Development of Biphasic Culture System for an Entomopathogenic Fungus <i>Beauveria bassiana</i> PfBb Strain and Its Virulence on a Defoliating Moth <i>Phauda flammans</i> (Walker)
<i>Beauveria bassiana</i> PfBb is a new strain with high host specificity to the target pest <i>Phauda flammans</i>. We conducted a series of experiments to optimize the biphasic fermentation system of <i>B. bassiana</i> PfBb by screening the medium compositions a...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
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| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/3/202 |
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| Summary: | <i>Beauveria bassiana</i> PfBb is a new strain with high host specificity to the target pest <i>Phauda flammans</i>. We conducted a series of experiments to optimize the biphasic fermentation system of <i>B. bassiana</i> PfBb by screening the medium compositions and fermentation environmental conditions in both liquid and solid fermentations. In the liquid fermentation, glucose and yeast extract with a C:N ratio of 17:1 were the optimal carbon and nitrogen sources, respectively, for <i>B. bassiana</i> PfBb mycelium growth and blastospore production, and liquid fermentation with an inoculation concentration of 1 × 10<sup>8</sup>/mL and an inoculum content of 50 mL conidial suspension, at 180 rpm/min rotation speed, pH 7 and 26 °C, favored mycelium growth. However, additional trace elements did not significantly improve liquid fermentation. In the solid fermentation, wheat bran and chaff at a ratio of 8:2 were identified as the best substrates that facilitated <i>B. bassiana</i> PfBb sporulation and conidial germination, and optimal substrates with 20% inoculum content, 50% water content, and 3-day fermentation in darkness had the highest conidia yield. The resulting conidia, stored at −20, 4, and 20 °C for one year, did not significantly change the water content, and with prolonged storage duration, conidial germination was significantly higher at −20 and 4 °C. Moreover, conidia stored at 4 °C for one year maintained its validity and virulence, which were toxic to all instar larvae of <i>P. flammans</i>. Our results provide essential support for the commercial production of <i>B. bassiana</i> PfBb-based biopesticides. |
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| ISSN: | 2309-608X |