Potential of using transcriptome sequencing data in choosing therapy for patients with colorectal cancer

Aim. To study the applicability of RNA (ribonucleic acid) sequencing with master regulator identification for predicting the effectiveness of targeted therapy in patients with colorectal cancer.Materials and methods. Tissue samples from three patients with colorectal cancer obtained from postoperati...

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Main Authors: S. V. Apalko, V. S. Shimansky, A. Kel, N. N. Sushentseva, N. V. Tyukavina, S. A. Kovalenko, S. G. Shcherbak
Format: Article
Language:Russian
Published: «SILICEA-POLIGRAF» LLC 2025-01-01
Series:Кардиоваскулярная терапия и профилактика
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Online Access:https://cardiovascular.elpub.ru/jour/article/view/4174
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author S. V. Apalko
V. S. Shimansky
A. Kel
N. N. Sushentseva
N. V. Tyukavina
S. A. Kovalenko
S. G. Shcherbak
author_facet S. V. Apalko
V. S. Shimansky
A. Kel
N. N. Sushentseva
N. V. Tyukavina
S. A. Kovalenko
S. G. Shcherbak
author_sort S. V. Apalko
collection DOAJ
description Aim. To study the applicability of RNA (ribonucleic acid) sequencing with master regulator identification for predicting the effectiveness of targeted therapy in patients with colorectal cancer.Materials and methods. Tissue samples from three patients with colorectal cancer obtained from postoperative material were used. All patients received palliative antitumor therapy in standard regimens in accordance with the tumor location and the status of RAS and BRAF gene mutations. The transcriptome of tumor and healthy tissue of each patient was sequenced, and master regulators in the tumor tissue were analyzed.Results. A list of master regulators was found for each patient and possible therapeutic agents most suitable for suppressing the tumor process were predicted.Conclusion. The potential of computer analysis of the molecular profile of colorectal adenocarcinoma in predicting the effectiveness of therapy is shown. However, to determine the clinical potential of this technique, a study on a wider sample is required.
format Article
id doaj-art-58b5abb2e04047e5a66909a7fbe6c4b1
institution Kabale University
issn 1728-8800
2619-0125
language Russian
publishDate 2025-01-01
publisher «SILICEA-POLIGRAF» LLC
record_format Article
series Кардиоваскулярная терапия и профилактика
spelling doaj-art-58b5abb2e04047e5a66909a7fbe6c4b12025-08-20T03:57:18Zrus«SILICEA-POLIGRAF» LLCКардиоваскулярная терапия и профилактика1728-88002619-01252025-01-01231110.15829/1728-8800-2024-41743051Potential of using transcriptome sequencing data in choosing therapy for patients with colorectal cancerS. V. Apalko0V. S. Shimansky1A. Kel2N. N. Sushentseva3N. V. Tyukavina4S. A. Kovalenko5S. G. Shcherbak6Kurortny District Сity Hospital № 40; Saint Petersburg State UniversityKurortny District Сity Hospital № 40; Saint Petersburg State UniversitygeneXplain GmbHKurortny District Сity Hospital № 40; Saint Petersburg State UniversityKurortny District Сity Hospital № 40Kurortny District Сity Hospital № 40Kurortny District Сity Hospital № 40; Saint Petersburg State UniversityAim. To study the applicability of RNA (ribonucleic acid) sequencing with master regulator identification for predicting the effectiveness of targeted therapy in patients with colorectal cancer.Materials and methods. Tissue samples from three patients with colorectal cancer obtained from postoperative material were used. All patients received palliative antitumor therapy in standard regimens in accordance with the tumor location and the status of RAS and BRAF gene mutations. The transcriptome of tumor and healthy tissue of each patient was sequenced, and master regulators in the tumor tissue were analyzed.Results. A list of master regulators was found for each patient and possible therapeutic agents most suitable for suppressing the tumor process were predicted.Conclusion. The potential of computer analysis of the molecular profile of colorectal adenocarcinoma in predicting the effectiveness of therapy is shown. However, to determine the clinical potential of this technique, a study on a wider sample is required.https://cardiovascular.elpub.ru/jour/article/view/4174rna sequencingcolorectal cancertranscriptomemaster regulatorstargeted therapy
spellingShingle S. V. Apalko
V. S. Shimansky
A. Kel
N. N. Sushentseva
N. V. Tyukavina
S. A. Kovalenko
S. G. Shcherbak
Potential of using transcriptome sequencing data in choosing therapy for patients with colorectal cancer
Кардиоваскулярная терапия и профилактика
rna sequencing
colorectal cancer
transcriptome
master regulators
targeted therapy
title Potential of using transcriptome sequencing data in choosing therapy for patients with colorectal cancer
title_full Potential of using transcriptome sequencing data in choosing therapy for patients with colorectal cancer
title_fullStr Potential of using transcriptome sequencing data in choosing therapy for patients with colorectal cancer
title_full_unstemmed Potential of using transcriptome sequencing data in choosing therapy for patients with colorectal cancer
title_short Potential of using transcriptome sequencing data in choosing therapy for patients with colorectal cancer
title_sort potential of using transcriptome sequencing data in choosing therapy for patients with colorectal cancer
topic rna sequencing
colorectal cancer
transcriptome
master regulators
targeted therapy
url https://cardiovascular.elpub.ru/jour/article/view/4174
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