Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system

Summary: A non-invasive system can measure nutrient uptake and photosynthate partitioning simultaneously while allowing organisms to metabolize without interference in the process of measurement. Here, we present a protocol for detecting nutrient uptake and photosynthate partitioning in rice seedlin...

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Main Authors: Jinzhi Li, He Zhang, Jianming Gao
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001133
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author Jinzhi Li
He Zhang
Jianming Gao
author_facet Jinzhi Li
He Zhang
Jianming Gao
author_sort Jinzhi Li
collection DOAJ
description Summary: A non-invasive system can measure nutrient uptake and photosynthate partitioning simultaneously while allowing organisms to metabolize without interference in the process of measurement. Here, we present a protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system. We describe steps for preparing plant material and material for the non-invasive system. We then detail procedures for setting up and applying the non-invasive measurement system.For complete details on the use and execution of this protocol, please refer to Li et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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spelling doaj-art-583c7e0befa14dee93c65613f06b933b2025-08-20T01:55:11ZengElsevierSTAR Protocols2666-16672025-06-016210370710.1016/j.xpro.2025.103707Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive systemJinzhi Li0He Zhang1Jianming Gao2Jiangxi Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, China; Corresponding authorJiangxi Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, ChinaInstitute of Tropical Bioscience and Biotechnology/Hainan Institute of Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; Corresponding authorSummary: A non-invasive system can measure nutrient uptake and photosynthate partitioning simultaneously while allowing organisms to metabolize without interference in the process of measurement. Here, we present a protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system. We describe steps for preparing plant material and material for the non-invasive system. We then detail procedures for setting up and applying the non-invasive measurement system.For complete details on the use and execution of this protocol, please refer to Li et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725001133MetabolismPlant sciences
spellingShingle Jinzhi Li
He Zhang
Jianming Gao
Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system
STAR Protocols
Metabolism
Plant sciences
title Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system
title_full Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system
title_fullStr Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system
title_full_unstemmed Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system
title_short Protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non-invasive system
title_sort protocol for detecting nutrient uptake and photosynthate partitioning in rice seedling using a non invasive system
topic Metabolism
Plant sciences
url http://www.sciencedirect.com/science/article/pii/S2666166725001133
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