Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers
Abstract Diabetic foot ulcers (DFU) are a common and severe complication among diabetic patients, posing a significant burden on patients’ quality of life and healthcare systems due to their high incidence, amputation rates, and mortality. This study utilized single-cell RNA sequencing technology to...
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BMC
2025-03-01
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| Series: | Biology Direct |
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| Online Access: | https://doi.org/10.1186/s13062-025-00628-9 |
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| author | Songyun Zhao Hua Yu Zihao Li Wanying Chen Kaibo Liu Hao Dai Gaoyi Wang Zibing Zhang Jiaheng Xie Yucang He Liqun Li |
| author_facet | Songyun Zhao Hua Yu Zihao Li Wanying Chen Kaibo Liu Hao Dai Gaoyi Wang Zibing Zhang Jiaheng Xie Yucang He Liqun Li |
| author_sort | Songyun Zhao |
| collection | DOAJ |
| description | Abstract Diabetic foot ulcers (DFU) are a common and severe complication among diabetic patients, posing a significant burden on patients’ quality of life and healthcare systems due to their high incidence, amputation rates, and mortality. This study utilized single-cell RNA sequencing technology to deeply analyze the cellular heterogeneity of the skin on the feet ofDFU patients and the transcriptomic characteristics of endothelial cells, aiming to identify key cell populations and genes associated with the healing and progression of DFU. The study found that endothelial cells from DFU patients exhibited significant transcriptomic differences under various conditions, particularly in signaling pathways related to inflammatory responses and angiogenesis. Through trajectory analysis and cell communication research, we revealed the key role of endothelial cell subsets in the development of DFU and identified multiple important gene modules associated with the progression of DFU. Notably, the promoting effect of the SH3BGRL3 gene on endothelial cell proliferation, migration, and angiogenic capabilities under high glucose conditions was experimentally verified, providing a new potential target and theoretical basis for the treatment of DFU. This study not only enhances the understanding of the pathogenesis ofDFU but also provides a scientific basis for the development ofnew therapeutic strategies. |
| format | Article |
| id | doaj-art-5857e7cf3e254e949362e723f0a9ba45 |
| institution | Kabale University |
| issn | 1745-6150 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Biology Direct |
| spelling | doaj-art-5857e7cf3e254e949362e723f0a9ba452025-08-20T03:41:41ZengBMCBiology Direct1745-61502025-03-0120112110.1186/s13062-025-00628-9Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcersSongyun Zhao0Hua Yu1Zihao Li2Wanying Chen3Kaibo Liu4Hao Dai5Gaoyi Wang6Zibing Zhang7Jiaheng Xie8Yucang He9Liqun Li10Department of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityDepartment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityDepartment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityDepartment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityDepartment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityDepartment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityDepartment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical UniversityDepartment of Plastic Surgery, Xiangya Hospital, Central South UniversityDepartment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityDepartment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical UniversityAbstract Diabetic foot ulcers (DFU) are a common and severe complication among diabetic patients, posing a significant burden on patients’ quality of life and healthcare systems due to their high incidence, amputation rates, and mortality. This study utilized single-cell RNA sequencing technology to deeply analyze the cellular heterogeneity of the skin on the feet ofDFU patients and the transcriptomic characteristics of endothelial cells, aiming to identify key cell populations and genes associated with the healing and progression of DFU. The study found that endothelial cells from DFU patients exhibited significant transcriptomic differences under various conditions, particularly in signaling pathways related to inflammatory responses and angiogenesis. Through trajectory analysis and cell communication research, we revealed the key role of endothelial cell subsets in the development of DFU and identified multiple important gene modules associated with the progression of DFU. Notably, the promoting effect of the SH3BGRL3 gene on endothelial cell proliferation, migration, and angiogenic capabilities under high glucose conditions was experimentally verified, providing a new potential target and theoretical basis for the treatment of DFU. This study not only enhances the understanding of the pathogenesis ofDFU but also provides a scientific basis for the development ofnew therapeutic strategies.https://doi.org/10.1186/s13062-025-00628-9Diabetic foot ulcersSingle-cell RNA sequencingEndothelial cellsSH3BGRL3Wound healingAngiogenesis |
| spellingShingle | Songyun Zhao Hua Yu Zihao Li Wanying Chen Kaibo Liu Hao Dai Gaoyi Wang Zibing Zhang Jiaheng Xie Yucang He Liqun Li Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers Biology Direct Diabetic foot ulcers Single-cell RNA sequencing Endothelial cells SH3BGRL3 Wound healing Angiogenesis |
| title | Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers |
| title_full | Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers |
| title_fullStr | Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers |
| title_full_unstemmed | Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers |
| title_short | Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers |
| title_sort | single cell rna sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers |
| topic | Diabetic foot ulcers Single-cell RNA sequencing Endothelial cells SH3BGRL3 Wound healing Angiogenesis |
| url | https://doi.org/10.1186/s13062-025-00628-9 |
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