EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS
Abstract. The study deals with evaluation of dehydroepiandrosterone sulfate (DHEAS) effects upon phenotype and functions of dendritic cells (DCs), generated from blood monocytes of pregnant women by means of GM-CSF and IFN-alpha. It was shown, that initial supplementation with DHEAS (10-6 M, day 1 o...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/245 |
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| author | N. V. Seledzova N. A. Khonina M. A. Tikhonova A. A. Ostanin N. M. Passman E. R. Chernykh |
| author_facet | N. V. Seledzova N. A. Khonina M. A. Tikhonova A. A. Ostanin N. M. Passman E. R. Chernykh |
| author_sort | N. V. Seledzova |
| collection | DOAJ |
| description | Abstract. The study deals with evaluation of dehydroepiandrosterone sulfate (DHEAS) effects upon phenotype and functions of dendritic cells (DCs), generated from blood monocytes of pregnant women by means of GM-CSF and IFN-alpha. It was shown, that initial supplementation with DHEAS (10-6 M, day 1 of culture) did not influence the pattern of DC subsets. Meanwhile, addition of DHEAS at the stage of DC maturation (last day of culture) is accompanied by a significant increase in mature CD83+ cells and activated DCs (CD25+), like as their precursors (CD1a+). Furthermore, the DCs generated in presence of DHEAS were characterized by marked allostimulating activity and decreased ability to downregulate the numbers of CD56+CD16+ NK cells. Hence, DHEAS promotes generation, maturation, and allostimulating activity of DCs, along with decreased negative regulation towards CD56+CD16+NK cell amounts. DHEAS-mediated changes in DCs’ phenotype and functioning are discussed as a possible mechanism of disturbed immunological tolerance to fetal antigens in pregnant women with suprarenal hyperandrogenia. |
| format | Article |
| id | doaj-art-58355f1ce1d446f0b07adaeae0b2efb3 |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-58355f1ce1d446f0b07adaeae0b2efb32025-08-20T04:00:09ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-019658959610.15789/1563-0625-2007-6-589-596242EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLSN. V. Seledzova0N. A. Khonina1M. A. Tikhonova2A. A. Ostanin3N. M. Passman4E. R. Chernykh5ГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскAbstract. The study deals with evaluation of dehydroepiandrosterone sulfate (DHEAS) effects upon phenotype and functions of dendritic cells (DCs), generated from blood monocytes of pregnant women by means of GM-CSF and IFN-alpha. It was shown, that initial supplementation with DHEAS (10-6 M, day 1 of culture) did not influence the pattern of DC subsets. Meanwhile, addition of DHEAS at the stage of DC maturation (last day of culture) is accompanied by a significant increase in mature CD83+ cells and activated DCs (CD25+), like as their precursors (CD1a+). Furthermore, the DCs generated in presence of DHEAS were characterized by marked allostimulating activity and decreased ability to downregulate the numbers of CD56+CD16+ NK cells. Hence, DHEAS promotes generation, maturation, and allostimulating activity of DCs, along with decreased negative regulation towards CD56+CD16+NK cell amounts. DHEAS-mediated changes in DCs’ phenotype and functioning are discussed as a possible mechanism of disturbed immunological tolerance to fetal antigens in pregnant women with suprarenal hyperandrogenia.https://www.mimmun.ru/mimmun/article/view/245dehydroepiandrosterone sulfatedendritic cellsnk-cellshyperandrogenia |
| spellingShingle | N. V. Seledzova N. A. Khonina M. A. Tikhonova A. A. Ostanin N. M. Passman E. R. Chernykh EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS Медицинская иммунология dehydroepiandrosterone sulfate dendritic cells nk-cells hyperandrogenia |
| title | EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS |
| title_full | EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS |
| title_fullStr | EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS |
| title_full_unstemmed | EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS |
| title_short | EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS |
| title_sort | effects of dehydroepiandrosterone sulfate upon phenotype and in vitro functions of dendritic cells |
| topic | dehydroepiandrosterone sulfate dendritic cells nk-cells hyperandrogenia |
| url | https://www.mimmun.ru/mimmun/article/view/245 |
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