Use of calcium hydroxyapatite and growth factors in endodontic therapy
Background/Aim. Hydroxyapatite (HAp) is one of the most commonly used calcium phosphate bioceramics with osteoconductive properties. Growth factors are capable of directly inducing morphological and functional differentiation of neodontoblasts. The aim of this study was to investigate the effectiven...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2021-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900069M.pdf |
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| author | Mitić Aleksandar Živković Milan Živković Dušan Popović Lidija Veličković Zorana Miladinović Milan Šubarić Ljiljana Marjanović Dragan Cvetković Andrijana |
| author_facet | Mitić Aleksandar Živković Milan Živković Dušan Popović Lidija Veličković Zorana Miladinović Milan Šubarić Ljiljana Marjanović Dragan Cvetković Andrijana |
| author_sort | Mitić Aleksandar |
| collection | DOAJ |
| description | Background/Aim. Hydroxyapatite (HAp) is one of the most commonly used calcium phosphate bioceramics with osteoconductive properties. Growth factors are capable of directly inducing morphological and functional differentiation of neodontoblasts. The aim of this study was to investigate the effectiveness of HAp-based biomaterial in combination with transforming growth factor-β1 (TGF-β1) in the creation of new dentine and obturation of the root canal apex in the teeth of an experimental animal model. Methods. Rodent (rabbit) teeth were used as the experimental animal model. After pulp removal with a pulp extirpator in vital pulpectomy, the biomaterial was applied using a Lentulo spiral in the apex portion at the level of the physiological foramen apicale. The experiment was performed in general anesthesia. Animals were kept alive for 3, 6, and 12 months after the treatment. The extracted teeth were analyzed by scanning electron microscopy (SEM). Results. Using SEM, it was found that the number of teeth with newly created dentine and apex canal obturation was greater 12 months after the treatment. Conclusion. Apex obturation of the dental root canal with newly created dentine took place in our experimental groups treated by biomaterial with or without TGF-β1. |
| format | Article |
| id | doaj-art-58a92638c2174ca995911b3b9e86cc09 |
| institution | DOAJ |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-58a92638c2174ca995911b3b9e86cc092025-08-20T03:19:35ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202021-01-0178331031610.2298/VSP190405069M0042-84501900069MUse of calcium hydroxyapatite and growth factors in endodontic therapyMitić Aleksandar0Živković Milan1Živković Dušan2Popović Lidija3Veličković Zorana4Miladinović Milan5Šubarić Ljiljana6Marjanović Dragan7Cvetković Andrijana8University of Niš, Faculty of Medicine, Dental Clinic, Niš, SerbiaUniversity of Priština/Kosovska Mitrovica, Faculty of Medicine, Kosovska Mitrovica, SerbiaUniversity of Priština/Kosovska Mitrovica, Faculty of Medicine, Kosovska Mitrovica, SerbiaInstitute for Psychophysiological Disorders and Speech Pathology “Prof. Dr. Cvetko Brajović”, Belgrade, SerbiaUniversity of Priština/Kosovska Mitrovica, Faculty of Medicine, Kosovska Mitrovica, SerbiaUniversity of Priština/Kosovska Mitrovica, Faculty of Medicine, Kosovska Mitrovica, SerbiaUniversity of Priština/Kosovska Mitrovica, Faculty of Medicine, Kosovska Mitrovica, SerbiaUniversity of Priština/Kosovska Mitrovica, Faculty of Medicine, Kosovska Mitrovica, SerbiaUniversity of Priština/Kosovska Mitrovica, Faculty of Medicine, Kosovska Mitrovica, SerbiaBackground/Aim. Hydroxyapatite (HAp) is one of the most commonly used calcium phosphate bioceramics with osteoconductive properties. Growth factors are capable of directly inducing morphological and functional differentiation of neodontoblasts. The aim of this study was to investigate the effectiveness of HAp-based biomaterial in combination with transforming growth factor-β1 (TGF-β1) in the creation of new dentine and obturation of the root canal apex in the teeth of an experimental animal model. Methods. Rodent (rabbit) teeth were used as the experimental animal model. After pulp removal with a pulp extirpator in vital pulpectomy, the biomaterial was applied using a Lentulo spiral in the apex portion at the level of the physiological foramen apicale. The experiment was performed in general anesthesia. Animals were kept alive for 3, 6, and 12 months after the treatment. The extracted teeth were analyzed by scanning electron microscopy (SEM). Results. Using SEM, it was found that the number of teeth with newly created dentine and apex canal obturation was greater 12 months after the treatment. Conclusion. Apex obturation of the dental root canal with newly created dentine took place in our experimental groups treated by biomaterial with or without TGF-β1.http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900069M.pdfodontoblastsendodonticsdentinbiocompatible materialstransforming growth factorstooth rootrabbits |
| spellingShingle | Mitić Aleksandar Živković Milan Živković Dušan Popović Lidija Veličković Zorana Miladinović Milan Šubarić Ljiljana Marjanović Dragan Cvetković Andrijana Use of calcium hydroxyapatite and growth factors in endodontic therapy Vojnosanitetski Pregled odontoblasts endodontics dentin biocompatible materials transforming growth factors tooth root rabbits |
| title | Use of calcium hydroxyapatite and growth factors in endodontic therapy |
| title_full | Use of calcium hydroxyapatite and growth factors in endodontic therapy |
| title_fullStr | Use of calcium hydroxyapatite and growth factors in endodontic therapy |
| title_full_unstemmed | Use of calcium hydroxyapatite and growth factors in endodontic therapy |
| title_short | Use of calcium hydroxyapatite and growth factors in endodontic therapy |
| title_sort | use of calcium hydroxyapatite and growth factors in endodontic therapy |
| topic | odontoblasts endodontics dentin biocompatible materials transforming growth factors tooth root rabbits |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900069M.pdf |
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