Impact of environmental and geographical position on the chemometric classification of ethanol extracts from Isotoma longiflora leaves

BACKGROUND AND OBJECTIVES: Isotoma longiflora L is commonly used as a medicinal plant by the local community in Indonesia, and its geographical position determines its bioactive compounds and hence its efficacy. Ethanol extracts of Isotoma longiflora leaves from various locations in Aceh Province we...

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Main Authors: E. Imelda, K. Khairan, R.R. Lubis, T. Karma, R. Idroes
Format: Article
Language:English
Published: GJESM Publisher 2024-01-01
Series:Global Journal of Environmental Science and Management
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Online Access:https://www.gjesm.net/article_705700_55a9d31fc3f2510cae6caf8643bd2e2b.pdf
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author E. Imelda
K. Khairan
R.R. Lubis
T. Karma
R. Idroes
author_facet E. Imelda
K. Khairan
R.R. Lubis
T. Karma
R. Idroes
author_sort E. Imelda
collection DOAJ
description BACKGROUND AND OBJECTIVES: Isotoma longiflora L is commonly used as a medicinal plant by the local community in Indonesia, and its geographical position determines its bioactive compounds and hence its efficacy. Ethanol extracts of Isotoma longiflora leaves from various locations in Aceh Province were analyzed using a simple infrared spectroscopy technique combined with chemometrics to determine the effect of geographical location and conditions by classification and authentication.METHODS: Isotoma longiflora leaf samples were collected from Aceh Besar (a geothermal manifestation of Ie Suum), Banda Aceh, Aceh Jaya, Bireun, and Central Aceh. Principal component analysis was used to categorize the ethanol extract of Isotoma longiflora leaves, and a linear discriminant analysis was used for authentication.FINDINGS: The principal component analysis score plot indicated 89 percent of total data variance and that the samples formed three distinct groups: group I consisting of Aceh Tengah and Bener Meriah samples; group II of Aceh Besar and Banda Aceh samples; and group III of Aceh Selatan, Aceh Barat Daya, Aceh Jaya, and Bireun. A linear discriminant analysis was then used to validate these results, and the linear discriminant analysis model derived from the cross-validation predicted the origin of Isotoma longiflora samples with 100 percent accuracy rate.CONCLUSION: The Isotoma longiflora leaf extracts were successfully classified using Fourier-transform infrared spectroscopy data processed through chemometric calculations (namely, principal component analysis). Based on the cross-validation using linear discriminant analysis showed that the prediction model had a 100 percent accuracy. The present study thus revealed the effect of geographical location on the composition of bioactive compounds in Isotoma longiflora, suggesting the potential of chemometric techniques for quality control and assurance in traditional medicine.
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spelling doaj-art-588cf3b01ed1454494ec407783ae99412025-02-03T05:44:18ZengGJESM PublisherGlobal Journal of Environmental Science and Management2383-35722383-38662024-01-0110110.22034/gjesm.2024.01.11705700Impact of environmental and geographical position on the chemometric classification of ethanol extracts from Isotoma longiflora leavesE. Imelda0K. Khairan1R.R. Lubis2T. Karma3R. Idroes4Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaDepartment of Ophthalmology, Faculty of Medicine, Universitas Sumatera Utara, Medan 20222, IndonesiaDepartment of Occupational Health and Safety, Faculty of Health Sciences, Abulyatama University, Banda Aceh 23372, IndonesiaDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaBACKGROUND AND OBJECTIVES: Isotoma longiflora L is commonly used as a medicinal plant by the local community in Indonesia, and its geographical position determines its bioactive compounds and hence its efficacy. Ethanol extracts of Isotoma longiflora leaves from various locations in Aceh Province were analyzed using a simple infrared spectroscopy technique combined with chemometrics to determine the effect of geographical location and conditions by classification and authentication.METHODS: Isotoma longiflora leaf samples were collected from Aceh Besar (a geothermal manifestation of Ie Suum), Banda Aceh, Aceh Jaya, Bireun, and Central Aceh. Principal component analysis was used to categorize the ethanol extract of Isotoma longiflora leaves, and a linear discriminant analysis was used for authentication.FINDINGS: The principal component analysis score plot indicated 89 percent of total data variance and that the samples formed three distinct groups: group I consisting of Aceh Tengah and Bener Meriah samples; group II of Aceh Besar and Banda Aceh samples; and group III of Aceh Selatan, Aceh Barat Daya, Aceh Jaya, and Bireun. A linear discriminant analysis was then used to validate these results, and the linear discriminant analysis model derived from the cross-validation predicted the origin of Isotoma longiflora samples with 100 percent accuracy rate.CONCLUSION: The Isotoma longiflora leaf extracts were successfully classified using Fourier-transform infrared spectroscopy data processed through chemometric calculations (namely, principal component analysis). Based on the cross-validation using linear discriminant analysis showed that the prediction model had a 100 percent accuracy. The present study thus revealed the effect of geographical location on the composition of bioactive compounds in Isotoma longiflora, suggesting the potential of chemometric techniques for quality control and assurance in traditional medicine.https://www.gjesm.net/article_705700_55a9d31fc3f2510cae6caf8643bd2e2b.pdfchemometricsisotoma longifloraspectroscopyprincipal component analysis
spellingShingle E. Imelda
K. Khairan
R.R. Lubis
T. Karma
R. Idroes
Impact of environmental and geographical position on the chemometric classification of ethanol extracts from Isotoma longiflora leaves
Global Journal of Environmental Science and Management
chemometrics
isotoma longiflora
spectroscopy
principal component analysis
title Impact of environmental and geographical position on the chemometric classification of ethanol extracts from Isotoma longiflora leaves
title_full Impact of environmental and geographical position on the chemometric classification of ethanol extracts from Isotoma longiflora leaves
title_fullStr Impact of environmental and geographical position on the chemometric classification of ethanol extracts from Isotoma longiflora leaves
title_full_unstemmed Impact of environmental and geographical position on the chemometric classification of ethanol extracts from Isotoma longiflora leaves
title_short Impact of environmental and geographical position on the chemometric classification of ethanol extracts from Isotoma longiflora leaves
title_sort impact of environmental and geographical position on the chemometric classification of ethanol extracts from isotoma longiflora leaves
topic chemometrics
isotoma longiflora
spectroscopy
principal component analysis
url https://www.gjesm.net/article_705700_55a9d31fc3f2510cae6caf8643bd2e2b.pdf
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