Microneedles for Continuous, Minimally Invasive Monitoring: A Technology Overview

Abstract Microneedles are small piercing structures, with sizes in the micron‐to‐millimeter range, designed to penetrate painlessly the outer skin layer, known as stratum corneum, providing minimally invasive access to interstitial fluid (ISF), which enables the monitoring of biochemical parameters...

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Main Authors: Guillermo Conejo‐Cuevas, Miguel Aller Pellitero, Leire Ruiz‐Rubio, Francisco Javier del Campo
Format: Article
Language:English
Published: Wiley-VCH 2025-07-01
Series:Advanced Sensor Research
Subjects:
Online Access:https://doi.org/10.1002/adsr.202500057
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author Guillermo Conejo‐Cuevas
Miguel Aller Pellitero
Leire Ruiz‐Rubio
Francisco Javier del Campo
author_facet Guillermo Conejo‐Cuevas
Miguel Aller Pellitero
Leire Ruiz‐Rubio
Francisco Javier del Campo
author_sort Guillermo Conejo‐Cuevas
collection DOAJ
description Abstract Microneedles are small piercing structures, with sizes in the micron‐to‐millimeter range, designed to penetrate painlessly the outer skin layer, known as stratum corneum, providing minimally invasive access to interstitial fluid (ISF), which enables the monitoring of biochemical parameters in real‐time. This review covers recent progress to date in the area of electrochemical sensing using microneedles, and provides an overview of fabrication materials and processes, as well as applications. The main body of the review focuses on the fabrication of microneedle structures and their transformation into electrochemical biosensors for continuous monitoring. To this end, the main recognition elements and electrode functionalization ways are described, paying closer attention to aptamers and continuous aptamer‐based sensing, whose importance, merits, and limitations are highlighted. In addition to covering the main current applications, the review discusses the future threats and opportunities of microneedle‐based in vivo monitoring.
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institution Kabale University
issn 2751-1219
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series Advanced Sensor Research
spelling doaj-art-58a931c356f94d7aa25ead58e2789dbe2025-08-20T03:49:49ZengWiley-VCHAdvanced Sensor Research2751-12192025-07-0147n/an/a10.1002/adsr.202500057Microneedles for Continuous, Minimally Invasive Monitoring: A Technology OverviewGuillermo Conejo‐Cuevas0Miguel Aller Pellitero1Leire Ruiz‐Rubio2Francisco Javier del Campo3BCMaterials Basque Center for Materials Applications and Nanostructures UPV/EHU Science Park Leioa Vizcaya 48940 SpainDepartamento de Química Física y Analítica Universidad de Oviedo Av. Julián Clavería 8 Oviedo 33006 SpainBCMaterials Basque Center for Materials Applications and Nanostructures UPV/EHU Science Park Leioa Vizcaya 48940 SpainBCMaterials Basque Center for Materials Applications and Nanostructures UPV/EHU Science Park Leioa Vizcaya 48940 SpainAbstract Microneedles are small piercing structures, with sizes in the micron‐to‐millimeter range, designed to penetrate painlessly the outer skin layer, known as stratum corneum, providing minimally invasive access to interstitial fluid (ISF), which enables the monitoring of biochemical parameters in real‐time. This review covers recent progress to date in the area of electrochemical sensing using microneedles, and provides an overview of fabrication materials and processes, as well as applications. The main body of the review focuses on the fabrication of microneedle structures and their transformation into electrochemical biosensors for continuous monitoring. To this end, the main recognition elements and electrode functionalization ways are described, paying closer attention to aptamers and continuous aptamer‐based sensing, whose importance, merits, and limitations are highlighted. In addition to covering the main current applications, the review discusses the future threats and opportunities of microneedle‐based in vivo monitoring.https://doi.org/10.1002/adsr.202500057aptamerselectrochemical sensorsinterstitial fluidmicroneedleswearable biosensors
spellingShingle Guillermo Conejo‐Cuevas
Miguel Aller Pellitero
Leire Ruiz‐Rubio
Francisco Javier del Campo
Microneedles for Continuous, Minimally Invasive Monitoring: A Technology Overview
Advanced Sensor Research
aptamers
electrochemical sensors
interstitial fluid
microneedles
wearable biosensors
title Microneedles for Continuous, Minimally Invasive Monitoring: A Technology Overview
title_full Microneedles for Continuous, Minimally Invasive Monitoring: A Technology Overview
title_fullStr Microneedles for Continuous, Minimally Invasive Monitoring: A Technology Overview
title_full_unstemmed Microneedles for Continuous, Minimally Invasive Monitoring: A Technology Overview
title_short Microneedles for Continuous, Minimally Invasive Monitoring: A Technology Overview
title_sort microneedles for continuous minimally invasive monitoring a technology overview
topic aptamers
electrochemical sensors
interstitial fluid
microneedles
wearable biosensors
url https://doi.org/10.1002/adsr.202500057
work_keys_str_mv AT guillermoconejocuevas microneedlesforcontinuousminimallyinvasivemonitoringatechnologyoverview
AT miguelallerpellitero microneedlesforcontinuousminimallyinvasivemonitoringatechnologyoverview
AT leireruizrubio microneedlesforcontinuousminimallyinvasivemonitoringatechnologyoverview
AT franciscojavierdelcampo microneedlesforcontinuousminimallyinvasivemonitoringatechnologyoverview