Development of Biphasic Culture System for an Entomopathogenic Fungus <i>Beauveria bassiana</i> PfBb Strain and Its Virulence on a Defoliating Moth <i>Phauda flammans</i> (Walker)
<i>Beauveria bassiana</i> PfBb is a new strain with high host specificity to the target pest <i>Phauda flammans</i>. We conducted a series of experiments to optimize the biphasic fermentation system of <i>B. bassiana</i> PfBb by screening the medium compositions a...
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2025-03-01
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| author | Yi-Ping Gao De-Xiang Shi Yuan-Hao Li Xiong Zhao He Xiao-Yun Wang Kai Lin Xia-Lin Zheng |
| author_facet | Yi-Ping Gao De-Xiang Shi Yuan-Hao Li Xiong Zhao He Xiao-Yun Wang Kai Lin Xia-Lin Zheng |
| author_sort | Yi-Ping Gao |
| collection | DOAJ |
| description | <i>Beauveria bassiana</i> PfBb is a new strain with high host specificity to the target pest <i>Phauda flammans</i>. We conducted a series of experiments to optimize the biphasic fermentation system of <i>B. bassiana</i> PfBb by screening the medium compositions and fermentation environmental conditions in both liquid and solid fermentations. In the liquid fermentation, glucose and yeast extract with a C:N ratio of 17:1 were the optimal carbon and nitrogen sources, respectively, for <i>B. bassiana</i> PfBb mycelium growth and blastospore production, and liquid fermentation with an inoculation concentration of 1 × 10<sup>8</sup>/mL and an inoculum content of 50 mL conidial suspension, at 180 rpm/min rotation speed, pH 7 and 26 °C, favored mycelium growth. However, additional trace elements did not significantly improve liquid fermentation. In the solid fermentation, wheat bran and chaff at a ratio of 8:2 were identified as the best substrates that facilitated <i>B. bassiana</i> PfBb sporulation and conidial germination, and optimal substrates with 20% inoculum content, 50% water content, and 3-day fermentation in darkness had the highest conidia yield. The resulting conidia, stored at −20, 4, and 20 °C for one year, did not significantly change the water content, and with prolonged storage duration, conidial germination was significantly higher at −20 and 4 °C. Moreover, conidia stored at 4 °C for one year maintained its validity and virulence, which were toxic to all instar larvae of <i>P. flammans</i>. Our results provide essential support for the commercial production of <i>B. bassiana</i> PfBb-based biopesticides. |
| format | Article |
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| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-58a50670f7bb41ec8a37751cecac4aa92025-08-20T03:43:16ZengMDPI AGJournal of Fungi2309-608X2025-03-0111320210.3390/jof11030202Development of Biphasic Culture System for an Entomopathogenic Fungus <i>Beauveria bassiana</i> PfBb Strain and Its Virulence on a Defoliating Moth <i>Phauda flammans</i> (Walker)Yi-Ping Gao0De-Xiang Shi1Yuan-Hao Li2Xiong Zhao He3Xiao-Yun Wang4Kai Lin5Xia-Lin Zheng6Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, ChinaSchool of Agriculture and Environment, Massey University, Private Bag, Palmerston North 4410, New ZealandGuangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, China<i>Beauveria bassiana</i> PfBb is a new strain with high host specificity to the target pest <i>Phauda flammans</i>. We conducted a series of experiments to optimize the biphasic fermentation system of <i>B. bassiana</i> PfBb by screening the medium compositions and fermentation environmental conditions in both liquid and solid fermentations. In the liquid fermentation, glucose and yeast extract with a C:N ratio of 17:1 were the optimal carbon and nitrogen sources, respectively, for <i>B. bassiana</i> PfBb mycelium growth and blastospore production, and liquid fermentation with an inoculation concentration of 1 × 10<sup>8</sup>/mL and an inoculum content of 50 mL conidial suspension, at 180 rpm/min rotation speed, pH 7 and 26 °C, favored mycelium growth. However, additional trace elements did not significantly improve liquid fermentation. In the solid fermentation, wheat bran and chaff at a ratio of 8:2 were identified as the best substrates that facilitated <i>B. bassiana</i> PfBb sporulation and conidial germination, and optimal substrates with 20% inoculum content, 50% water content, and 3-day fermentation in darkness had the highest conidia yield. The resulting conidia, stored at −20, 4, and 20 °C for one year, did not significantly change the water content, and with prolonged storage duration, conidial germination was significantly higher at −20 and 4 °C. Moreover, conidia stored at 4 °C for one year maintained its validity and virulence, which were toxic to all instar larvae of <i>P. flammans</i>. Our results provide essential support for the commercial production of <i>B. bassiana</i> PfBb-based biopesticides.https://www.mdpi.com/2309-608X/11/3/202<i>Beauveria bassiana</i>fermentation technologymycelium growthsporulationconidial validity and virulenceprolonging storage |
| spellingShingle | Yi-Ping Gao De-Xiang Shi Yuan-Hao Li Xiong Zhao He Xiao-Yun Wang Kai Lin Xia-Lin Zheng Development of Biphasic Culture System for an Entomopathogenic Fungus <i>Beauveria bassiana</i> PfBb Strain and Its Virulence on a Defoliating Moth <i>Phauda flammans</i> (Walker) Journal of Fungi <i>Beauveria bassiana</i> fermentation technology mycelium growth sporulation conidial validity and virulence prolonging storage |
| title | Development of Biphasic Culture System for an Entomopathogenic Fungus <i>Beauveria bassiana</i> PfBb Strain and Its Virulence on a Defoliating Moth <i>Phauda flammans</i> (Walker) |
| title_full | Development of Biphasic Culture System for an Entomopathogenic Fungus <i>Beauveria bassiana</i> PfBb Strain and Its Virulence on a Defoliating Moth <i>Phauda flammans</i> (Walker) |
| title_fullStr | Development of Biphasic Culture System for an Entomopathogenic Fungus <i>Beauveria bassiana</i> PfBb Strain and Its Virulence on a Defoliating Moth <i>Phauda flammans</i> (Walker) |
| title_full_unstemmed | Development of Biphasic Culture System for an Entomopathogenic Fungus <i>Beauveria bassiana</i> PfBb Strain and Its Virulence on a Defoliating Moth <i>Phauda flammans</i> (Walker) |
| title_short | Development of Biphasic Culture System for an Entomopathogenic Fungus <i>Beauveria bassiana</i> PfBb Strain and Its Virulence on a Defoliating Moth <i>Phauda flammans</i> (Walker) |
| title_sort | development of biphasic culture system for an entomopathogenic fungus i beauveria bassiana i pfbb strain and its virulence on a defoliating moth i phauda flammans i walker |
| topic | <i>Beauveria bassiana</i> fermentation technology mycelium growth sporulation conidial validity and virulence prolonging storage |
| url | https://www.mdpi.com/2309-608X/11/3/202 |
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