Deletion polymorphism of glutathione S-transferases genes (GSTT1, GSTM1) in patients with breast cancer in Primorye region
Background. Breast cancer (BC) refers to multifactorial polygenic diseases that occur as a result of the combined interaction of genetic and environmental factors. Glutathione-mediated detoxification is of key importance in ensuring the resistance of body cells to the damaging effect of xenobiotics....
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| Series: | Опухоли женской репродуктивной системы |
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| Online Access: | https://ojrs.abvpress.ru/ojrs/article/view/763 |
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| author | I. S. Gulyan E. P. Bystritskaya N. Yu. Chernysheva E. V. Eliseeva V. I. Apanasevich M. P. Isaeva |
| author_facet | I. S. Gulyan E. P. Bystritskaya N. Yu. Chernysheva E. V. Eliseeva V. I. Apanasevich M. P. Isaeva |
| author_sort | I. S. Gulyan |
| collection | DOAJ |
| description | Background. Breast cancer (BC) refers to multifactorial polygenic diseases that occur as a result of the combined interaction of genetic and environmental factors. Glutathione-mediated detoxification is of key importance in ensuring the resistance of body cells to the damaging effect of xenobiotics.Objective: to study the prevalence of deletion polymorphisms of the GSTM1 and GSTT1 genes and to establish their influence on the formation of cancer risk in patients with BC in the Primorye region (Russia).Materials and methods. The study involved 176 women with BC, aged 23 to 79 years (mean age 48 ± 13 years) and 66 conditionally healthy individuals without cancer. The detection of deletion (null) genotypes of the GSTM1 and GSTT1 was carried out using multiplex PCR followed by analysis of the melting curves of the reaction products.Results. The frequency of GSTT1-0 genotype among BC patients was higher than in the control group (14.77 % versus 6.06 %), significantly exceeding the indicators in the control group by more than 2.5 times (p <0.1), indicating an association between the carriage of the GSTT1-0 genotype and the risk of developing BC. At the same time, the frequencies of the GSTM1-0 genotype in the study groups were comparable; no statistically significant association with the risk of developing BC was found.Conclusions. Homozygous deletion of GSTT1 (GSTT1-0) can potentially be considered as a low-penetrant risk factor for developing BC in the population of Primorye region. |
| format | Article |
| id | doaj-art-585c5ae2e4384239a1efd22938c217da |
| institution | Kabale University |
| issn | 1994-4098 1999-8627 |
| language | Russian |
| publishDate | 2021-01-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Опухоли женской репродуктивной системы |
| spelling | doaj-art-585c5ae2e4384239a1efd22938c217da2025-08-20T04:00:07ZrusABV-pressОпухоли женской репродуктивной системы1994-40981999-86272021-01-01163253110.17650/1994-4098-2020-16-3-25-31655Deletion polymorphism of glutathione S-transferases genes (GSTT1, GSTM1) in patients with breast cancer in Primorye regionI. S. Gulyan0E. P. Bystritskaya1N. Yu. Chernysheva2E. V. Eliseeva3V. I. Apanasevich4M. P. Isaeva5Pacific State Medical University; Far Eastern Federal UniversityG.B. Elyakov Pacific Institute of Bioorganic ChemistryG.B. Elyakov Pacific Institute of Bioorganic ChemistryPacific State Medical UniversityPacific State Medical UniversityG.B. Elyakov Pacific Institute of Bioorganic ChemistryBackground. Breast cancer (BC) refers to multifactorial polygenic diseases that occur as a result of the combined interaction of genetic and environmental factors. Glutathione-mediated detoxification is of key importance in ensuring the resistance of body cells to the damaging effect of xenobiotics.Objective: to study the prevalence of deletion polymorphisms of the GSTM1 and GSTT1 genes and to establish their influence on the formation of cancer risk in patients with BC in the Primorye region (Russia).Materials and methods. The study involved 176 women with BC, aged 23 to 79 years (mean age 48 ± 13 years) and 66 conditionally healthy individuals without cancer. The detection of deletion (null) genotypes of the GSTM1 and GSTT1 was carried out using multiplex PCR followed by analysis of the melting curves of the reaction products.Results. The frequency of GSTT1-0 genotype among BC patients was higher than in the control group (14.77 % versus 6.06 %), significantly exceeding the indicators in the control group by more than 2.5 times (p <0.1), indicating an association between the carriage of the GSTT1-0 genotype and the risk of developing BC. At the same time, the frequencies of the GSTM1-0 genotype in the study groups were comparable; no statistically significant association with the risk of developing BC was found.Conclusions. Homozygous deletion of GSTT1 (GSTT1-0) can potentially be considered as a low-penetrant risk factor for developing BC in the population of Primorye region.https://ojrs.abvpress.ru/ojrs/article/view/763breast cancergenetic polymorphismgstt1, gstm1 genes |
| spellingShingle | I. S. Gulyan E. P. Bystritskaya N. Yu. Chernysheva E. V. Eliseeva V. I. Apanasevich M. P. Isaeva Deletion polymorphism of glutathione S-transferases genes (GSTT1, GSTM1) in patients with breast cancer in Primorye region Опухоли женской репродуктивной системы breast cancer genetic polymorphism gstt1, gstm1 genes |
| title | Deletion polymorphism of glutathione S-transferases genes (GSTT1, GSTM1) in patients with breast cancer in Primorye region |
| title_full | Deletion polymorphism of glutathione S-transferases genes (GSTT1, GSTM1) in patients with breast cancer in Primorye region |
| title_fullStr | Deletion polymorphism of glutathione S-transferases genes (GSTT1, GSTM1) in patients with breast cancer in Primorye region |
| title_full_unstemmed | Deletion polymorphism of glutathione S-transferases genes (GSTT1, GSTM1) in patients with breast cancer in Primorye region |
| title_short | Deletion polymorphism of glutathione S-transferases genes (GSTT1, GSTM1) in patients with breast cancer in Primorye region |
| title_sort | deletion polymorphism of glutathione s transferases genes gstt1 gstm1 in patients with breast cancer in primorye region |
| topic | breast cancer genetic polymorphism gstt1, gstm1 genes |
| url | https://ojrs.abvpress.ru/ojrs/article/view/763 |
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