Lead toxicity in Nicotiana tabacum L.: Damage antioxidant system and disturb plant metabolism
In this study, we treated tobacco seedlings with 0, 200, 400, and 800 mg/kg Pb2 +, and explored the response mechanism of tobacco under Pb stress through a combination of growth physiology and metabolomics analysis. The physiological results showed that compared with CK, with the increase of Pb conc...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325001733 |
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| author | Tengfei Liu Kai Zhang Chunlan Ming Jiashu Tian Huanyu Teng Zicheng Xu Jiewang He Fengfeng Liu Yinghui Zhou Jiayang Xu Mohamed G. Moussa Shenghua Zhang Wei Jia |
| author_facet | Tengfei Liu Kai Zhang Chunlan Ming Jiashu Tian Huanyu Teng Zicheng Xu Jiewang He Fengfeng Liu Yinghui Zhou Jiayang Xu Mohamed G. Moussa Shenghua Zhang Wei Jia |
| author_sort | Tengfei Liu |
| collection | DOAJ |
| description | In this study, we treated tobacco seedlings with 0, 200, 400, and 800 mg/kg Pb2 +, and explored the response mechanism of tobacco under Pb stress through a combination of growth physiology and metabolomics analysis. The physiological results showed that compared with CK, with the increase of Pb concentration, Pb treatment inhibited tobacco growth, reduced the biomass and photosynthetic pigment content of tobacco seedlings, and severely damaged the chloroplast structure. In addition, compared with CK, the pore conductivity and pore density of Pb800 treatment decreased by 45.77 % and 93.55 %, respectively. Pb treatment disrupted the cell membrane system, and Pb800 treatment increased the content of malondialdehyde (MDA) in leaves and roots by 67.65 % and 31.90 %, respectively. Meanwhile, Pb treatment increased the activity of tobacco SOD and POD enzymes. Metabolomics results showed that Pb stress enhanced tryptophan metabolism, glutathione metabolism, alanine, aspartate, and glutamate metabolism, as well as cysteine and methionine metabolism pathways. These results indicate that increasing the content of photosynthetic pigments and hormones, clearing reactive oxygen species by enhancing antioxidant enzyme activity, and improving amino acid metabolism may play an important role in reducing the toxicity of Pb to tobacco. |
| format | Article |
| id | doaj-art-58a3551465354a6da9a979b29fe4f8ed |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-58a3551465354a6da9a979b29fe4f8ed2025-02-09T04:59:37ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117837Lead toxicity in Nicotiana tabacum L.: Damage antioxidant system and disturb plant metabolismTengfei Liu0Kai Zhang1Chunlan Ming2Jiashu Tian3Huanyu Teng4Zicheng Xu5Jiewang He6Fengfeng Liu7Yinghui Zhou8Jiayang Xu9Mohamed G. Moussa10Shenghua Zhang11Wei Jia12National Tobacco Cultivation and Physiology and Biochemistry Research Center, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaNational Tobacco Cultivation and Physiology and Biochemistry Research Center, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaNational Tobacco Cultivation and Physiology and Biochemistry Research Center, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaNational Tobacco Cultivation and Physiology and Biochemistry Research Center, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaNational Tobacco Cultivation and Physiology and Biochemistry Research Center, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaNational Tobacco Cultivation and Physiology and Biochemistry Research Center, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaTechnology Center, China Tobacco Hubei Industrial Co., LTD, Wuhan 430040, ChinaTechnology Center, China Tobacco Hubei Industrial Co., LTD, Wuhan 430040, ChinaTechnology Center, China Tobacco Hubei Industrial Co., LTD, Wuhan 430040, ChinaCollege of Resources and Environment, Henan Agricultural University, Zhengzhou 450002, ChinaInternational Center for Biosaline Agriculture, ICBA, Dubai 14660, United Arab Emirates; Soil and Water Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo 13759, EgyptTechnology Center, China Tobacco Hubei Industrial Co., LTD, Wuhan 430040, China; Corresponding authors.National Tobacco Cultivation and Physiology and Biochemistry Research Center, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China; Corresponding authors.In this study, we treated tobacco seedlings with 0, 200, 400, and 800 mg/kg Pb2 +, and explored the response mechanism of tobacco under Pb stress through a combination of growth physiology and metabolomics analysis. The physiological results showed that compared with CK, with the increase of Pb concentration, Pb treatment inhibited tobacco growth, reduced the biomass and photosynthetic pigment content of tobacco seedlings, and severely damaged the chloroplast structure. In addition, compared with CK, the pore conductivity and pore density of Pb800 treatment decreased by 45.77 % and 93.55 %, respectively. Pb treatment disrupted the cell membrane system, and Pb800 treatment increased the content of malondialdehyde (MDA) in leaves and roots by 67.65 % and 31.90 %, respectively. Meanwhile, Pb treatment increased the activity of tobacco SOD and POD enzymes. Metabolomics results showed that Pb stress enhanced tryptophan metabolism, glutathione metabolism, alanine, aspartate, and glutamate metabolism, as well as cysteine and methionine metabolism pathways. These results indicate that increasing the content of photosynthetic pigments and hormones, clearing reactive oxygen species by enhancing antioxidant enzyme activity, and improving amino acid metabolism may play an important role in reducing the toxicity of Pb to tobacco.http://www.sciencedirect.com/science/article/pii/S0147651325001733PbPhysiologyToxicityMetabolomicsTobacco |
| spellingShingle | Tengfei Liu Kai Zhang Chunlan Ming Jiashu Tian Huanyu Teng Zicheng Xu Jiewang He Fengfeng Liu Yinghui Zhou Jiayang Xu Mohamed G. Moussa Shenghua Zhang Wei Jia Lead toxicity in Nicotiana tabacum L.: Damage antioxidant system and disturb plant metabolism Ecotoxicology and Environmental Safety Pb Physiology Toxicity Metabolomics Tobacco |
| title | Lead toxicity in Nicotiana tabacum L.: Damage antioxidant system and disturb plant metabolism |
| title_full | Lead toxicity in Nicotiana tabacum L.: Damage antioxidant system and disturb plant metabolism |
| title_fullStr | Lead toxicity in Nicotiana tabacum L.: Damage antioxidant system and disturb plant metabolism |
| title_full_unstemmed | Lead toxicity in Nicotiana tabacum L.: Damage antioxidant system and disturb plant metabolism |
| title_short | Lead toxicity in Nicotiana tabacum L.: Damage antioxidant system and disturb plant metabolism |
| title_sort | lead toxicity in nicotiana tabacum l damage antioxidant system and disturb plant metabolism |
| topic | Pb Physiology Toxicity Metabolomics Tobacco |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325001733 |
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