Regulation by anti-inflammatory cytokines (IL-4, IL-10, IL-13, TGFβ)of interleukin-8 production by LPS- and/ or TNFα-activated human polymorphonuclear cells
The capacity to down-regulate the production of IL-8 by LPS-activated human polymorphonuclear cells (PMN) has been demonstrated for IL-4, IL-10, and TGFβ. We compared their relative capacities and further extended this property to IL-13. We report a great heterogeneity among individuals related to t...
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| Format: | Article |
| Language: | English |
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Wiley
1996-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935196000488 |
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| author | C. Marie C. Pitton C. Fitting J-M. Cavaillon |
| author_facet | C. Marie C. Pitton C. Fitting J-M. Cavaillon |
| author_sort | C. Marie |
| collection | DOAJ |
| description | The capacity to down-regulate the production of IL-8 by LPS-activated human polymorphonuclear cells (PMN) has been demonstrated for IL-4, IL-10, and TGFβ. We compared their relative capacities and further extended this property to IL-13. We report a great heterogeneity among individuals related to the responsiveness of PMN to the IL-4 and IL-13 inhibitory effects while their response to the IL-10 effect was homogenous. The inhibitory activities were observed at the transcriptional level. IL-8 induction by TNFα was, unlike its induction by LPS, resistant to the inhibitory effects of IL-10, IL-4, IL-13 and TGFβ. Furthermore, IL-10 and IL-4 inhibitory activity were less effective when TNFα was acting synergistically with LPS to induce IL-8 production by PMN. LPS-induced cell-associated IL-8, detected in the PMN cultures, could be marginally inhibited by IL-4 and IL-10. Altogether, our data demonstrate that IL-13 is able to inhibit LPS-induced IL-8 production by human PMN, although IL-10 remains the most active anti-inflammatory cytokine. Despite the capacity of IL-4, IL-10, and IL-13 to limit the production of TNFα-induced IL-8 in a whole blood assay, none was able to inhibit this production when studying isolated human polymorphonuclear cells. |
| format | Article |
| id | doaj-art-58464d7dfd0f4c2d8eb74ef7a9225a3c |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-58464d7dfd0f4c2d8eb74ef7a9225a3c2025-08-20T02:05:48ZengWileyMediators of Inflammation0962-93511466-18611996-01-015533434010.1155/S0962935196000488Regulation by anti-inflammatory cytokines (IL-4, IL-10, IL-13, TGFβ)of interleukin-8 production by LPS- and/ or TNFα-activated human polymorphonuclear cellsC. Marie0C. Pitton1C. Fitting2J-M. Cavaillon3Unité d'lmmuno-Allergie, Institut Pasteur, 28 rue Dr Roux, Paris cedex 15 75724, FranceUnité d'lmmuno-Allergie, Institut Pasteur, 28 rue Dr Roux, Paris cedex 15 75724, FranceUnité d'lmmuno-Allergie, Institut Pasteur, 28 rue Dr Roux, Paris cedex 15 75724, FranceUnité d'lmmuno-Allergie, Institut Pasteur, 28 rue Dr Roux, Paris cedex 15 75724, FranceThe capacity to down-regulate the production of IL-8 by LPS-activated human polymorphonuclear cells (PMN) has been demonstrated for IL-4, IL-10, and TGFβ. We compared their relative capacities and further extended this property to IL-13. We report a great heterogeneity among individuals related to the responsiveness of PMN to the IL-4 and IL-13 inhibitory effects while their response to the IL-10 effect was homogenous. The inhibitory activities were observed at the transcriptional level. IL-8 induction by TNFα was, unlike its induction by LPS, resistant to the inhibitory effects of IL-10, IL-4, IL-13 and TGFβ. Furthermore, IL-10 and IL-4 inhibitory activity were less effective when TNFα was acting synergistically with LPS to induce IL-8 production by PMN. LPS-induced cell-associated IL-8, detected in the PMN cultures, could be marginally inhibited by IL-4 and IL-10. Altogether, our data demonstrate that IL-13 is able to inhibit LPS-induced IL-8 production by human PMN, although IL-10 remains the most active anti-inflammatory cytokine. Despite the capacity of IL-4, IL-10, and IL-13 to limit the production of TNFα-induced IL-8 in a whole blood assay, none was able to inhibit this production when studying isolated human polymorphonuclear cells.http://dx.doi.org/10.1155/S0962935196000488 |
| spellingShingle | C. Marie C. Pitton C. Fitting J-M. Cavaillon Regulation by anti-inflammatory cytokines (IL-4, IL-10, IL-13, TGFβ)of interleukin-8 production by LPS- and/ or TNFα-activated human polymorphonuclear cells Mediators of Inflammation |
| title | Regulation by anti-inflammatory cytokines (IL-4, IL-10, IL-13, TGFβ)of interleukin-8 production by LPS- and/ or TNFα-activated human polymorphonuclear cells |
| title_full | Regulation by anti-inflammatory cytokines (IL-4, IL-10, IL-13, TGFβ)of interleukin-8 production by LPS- and/ or TNFα-activated human polymorphonuclear cells |
| title_fullStr | Regulation by anti-inflammatory cytokines (IL-4, IL-10, IL-13, TGFβ)of interleukin-8 production by LPS- and/ or TNFα-activated human polymorphonuclear cells |
| title_full_unstemmed | Regulation by anti-inflammatory cytokines (IL-4, IL-10, IL-13, TGFβ)of interleukin-8 production by LPS- and/ or TNFα-activated human polymorphonuclear cells |
| title_short | Regulation by anti-inflammatory cytokines (IL-4, IL-10, IL-13, TGFβ)of interleukin-8 production by LPS- and/ or TNFα-activated human polymorphonuclear cells |
| title_sort | regulation by anti inflammatory cytokines il 4 il 10 il 13 tgfβ of interleukin 8 production by lps and or tnfα activated human polymorphonuclear cells |
| url | http://dx.doi.org/10.1155/S0962935196000488 |
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