miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro
(1) Background: Endometriosis is a highly prevalent gynecological disease affecting 10% of women of reproductive age worldwide. miRNAs may play a role in endometriosis, though their exact function remains unclear. This study aimed to identify differentially expressed miRNAs in endometriosis and stud...
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2024-11-01
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| author | Caroline Frisendahl Yiqun Tang Nageswara Rao Boggavarapu Maire Peters Parameswaran Grace Lalitkumar Terhi T. Piltonen Riikka K. Arffman Andres Salumets Martin Götte Eberhard Korsching Kristina Gemzell-Danielsson |
| author_facet | Caroline Frisendahl Yiqun Tang Nageswara Rao Boggavarapu Maire Peters Parameswaran Grace Lalitkumar Terhi T. Piltonen Riikka K. Arffman Andres Salumets Martin Götte Eberhard Korsching Kristina Gemzell-Danielsson |
| author_sort | Caroline Frisendahl |
| collection | DOAJ |
| description | (1) Background: Endometriosis is a highly prevalent gynecological disease affecting 10% of women of reproductive age worldwide. miRNAs may play a role in endometriosis, though their exact function remains unclear. This study aimed to identify differentially expressed miRNAs in endometriosis and study their functions in the disease. (2) Methods: Endometrial tissue was collected from women with endometriosis (n = 15) and non-endometriosis controls (n = 17). Dysregulated miRNAs were identified through small RNA-sequencing, and their biological significance was explored by target gene prediction and pathway analysis. Selected miRNAs were examined in paired ectopic endometriomas and eutopic endometrium (n = 10) using qRT-PCR. Their roles in cell migration and proliferation were further examined in vitro using functional assays. To identify potential target genes, we performed mRNA sequencing on transfected cells and the endometrioma cohort. (3) Results: We identified 14 dysregulated miRNAs in the eutopic endometrium of women with endometriosis compared to endometrial tissue from women without endometriosis. Pathway analysis indicated enrichment in cell migration and proliferation-associated pathways. Further ex vivo studies of miR-193b-5p and miR-374b-5p showed that both miRNAs were upregulated in endometrioma. Overexpression of these two miRNAs in vitro inhibited cell migration, and mRNA sequencing revealed several migration-related genes that are targeted by these miRNAs. (4) Conclusions: Our study identified two key endometrial miRNAs that may be involved in the pathogenesis of endometriosis by regulating cell migration. |
| format | Article |
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| institution | OA Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-583a70fc8cff4bddb84bfc1150e525532025-08-20T02:28:02ZengMDPI AGBiomolecules2218-273X2024-11-011411140010.3390/biom14111400miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In VitroCaroline Frisendahl0Yiqun Tang1Nageswara Rao Boggavarapu2Maire Peters3Parameswaran Grace Lalitkumar4Terhi T. Piltonen5Riikka K. Arffman6Andres Salumets7Martin Götte8Eberhard Korsching9Kristina Gemzell-Danielsson10WHO Collaborating Centre, Division of Neonatology, Obstetrics, Gynecology, and Reproductive Health, Department of Women’s and Children’s Health, Karolinska University Hospital, Karolinska Institutet, SE 17176 Stockholm, SwedenWHO Collaborating Centre, Division of Neonatology, Obstetrics, Gynecology, and Reproductive Health, Department of Women’s and Children’s Health, Karolinska University Hospital, Karolinska Institutet, SE 17176 Stockholm, SwedenWHO Collaborating Centre, Division of Neonatology, Obstetrics, Gynecology, and Reproductive Health, Department of Women’s and Children’s Health, Karolinska University Hospital, Karolinska Institutet, SE 17176 Stockholm, SwedenDepartment of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, 50406 Tartu, EstoniaWHO Collaborating Centre, Division of Neonatology, Obstetrics, Gynecology, and Reproductive Health, Department of Women’s and Children’s Health, Karolinska University Hospital, Karolinska Institutet, SE 17176 Stockholm, SwedenDepartment of Obstetrics and Gynecology, Research Unit of Clinical Medicine, Medical Research Centre, Oulu University Hospital, University of Oulu, 90220 Oulu, FinlandDepartment of Obstetrics and Gynecology, Research Unit of Clinical Medicine, Medical Research Centre, Oulu University Hospital, University of Oulu, 90220 Oulu, FinlandDepartment of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, 50406 Tartu, EstoniaDepartment of Gynecology and Obstetrics, University Hospital of Münster, University of Münster, 48149 Münster, GermanyInstitute of Bioinformatics, University Hospital of Münster, University of Münster, 48149 Münster, GermanyWHO Collaborating Centre, Division of Neonatology, Obstetrics, Gynecology, and Reproductive Health, Department of Women’s and Children’s Health, Karolinska University Hospital, Karolinska Institutet, SE 17176 Stockholm, Sweden(1) Background: Endometriosis is a highly prevalent gynecological disease affecting 10% of women of reproductive age worldwide. miRNAs may play a role in endometriosis, though their exact function remains unclear. This study aimed to identify differentially expressed miRNAs in endometriosis and study their functions in the disease. (2) Methods: Endometrial tissue was collected from women with endometriosis (n = 15) and non-endometriosis controls (n = 17). Dysregulated miRNAs were identified through small RNA-sequencing, and their biological significance was explored by target gene prediction and pathway analysis. Selected miRNAs were examined in paired ectopic endometriomas and eutopic endometrium (n = 10) using qRT-PCR. Their roles in cell migration and proliferation were further examined in vitro using functional assays. To identify potential target genes, we performed mRNA sequencing on transfected cells and the endometrioma cohort. (3) Results: We identified 14 dysregulated miRNAs in the eutopic endometrium of women with endometriosis compared to endometrial tissue from women without endometriosis. Pathway analysis indicated enrichment in cell migration and proliferation-associated pathways. Further ex vivo studies of miR-193b-5p and miR-374b-5p showed that both miRNAs were upregulated in endometrioma. Overexpression of these two miRNAs in vitro inhibited cell migration, and mRNA sequencing revealed several migration-related genes that are targeted by these miRNAs. (4) Conclusions: Our study identified two key endometrial miRNAs that may be involved in the pathogenesis of endometriosis by regulating cell migration.https://www.mdpi.com/2218-273X/14/11/1400endometriosisendometriumendometriomasmall RNA sequencingmRNA sequencingmicroRNAs |
| spellingShingle | Caroline Frisendahl Yiqun Tang Nageswara Rao Boggavarapu Maire Peters Parameswaran Grace Lalitkumar Terhi T. Piltonen Riikka K. Arffman Andres Salumets Martin Götte Eberhard Korsching Kristina Gemzell-Danielsson miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro Biomolecules endometriosis endometrium endometrioma small RNA sequencing mRNA sequencing microRNAs |
| title | miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro |
| title_full | miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro |
| title_fullStr | miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro |
| title_full_unstemmed | miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro |
| title_short | miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro |
| title_sort | mir 193b 5p and mir 374b 5p are aberrantly expressed in endometriosis and suppress endometrial cell migration in vitro |
| topic | endometriosis endometrium endometrioma small RNA sequencing mRNA sequencing microRNAs |
| url | https://www.mdpi.com/2218-273X/14/11/1400 |
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