Neural G0: a quiescent‐like state found in neuroepithelial‐derived cells and glioma
Abstract Single‐cell RNA sequencing has emerged as a powerful tool for resolving cellular states associated with normal and maligned developmental processes. Here, we used scRNA‐seq to examine the cell cycle states of expanding human neural stem cells (hNSCs). From these data, we constructed a cell...
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| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2021-06-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.15252/msb.20209522 |
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| author | Samantha A O’Connor Heather M Feldman Sonali Arora Pia Hoellerbauer Chad M Toledo Philip Corrin Lucas Carter Megan Kufeld Hamid Bolouri Ryan Basom Jeffrey Delrow José L McFaline‐Figueroa Cole Trapnell Steven M Pollard Anoop Patel Patrick J Paddison Christopher L Plaisier |
| author_facet | Samantha A O’Connor Heather M Feldman Sonali Arora Pia Hoellerbauer Chad M Toledo Philip Corrin Lucas Carter Megan Kufeld Hamid Bolouri Ryan Basom Jeffrey Delrow José L McFaline‐Figueroa Cole Trapnell Steven M Pollard Anoop Patel Patrick J Paddison Christopher L Plaisier |
| author_sort | Samantha A O’Connor |
| collection | DOAJ |
| description | Abstract Single‐cell RNA sequencing has emerged as a powerful tool for resolving cellular states associated with normal and maligned developmental processes. Here, we used scRNA‐seq to examine the cell cycle states of expanding human neural stem cells (hNSCs). From these data, we constructed a cell cycle classifier that identifies traditional cell cycle phases and a putative quiescent‐like state in neuroepithelial‐derived cell types during mammalian neurogenesis and in gliomas. The Neural G0 markers are enriched with quiescent NSC genes and other neurodevelopmental markers found in non‐dividing neural progenitors. Putative glioblastoma stem‐like cells were significantly enriched in the Neural G0 cell population. Neural G0 cell populations and gene expression are significantly associated with less aggressive tumors and extended patient survival for gliomas. Genetic screens to identify modulators of Neural G0 revealed that knockout of genes associated with the Hippo/Yap and p53 pathways diminished Neural G0 in vitro, resulting in faster G1 transit, down‐regulation of quiescence‐associated markers, and loss of Neural G0 gene expression. Thus, Neural G0 represents a dynamic quiescent‐like state found in neuroepithelial‐derived cells and gliomas. |
| format | Article |
| id | doaj-art-58249d732dc642c29b1b9c5be97d78eb |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2021-06-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-58249d732dc642c29b1b9c5be97d78eb2025-08-20T03:42:04ZengSpringer NatureMolecular Systems Biology1744-42922021-06-0117612910.15252/msb.20209522Neural G0: a quiescent‐like state found in neuroepithelial‐derived cells and gliomaSamantha A O’Connor0Heather M Feldman1Sonali Arora2Pia Hoellerbauer3Chad M Toledo4Philip Corrin5Lucas Carter6Megan Kufeld7Hamid Bolouri8Ryan Basom9Jeffrey Delrow10José L McFaline‐Figueroa11Cole Trapnell12Steven M Pollard13Anoop Patel14Patrick J Paddison15Christopher L Plaisier16School of Biological and Health Systems Engineering, Arizona State UniversityHuman Biology Division, Fred Hutchinson Cancer Research CenterHuman Biology Division, Fred Hutchinson Cancer Research CenterHuman Biology Division, Fred Hutchinson Cancer Research CenterHuman Biology Division, Fred Hutchinson Cancer Research CenterHuman Biology Division, Fred Hutchinson Cancer Research CenterHuman Biology Division, Fred Hutchinson Cancer Research CenterHuman Biology Division, Fred Hutchinson Cancer Research CenterHuman Biology Division, Fred Hutchinson Cancer Research CenterGenomics and Bioinformatics Shared Resources, Fred Hutchinson Cancer Research CenterGenomics and Bioinformatics Shared Resources, Fred Hutchinson Cancer Research CenterDepartment of Genome Sciences, University of WashingtonDepartment of Genome Sciences, University of WashingtonEdinburgh CRUK Cancer Research Centre, MRC Centre for Regenerative Medicine, The University of EdinburghHuman Biology Division, Fred Hutchinson Cancer Research CenterHuman Biology Division, Fred Hutchinson Cancer Research CenterSchool of Biological and Health Systems Engineering, Arizona State UniversityAbstract Single‐cell RNA sequencing has emerged as a powerful tool for resolving cellular states associated with normal and maligned developmental processes. Here, we used scRNA‐seq to examine the cell cycle states of expanding human neural stem cells (hNSCs). From these data, we constructed a cell cycle classifier that identifies traditional cell cycle phases and a putative quiescent‐like state in neuroepithelial‐derived cell types during mammalian neurogenesis and in gliomas. The Neural G0 markers are enriched with quiescent NSC genes and other neurodevelopmental markers found in non‐dividing neural progenitors. Putative glioblastoma stem‐like cells were significantly enriched in the Neural G0 cell population. Neural G0 cell populations and gene expression are significantly associated with less aggressive tumors and extended patient survival for gliomas. Genetic screens to identify modulators of Neural G0 revealed that knockout of genes associated with the Hippo/Yap and p53 pathways diminished Neural G0 in vitro, resulting in faster G1 transit, down‐regulation of quiescence‐associated markers, and loss of Neural G0 gene expression. Thus, Neural G0 represents a dynamic quiescent‐like state found in neuroepithelial‐derived cells and gliomas.https://doi.org/10.15252/msb.20209522G0gliomaneural stem cellsquiescencescRNA‐seq |
| spellingShingle | Samantha A O’Connor Heather M Feldman Sonali Arora Pia Hoellerbauer Chad M Toledo Philip Corrin Lucas Carter Megan Kufeld Hamid Bolouri Ryan Basom Jeffrey Delrow José L McFaline‐Figueroa Cole Trapnell Steven M Pollard Anoop Patel Patrick J Paddison Christopher L Plaisier Neural G0: a quiescent‐like state found in neuroepithelial‐derived cells and glioma Molecular Systems Biology G0 glioma neural stem cells quiescence scRNA‐seq |
| title | Neural G0: a quiescent‐like state found in neuroepithelial‐derived cells and glioma |
| title_full | Neural G0: a quiescent‐like state found in neuroepithelial‐derived cells and glioma |
| title_fullStr | Neural G0: a quiescent‐like state found in neuroepithelial‐derived cells and glioma |
| title_full_unstemmed | Neural G0: a quiescent‐like state found in neuroepithelial‐derived cells and glioma |
| title_short | Neural G0: a quiescent‐like state found in neuroepithelial‐derived cells and glioma |
| title_sort | neural g0 a quiescent like state found in neuroepithelial derived cells and glioma |
| topic | G0 glioma neural stem cells quiescence scRNA‐seq |
| url | https://doi.org/10.15252/msb.20209522 |
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