OsPRMT6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signaling
Abstract Plants rapidly induce strong abscisic acid (ABA) signaling in response to stress, but how they weaken ABA signaling to resume normal growth after stress is unclear. Here, we find that arginine methyltransferase 6b (OsPRMT6b) methylates three arginine residues (R48, R79, R113) in ABA recepto...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-06-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60350-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849469845409628160 |
|---|---|
| author | Xin Jin Qibing Lin Xinyue Zhang Shuang Zhang Weiwei Ma Peike Sheng Limin Zhang Minxi Wu Xudong Zhu Zhiwei Li Bojuan Liu Jiachang Wang Yupeng Wang Kun Dong Ming Yu Yulong Ren Zhijun Cheng Cailin Lei Xiuping Guo Xin Wang Haiyang Wang Fu-Qing Wu Shanshan Zhu Jianmin Wan |
| author_facet | Xin Jin Qibing Lin Xinyue Zhang Shuang Zhang Weiwei Ma Peike Sheng Limin Zhang Minxi Wu Xudong Zhu Zhiwei Li Bojuan Liu Jiachang Wang Yupeng Wang Kun Dong Ming Yu Yulong Ren Zhijun Cheng Cailin Lei Xiuping Guo Xin Wang Haiyang Wang Fu-Qing Wu Shanshan Zhu Jianmin Wan |
| author_sort | Xin Jin |
| collection | DOAJ |
| description | Abstract Plants rapidly induce strong abscisic acid (ABA) signaling in response to stress, but how they weaken ABA signaling to resume normal growth after stress is unclear. Here, we find that arginine methyltransferase 6b (OsPRMT6b) methylates three arginine residues (R48, R79, R113) in ABA receptor OsPYL/RCAR10 (OsPYR1-LIKE/REGULATORY COMPONENT OF ABA RECEPTOR, R10), thereby enhancing its interaction with Tiller Enhancer (TE) and promoting its ubiquitination and degradation through the 26S-proteasome pathway. OsPRMT6b is induced by ABA at both transcriptional and translational levels. Further, we find that R10 protein accumulates under high temperature stress but declines as temperature drops, whereas OsPRMT6b protein shows an opposite trend. And WT plants display a better growth recovery than osprmt6b mutants after high temperature. These findings suggest that OsPRMT6b acts as a switch to downregulate ABA signaling for growth recovery after high temperature stress. |
| format | Article |
| id | doaj-art-58839a0ee6b34a49b2f9b4f1d65c7d94 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-58839a0ee6b34a49b2f9b4f1d65c7d942025-08-20T03:25:19ZengNature PortfolioNature Communications2041-17232025-06-0116111610.1038/s41467-025-60350-yOsPRMT6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signalingXin Jin0Qibing Lin1Xinyue Zhang2Shuang Zhang3Weiwei Ma4Peike Sheng5Limin Zhang6Minxi Wu7Xudong Zhu8Zhiwei Li9Bojuan Liu10Jiachang Wang11Yupeng Wang12Kun Dong13Ming Yu14Yulong Ren15Zhijun Cheng16Cailin Lei17Xiuping Guo18Xin Wang19Haiyang Wang20Fu-Qing Wu21Shanshan Zhu22Jianmin Wan23State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural SciencesAbstract Plants rapidly induce strong abscisic acid (ABA) signaling in response to stress, but how they weaken ABA signaling to resume normal growth after stress is unclear. Here, we find that arginine methyltransferase 6b (OsPRMT6b) methylates three arginine residues (R48, R79, R113) in ABA receptor OsPYL/RCAR10 (OsPYR1-LIKE/REGULATORY COMPONENT OF ABA RECEPTOR, R10), thereby enhancing its interaction with Tiller Enhancer (TE) and promoting its ubiquitination and degradation through the 26S-proteasome pathway. OsPRMT6b is induced by ABA at both transcriptional and translational levels. Further, we find that R10 protein accumulates under high temperature stress but declines as temperature drops, whereas OsPRMT6b protein shows an opposite trend. And WT plants display a better growth recovery than osprmt6b mutants after high temperature. These findings suggest that OsPRMT6b acts as a switch to downregulate ABA signaling for growth recovery after high temperature stress.https://doi.org/10.1038/s41467-025-60350-y |
| spellingShingle | Xin Jin Qibing Lin Xinyue Zhang Shuang Zhang Weiwei Ma Peike Sheng Limin Zhang Minxi Wu Xudong Zhu Zhiwei Li Bojuan Liu Jiachang Wang Yupeng Wang Kun Dong Ming Yu Yulong Ren Zhijun Cheng Cailin Lei Xiuping Guo Xin Wang Haiyang Wang Fu-Qing Wu Shanshan Zhu Jianmin Wan OsPRMT6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signaling Nature Communications |
| title | OsPRMT6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signaling |
| title_full | OsPRMT6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signaling |
| title_fullStr | OsPRMT6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signaling |
| title_full_unstemmed | OsPRMT6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signaling |
| title_short | OsPRMT6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signaling |
| title_sort | osprmt6b balances plant growth and high temperature stress by feedback inhibition of abscisic acid signaling |
| url | https://doi.org/10.1038/s41467-025-60350-y |
| work_keys_str_mv | AT xinjin osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT qibinglin osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT xinyuezhang osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT shuangzhang osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT weiweima osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT peikesheng osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT liminzhang osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT minxiwu osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT xudongzhu osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT zhiweili osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT bojuanliu osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT jiachangwang osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT yupengwang osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT kundong osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT mingyu osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT yulongren osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT zhijuncheng osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT cailinlei osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT xiupingguo osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT xinwang osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT haiyangwang osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT fuqingwu osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT shanshanzhu osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling AT jianminwan osprmt6bbalancesplantgrowthandhightemperaturestressbyfeedbackinhibitionofabscisicacidsignaling |