A perfusion decellularization heart model - an interesting tool for cell-matrix interaction studies
Objective. In this paper, we present a technique for whole human, pig, or rat heart decellularization in order to obtain a tool for cell-matrix interaction studies and to obtain the extracellular matrix scaffold with applications to the study of cardiac connective tissue architecture for tissue bioe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Journal of Mind and Medical Sciences |
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| Online Access: | https://scholar.valpo.edu/cgi/viewcontent.cgi?article=1144&context=jmms |
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| author | Mihai Meșină Ion Mîndrilă Cristian Meșină Cosmin Vasile Obleagă Octavian Istrătoaie |
| author_facet | Mihai Meșină Ion Mîndrilă Cristian Meșină Cosmin Vasile Obleagă Octavian Istrătoaie |
| author_sort | Mihai Meșină |
| collection | DOAJ |
| description | Objective. In this paper, we present a technique for whole human, pig, or rat heart decellularization in order to obtain a tool for cell-matrix interaction studies and to obtain the extracellular matrix scaffold with applications to the study of cardiac connective tissue architecture for tissue bioengineering. Material and methods. In order to achieve tissue decellularization we present two separate protocols, the first for large animal hearts (e.g., pig hearts) and human hearts, the second for smaller hearts (e.g., rat hearts). We have performed the cardiac decellularization technique on 20 pig hearts, 5 human hearts, and 20 rat hearts. Results. The decellularization technique on the heart was assessed through histological examination. Conclusion. Although the decellularization technique on the heart is currently under development, this process affords the possibility of developing research in the fields of biomaterials, tissue engineering, and cardiac cell cultures. |
| format | Article |
| id | doaj-art-581de5e461e44e89a127158f4266c024 |
| institution | DOAJ |
| issn | 2392-7674 |
| language | English |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Mind and Medical Sciences |
| spelling | doaj-art-581de5e461e44e89a127158f4266c0242025-08-20T03:19:34ZengMDPI AGJournal of Mind and Medical Sciences2392-76742019-05-016113714210.22543/7674.61.P137142A perfusion decellularization heart model - an interesting tool for cell-matrix interaction studiesMihai MeșinăIon MîndrilăCristian MeșinăCosmin Vasile ObleagăOctavian IstrătoaieObjective. In this paper, we present a technique for whole human, pig, or rat heart decellularization in order to obtain a tool for cell-matrix interaction studies and to obtain the extracellular matrix scaffold with applications to the study of cardiac connective tissue architecture for tissue bioengineering. Material and methods. In order to achieve tissue decellularization we present two separate protocols, the first for large animal hearts (e.g., pig hearts) and human hearts, the second for smaller hearts (e.g., rat hearts). We have performed the cardiac decellularization technique on 20 pig hearts, 5 human hearts, and 20 rat hearts. Results. The decellularization technique on the heart was assessed through histological examination. Conclusion. Although the decellularization technique on the heart is currently under development, this process affords the possibility of developing research in the fields of biomaterials, tissue engineering, and cardiac cell cultures.https://scholar.valpo.edu/cgi/viewcontent.cgi?article=1144&context=jmmsdecellularization techniquetissue engineeringcardiac cell cultures. |
| spellingShingle | Mihai Meșină Ion Mîndrilă Cristian Meșină Cosmin Vasile Obleagă Octavian Istrătoaie A perfusion decellularization heart model - an interesting tool for cell-matrix interaction studies Journal of Mind and Medical Sciences decellularization technique tissue engineering cardiac cell cultures. |
| title | A perfusion decellularization heart model - an interesting tool for cell-matrix interaction studies |
| title_full | A perfusion decellularization heart model - an interesting tool for cell-matrix interaction studies |
| title_fullStr | A perfusion decellularization heart model - an interesting tool for cell-matrix interaction studies |
| title_full_unstemmed | A perfusion decellularization heart model - an interesting tool for cell-matrix interaction studies |
| title_short | A perfusion decellularization heart model - an interesting tool for cell-matrix interaction studies |
| title_sort | perfusion decellularization heart model an interesting tool for cell matrix interaction studies |
| topic | decellularization technique tissue engineering cardiac cell cultures. |
| url | https://scholar.valpo.edu/cgi/viewcontent.cgi?article=1144&context=jmms |
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