which contains valuable compounds, is generated as waste with limited use. Recovering these bioactive compounds is a sustainable approach that can provide an excellent source of high-added value compounds, benefit the economy, and reduce environmental pollution. However, the wide application of these compounds in pharmaceutical and food fields is hindered due to their physical and chemical instability and uncontrolled delivery. So, this study aimed to produce skipjack viscera protein hydrolysates (FPH) using different enzymes (pancreatin, alcalase, trypsin, and pepsin) at different hydrolysis times. Additionally, the study investigated the stabilization of FPH by partially replacing cholesterol with cholecalciferol and squalane in nano-sized niosomes. The FPH produced by pancreatin at 200 min of hydrolysis exhibited the highest antioxidant and anticancer effects. These FPHs were then loaded into niosomes containing different ratios of stabilizers. The niosome formulation containing cholesterol, cholecalciferol, and squalane at a ratio of 2:1:1 achieved favorable encapsulation efficiency (91.8%) and retained total antioxidant capacity during simulated gastrointestinal fluids. The niosomes were observed to be spherical with smooth surfaces based on FE-SEM results, and successful loading of FPH in niosomes was confirmed by FTIR. As a result, niosomes containing cholecalciferol and squalane are stable carriers for the controlled release of bioactive drugs, including FPH as a high-added value product. |
| format | Article |
| id | doaj-art-58be97d49050455c808bec6b01e4d293 |
| institution | Kabale University |
| issn | 2772-5022 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Food Research |
| spelling | doaj-art-58be97d49050455c808bec6b01e4d2932025-02-03T04:17:07ZengElsevierApplied Food Research2772-50222025-06-0151100733Protein hydrolysates of niosome-encapsulated skipjack (Katsuwonus pelamis) viscera: Antioxidant, anticancer, and release propertiesShima Kaveh0Alireza Sadeghi Mahoonak1Vahid Erfani-Moghadam2Mohammad Ghorbani3Aliakbar Gholamhosseinpour4Mojtaba Raeisi5Faculty of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, 4918943464, IranFaculty of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, 4918943464, Iran; Corresponding author.Food, Drug, and Natural Products Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, IranFaculty of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, 4918943464, IranDepartment of Food Science and Technology, Faculty of Agriculture, Jahrom University, Jahrom, IranFood, Drug, and Natural Products Health Research Center, Golestan University of Medical Sciences, Gorgan, IranSkipjack (Katsuwonus pelamis) is one of the main tuna fish varieties in the canning industry. Approximately 50% of processed fish, particularly the viscera, which contains valuable compounds, is generated as waste with limited use. Recovering these bioactive compounds is a sustainable approach that can provide an excellent source of high-added value compounds, benefit the economy, and reduce environmental pollution. However, the wide application of these compounds in pharmaceutical and food fields is hindered due to their physical and chemical instability and uncontrolled delivery. So, this study aimed to produce skipjack viscera protein hydrolysates (FPH) using different enzymes (pancreatin, alcalase, trypsin, and pepsin) at different hydrolysis times. Additionally, the study investigated the stabilization of FPH by partially replacing cholesterol with cholecalciferol and squalane in nano-sized niosomes. The FPH produced by pancreatin at 200 min of hydrolysis exhibited the highest antioxidant and anticancer effects. These FPHs were then loaded into niosomes containing different ratios of stabilizers. The niosome formulation containing cholesterol, cholecalciferol, and squalane at a ratio of 2:1:1 achieved favorable encapsulation efficiency (91.8%) and retained total antioxidant capacity during simulated gastrointestinal fluids. The niosomes were observed to be spherical with smooth surfaces based on FE-SEM results, and successful loading of FPH in niosomes was confirmed by FTIR. As a result, niosomes containing cholecalciferol and squalane are stable carriers for the controlled release of bioactive drugs, including FPH as a high-added value product.http://www.sciencedirect.com/science/article/pii/S2772502225000435AnticancerAntioxidantNanoniosomeProtein hydrolysateSkipjack fish viscera |
| spellingShingle | Shima Kaveh Alireza Sadeghi Mahoonak Vahid Erfani-Moghadam Mohammad Ghorbani Aliakbar Gholamhosseinpour Mojtaba Raeisi Protein hydrolysates of niosome-encapsulated skipjack (Katsuwonus pelamis) viscera: Antioxidant, anticancer, and release properties Applied Food Research Anticancer Antioxidant Nanoniosome Protein hydrolysate Skipjack fish viscera |
| title | Protein hydrolysates of niosome-encapsulated skipjack (Katsuwonus pelamis) viscera: Antioxidant, anticancer, and release properties |
| title_full | Protein hydrolysates of niosome-encapsulated skipjack (Katsuwonus pelamis) viscera: Antioxidant, anticancer, and release properties |
| title_fullStr | Protein hydrolysates of niosome-encapsulated skipjack (Katsuwonus pelamis) viscera: Antioxidant, anticancer, and release properties |
| title_full_unstemmed | Protein hydrolysates of niosome-encapsulated skipjack (Katsuwonus pelamis) viscera: Antioxidant, anticancer, and release properties |
| title_short | Protein hydrolysates of niosome-encapsulated skipjack (Katsuwonus pelamis) viscera: Antioxidant, anticancer, and release properties |
| title_sort | protein hydrolysates of niosome encapsulated skipjack katsuwonus pelamis viscera antioxidant anticancer and release properties |
| topic | Anticancer Antioxidant Nanoniosome Protein hydrolysate Skipjack fish viscera |
| url | http://www.sciencedirect.com/science/article/pii/S2772502225000435 |
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