Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine.
Current influenza vaccines stimulate neutralising antibody to the haemagglutinin antigen but as there is antigenic drift in HA it is difficult to prepare a vaccine in advance against an emergent strain. A potential strategy is to induce CD8(+) and CD4(+) T cells that recognize epitopes within intern...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0062778&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850224781628014592 |
|---|---|
| author | Timothy J Powell Yanchun Peng Tamara K Berthoud Marie-Eve Blais Patrick J Lillie Adrian V S Hill Sarah L Rowland-Jones Andrew J McMichael Sarah C Gilbert Tao Dong |
| author_facet | Timothy J Powell Yanchun Peng Tamara K Berthoud Marie-Eve Blais Patrick J Lillie Adrian V S Hill Sarah L Rowland-Jones Andrew J McMichael Sarah C Gilbert Tao Dong |
| author_sort | Timothy J Powell |
| collection | DOAJ |
| description | Current influenza vaccines stimulate neutralising antibody to the haemagglutinin antigen but as there is antigenic drift in HA it is difficult to prepare a vaccine in advance against an emergent strain. A potential strategy is to induce CD8(+) and CD4(+) T cells that recognize epitopes within internal proteins that are less subject to antigenic drift. Augmenting humoral responses to HA with T cell responses to more conserved antigens may result in a more broadly protective vaccine. In this study, we evaluate the quality of influenza specific T cell responses in a clinical trial using MVA-NP+M1 vaccination followed by influenza virus challenge. In vaccinated volunteers, the expression of Granzyme A, Perforin and CD57 on influenza HLA A*02 M158-66 antigen specific cells was higher than non-vaccinated volunteers before and after challenge despite a similar frequency of antigen specific cells. BCL2 expression was lower in vaccinated volunteers. These data indicate that antigen specific T cells are a useful additional measure for use in human vaccination or immunization studies. |
| format | Article |
| id | doaj-art-58566ee2e0604f2381ddfca4bc296dde |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-58566ee2e0604f2381ddfca4bc296dde2025-08-20T02:05:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6277810.1371/journal.pone.0062778Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine.Timothy J PowellYanchun PengTamara K BerthoudMarie-Eve BlaisPatrick J LillieAdrian V S HillSarah L Rowland-JonesAndrew J McMichaelSarah C GilbertTao DongCurrent influenza vaccines stimulate neutralising antibody to the haemagglutinin antigen but as there is antigenic drift in HA it is difficult to prepare a vaccine in advance against an emergent strain. A potential strategy is to induce CD8(+) and CD4(+) T cells that recognize epitopes within internal proteins that are less subject to antigenic drift. Augmenting humoral responses to HA with T cell responses to more conserved antigens may result in a more broadly protective vaccine. In this study, we evaluate the quality of influenza specific T cell responses in a clinical trial using MVA-NP+M1 vaccination followed by influenza virus challenge. In vaccinated volunteers, the expression of Granzyme A, Perforin and CD57 on influenza HLA A*02 M158-66 antigen specific cells was higher than non-vaccinated volunteers before and after challenge despite a similar frequency of antigen specific cells. BCL2 expression was lower in vaccinated volunteers. These data indicate that antigen specific T cells are a useful additional measure for use in human vaccination or immunization studies.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0062778&type=printable |
| spellingShingle | Timothy J Powell Yanchun Peng Tamara K Berthoud Marie-Eve Blais Patrick J Lillie Adrian V S Hill Sarah L Rowland-Jones Andrew J McMichael Sarah C Gilbert Tao Dong Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine. PLoS ONE |
| title | Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine. |
| title_full | Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine. |
| title_fullStr | Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine. |
| title_full_unstemmed | Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine. |
| title_short | Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine. |
| title_sort | examination of influenza specific t cell responses after influenza virus challenge in individuals vaccinated with mva np m1 vaccine |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0062778&type=printable |
| work_keys_str_mv | AT timothyjpowell examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine AT yanchunpeng examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine AT tamarakberthoud examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine AT marieeveblais examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine AT patrickjlillie examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine AT adrianvshill examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine AT sarahlrowlandjones examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine AT andrewjmcmichael examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine AT sarahcgilbert examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine AT taodong examinationofinfluenzaspecifictcellresponsesafterinfluenzaviruschallengeinindividualsvaccinatedwithmvanpm1vaccine |